Tags

Type your tag names separated by a space and hit enter

Campomanesia lineatifolia Ruiz & Pav. as a gastroprotective agent.
J Ethnopharmacol. 2012 Feb 15; 139(3):772-9.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Campomanesia lineatifolia Ruiz and Pav. (Myrtaceae) is a native edible species found in the Amazon Rainforest, commonly known as gabiroba. In Brazil, Campomanesia species are frequently used in traditional medicine for gastrointestinal disorders.

MATERIALS AND METHODS

The present study performed phytochemical analyses and determined both the in vitro antioxidant activity of the ethanolic extract of Campomanesia lineatifolia leaves (EEC) as well as its ethyl acetate fraction (EAFC). In this analysis, quercetin was used as a positive control. Gastroprotective activity was also investigated at different oral doses in two experimental models in rats - gastric lesion induced by ethanol and gastric lesion induced by indomethacin. In this analysis, cimetidine and sucralfate were used as positive controls. The area of gastric lesion underwent macroscopic and histomorphometric evaluations, while the mucus content was estimated by applying the periodic acid-Schiff stain. Oral acute toxicity was also assessed.

RESULTS

Phytochemical studies revealed the presence of flavonoids and tannins. Catechin and quercitrin were isolated by bioguided chromatographic fractionation of EAFC. EEC and EAFC presented in vitro antioxidant activity. The oral administration of EEC and EAFC at doses 100-400 mg/kg (ethanol model) and at doses of 400-1200 mg/kg (indomethacin model) proved to be effective in preventing gastric ulcerations in rats. Pretreatment with EAFC (400mg/kg) significantly increased the gastric mucus content in the ethanol model. No animals died during the acute oral toxicology test.

CONCLUSIONS

Results confirm the Brazilian ethnopharmacological use of Campomanesia lineatifolia as a gastroprotective agent and indicate that the anti-ulcer effect is most likely mediated by scavenging free radicals due to the polyphenol content and, at least in part, by increasing the mucus secretion and the mucosal defense. In addition, EEC and EAFC were found to be safe when applied to a 2000 mg/kg single oral dose.

Authors+Show Affiliations

Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte-MG, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22178179

Citation

Madalosso, R C., et al. "Campomanesia Lineatifolia Ruiz & Pav. as a Gastroprotective Agent." Journal of Ethnopharmacology, vol. 139, no. 3, 2012, pp. 772-9.
Madalosso RC, Oliveira GC, Martins MT, et al. Campomanesia lineatifolia Ruiz & Pav. as a gastroprotective agent. J Ethnopharmacol. 2012;139(3):772-9.
Madalosso, R. C., Oliveira, G. C., Martins, M. T., Vieira, A. E., Barbosa, J., Caliari, M. V., Castilho, R. O., & Tagliati, C. A. (2012). Campomanesia lineatifolia Ruiz & Pav. as a gastroprotective agent. Journal of Ethnopharmacology, 139(3), 772-9. https://doi.org/10.1016/j.jep.2011.12.014
Madalosso RC, et al. Campomanesia Lineatifolia Ruiz & Pav. as a Gastroprotective Agent. J Ethnopharmacol. 2012 Feb 15;139(3):772-9. PubMed PMID: 22178179.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Campomanesia lineatifolia Ruiz & Pav. as a gastroprotective agent. AU - Madalosso,R C, AU - Oliveira,G C, AU - Martins,M T, AU - Vieira,A E D, AU - Barbosa,J, AU - Caliari,M V, AU - Castilho,R O, AU - Tagliati,C A, Y1 - 2011/12/13/ PY - 2011/08/18/received PY - 2011/10/27/revised PY - 2011/12/03/accepted PY - 2011/12/20/entrez PY - 2011/12/20/pubmed PY - 2012/6/14/medline SP - 772 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 139 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Campomanesia lineatifolia Ruiz and Pav. (Myrtaceae) is a native edible species found in the Amazon Rainforest, commonly known as gabiroba. In Brazil, Campomanesia species are frequently used in traditional medicine for gastrointestinal disorders. MATERIALS AND METHODS: The present study performed phytochemical analyses and determined both the in vitro antioxidant activity of the ethanolic extract of Campomanesia lineatifolia leaves (EEC) as well as its ethyl acetate fraction (EAFC). In this analysis, quercetin was used as a positive control. Gastroprotective activity was also investigated at different oral doses in two experimental models in rats - gastric lesion induced by ethanol and gastric lesion induced by indomethacin. In this analysis, cimetidine and sucralfate were used as positive controls. The area of gastric lesion underwent macroscopic and histomorphometric evaluations, while the mucus content was estimated by applying the periodic acid-Schiff stain. Oral acute toxicity was also assessed. RESULTS: Phytochemical studies revealed the presence of flavonoids and tannins. Catechin and quercitrin were isolated by bioguided chromatographic fractionation of EAFC. EEC and EAFC presented in vitro antioxidant activity. The oral administration of EEC and EAFC at doses 100-400 mg/kg (ethanol model) and at doses of 400-1200 mg/kg (indomethacin model) proved to be effective in preventing gastric ulcerations in rats. Pretreatment with EAFC (400mg/kg) significantly increased the gastric mucus content in the ethanol model. No animals died during the acute oral toxicology test. CONCLUSIONS: Results confirm the Brazilian ethnopharmacological use of Campomanesia lineatifolia as a gastroprotective agent and indicate that the anti-ulcer effect is most likely mediated by scavenging free radicals due to the polyphenol content and, at least in part, by increasing the mucus secretion and the mucosal defense. In addition, EEC and EAFC were found to be safe when applied to a 2000 mg/kg single oral dose. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22178179/Campomanesia_lineatifolia_Ruiz_&_Pav__as_a_gastroprotective_agent_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00881-6 DB - PRIME DP - Unbound Medicine ER -