Tags

Type your tag names separated by a space and hit enter

Dangguijakyak-san protects dopamine neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity under postmenopausal conditions.
J Ethnopharmacol. 2012 Feb 15; 139(3):883-8.JE

Abstract

Dangguijakyak-san protects dopamine neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity under postmenopausal conditions.

ETHNOPHARMACOLOGICAL RELEVANCE

Dangguijakyak-san (DJS), a famous traditional herbal formula, has long been used to treat gynecological disorders, including postmenopausal symptoms. This study evaluated the effects and mechanism of DJS on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity in a postmenopausal mouse model induced by ovariectomy.

MATERIALS AND METHODS

Three weeks after ovariectomy, C57bl/6 female mice were divided randomly into (1) control, (2) MPTP (30 mg/kg/day, i.p., 5 days), (3) MPTP+estrogen (50 μg/kg/day, i.p., 5 days), and (4) MPTP+DJS (50 mg/kg/day, p.o., 5 days) groups. We investigated the behavioral recovery and dopamine neuron protection of DJS using the pole test and tyrosine hydroxylase (TH) immunohistochemistry. We also explored the mechanism by assessing the protein expression of Bax, Bcl-2, cytochrome c, and cleaved caspase-3.

RESULTS

DJS treatment restored the movement behavior impaired by MPTP, showing a similar or better effect than estrogen. DJS protected TH-immunoreactive cells and fibers in the nigrostriatal region from MPTP toxicity. In addition, DJS inhibited the Bcl-2 decrease and Bax increase in mitochondria, cytochrome c release to the cytosol, and caspase-3 activation induced by MPTP.

CONCLUSIONS

DJS showed behavior recovery and dopamine neuron protection against MPTP-induced toxicity via anti-apoptotic activities in ovariectomized female mice. These results suggest that DJS treatment is effective for postmenopausal neurodegenerative diseases.

Authors+Show Affiliations

Department of Oriental Gynecology, College of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22178176

Citation

Lee, Jin-Moo, et al. "Dangguijakyak-san Protects Dopamine Neurons Against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Neurotoxicity Under Postmenopausal Conditions." Journal of Ethnopharmacology, vol. 139, no. 3, 2012, pp. 883-8.
Lee JM, Hwang DS, Kim HG, et al. Dangguijakyak-san protects dopamine neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity under postmenopausal conditions. J Ethnopharmacol. 2012;139(3):883-8.
Lee, J. M., Hwang, D. S., Kim, H. G., Lee, C. H., & Oh, M. S. (2012). Dangguijakyak-san protects dopamine neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity under postmenopausal conditions. Journal of Ethnopharmacology, 139(3), 883-8. https://doi.org/10.1016/j.jep.2011.12.015
Lee JM, et al. Dangguijakyak-san Protects Dopamine Neurons Against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Neurotoxicity Under Postmenopausal Conditions. J Ethnopharmacol. 2012 Feb 15;139(3):883-8. PubMed PMID: 22178176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dangguijakyak-san protects dopamine neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity under postmenopausal conditions. AU - Lee,Jin-Moo, AU - Hwang,Deok-Sang, AU - Kim,Hyo Geun, AU - Lee,Chang-Hoon, AU - Oh,Myung Sook, Y1 - 2011/12/13/ PY - 2011/09/01/received PY - 2011/11/03/revised PY - 2011/12/03/accepted PY - 2011/12/20/entrez PY - 2011/12/20/pubmed PY - 2012/6/14/medline SP - 883 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 139 IS - 3 N2 - UNLABELLED: Dangguijakyak-san protects dopamine neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity under postmenopausal conditions. ETHNOPHARMACOLOGICAL RELEVANCE: Dangguijakyak-san (DJS), a famous traditional herbal formula, has long been used to treat gynecological disorders, including postmenopausal symptoms. This study evaluated the effects and mechanism of DJS on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity in a postmenopausal mouse model induced by ovariectomy. MATERIALS AND METHODS: Three weeks after ovariectomy, C57bl/6 female mice were divided randomly into (1) control, (2) MPTP (30 mg/kg/day, i.p., 5 days), (3) MPTP+estrogen (50 μg/kg/day, i.p., 5 days), and (4) MPTP+DJS (50 mg/kg/day, p.o., 5 days) groups. We investigated the behavioral recovery and dopamine neuron protection of DJS using the pole test and tyrosine hydroxylase (TH) immunohistochemistry. We also explored the mechanism by assessing the protein expression of Bax, Bcl-2, cytochrome c, and cleaved caspase-3. RESULTS: DJS treatment restored the movement behavior impaired by MPTP, showing a similar or better effect than estrogen. DJS protected TH-immunoreactive cells and fibers in the nigrostriatal region from MPTP toxicity. In addition, DJS inhibited the Bcl-2 decrease and Bax increase in mitochondria, cytochrome c release to the cytosol, and caspase-3 activation induced by MPTP. CONCLUSIONS: DJS showed behavior recovery and dopamine neuron protection against MPTP-induced toxicity via anti-apoptotic activities in ovariectomized female mice. These results suggest that DJS treatment is effective for postmenopausal neurodegenerative diseases. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22178176/Dangguijakyak_san_protects_dopamine_neurons_against_1_methyl_4_phenyl_1236_tetrahydropyridine_induced_neurotoxicity_under_postmenopausal_conditions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00882-8 DB - PRIME DP - Unbound Medicine ER -