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Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide.
Amino Acids. 2012 Sep; 43(3):1233-42.AA

Abstract

The neonatal small intestine is susceptible to damage by endotoxin, but effective methods for prevention and treatment are lacking. N-acetylcysteine (NAC) is a widely used precursor of L: -cysteine for animal cells and plays an important role in protecting cells against oxidative stress. This study was conducted with the lipopolysaccharide (LPS)-challenged piglet model to determine the effects of NAC on intestinal function. Eighteen piglets were randomly allocated into control, LPS and LPS + NAC groups. The control and LPS groups were fed a corn- and soybean meal-based diet, and the LPS + NAC group was fed the basal diet +500 mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS and LPS + NAC groups received intraperitoneal administration of LPS (100 μg/kg BW), whereas the control piglets received saline. On day 20 of the trial, D-: xylose (0.1 g/kg BW) was orally administrated to all piglets 2 h after LPS or saline injection, and blood samples were collected 1 h thereafter. One hour blood xylose test was used to measure intestinal absorption capacity and mucosal integrity, and diamine oxidase (DAO) was used as a marker of intestinal injury. On day 21 of the trial, pigs were killed to obtain the intestinal mucosa. Compared to the control, LPS challenge reduced (P < 0.05) the concentrations of D-: xylose (a marker of intestinal absorption) in plasma, activities of DAO in the jejunal mucosa, the ratio of villus height to crypt depth in the jejunal mucosa, RNA/DNA and protein/DNA in the jejunal and ileal mucosae, while increasing (P < 0.05) DAO activity in plasma and caspase-3 expression in the intestinal mucosa. The adverse effects of LPS were partially ameliorated (P < 0.05) by NAC supplementation. Moreover, NAC prevented the LPS-induced decrease in claudin-1 and occludin expression in the jejunal and ileal mucosae. Collectively, these results indicate that dietary NAC supplementation alleviates the mucosal damage and improves the absorptive function of the small intestine in LPS-challenged piglets.

Authors+Show Affiliations

Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China. houyq777@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22180025

Citation

Hou, Yongqing, et al. "Protective Effects of N-acetylcysteine On Intestinal Functions of Piglets Challenged With Lipopolysaccharide." Amino Acids, vol. 43, no. 3, 2012, pp. 1233-42.
Hou Y, Wang L, Zhang W, et al. Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide. Amino Acids. 2012;43(3):1233-42.
Hou, Y., Wang, L., Zhang, W., Yang, Z., Ding, B., Zhu, H., Liu, Y., Qiu, Y., Yin, Y., & Wu, G. (2012). Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide. Amino Acids, 43(3), 1233-42. https://doi.org/10.1007/s00726-011-1191-9
Hou Y, et al. Protective Effects of N-acetylcysteine On Intestinal Functions of Piglets Challenged With Lipopolysaccharide. Amino Acids. 2012;43(3):1233-42. PubMed PMID: 22180025.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide. AU - Hou,Yongqing, AU - Wang,Lei, AU - Zhang,Wei, AU - Yang,Zhenguo, AU - Ding,Binying, AU - Zhu,Huiling, AU - Liu,Yulan, AU - Qiu,Yinsheng, AU - Yin,Yulong, AU - Wu,Guoyao, Y1 - 2011/12/17/ PY - 2011/07/08/received PY - 2011/11/30/accepted PY - 2011/12/20/entrez PY - 2011/12/20/pubmed PY - 2012/12/22/medline SP - 1233 EP - 42 JF - Amino acids JO - Amino Acids VL - 43 IS - 3 N2 - The neonatal small intestine is susceptible to damage by endotoxin, but effective methods for prevention and treatment are lacking. N-acetylcysteine (NAC) is a widely used precursor of L: -cysteine for animal cells and plays an important role in protecting cells against oxidative stress. This study was conducted with the lipopolysaccharide (LPS)-challenged piglet model to determine the effects of NAC on intestinal function. Eighteen piglets were randomly allocated into control, LPS and LPS + NAC groups. The control and LPS groups were fed a corn- and soybean meal-based diet, and the LPS + NAC group was fed the basal diet +500 mg/kg NAC. On days 10, 13 and 20 of the trial, the LPS and LPS + NAC groups received intraperitoneal administration of LPS (100 μg/kg BW), whereas the control piglets received saline. On day 20 of the trial, D-: xylose (0.1 g/kg BW) was orally administrated to all piglets 2 h after LPS or saline injection, and blood samples were collected 1 h thereafter. One hour blood xylose test was used to measure intestinal absorption capacity and mucosal integrity, and diamine oxidase (DAO) was used as a marker of intestinal injury. On day 21 of the trial, pigs were killed to obtain the intestinal mucosa. Compared to the control, LPS challenge reduced (P < 0.05) the concentrations of D-: xylose (a marker of intestinal absorption) in plasma, activities of DAO in the jejunal mucosa, the ratio of villus height to crypt depth in the jejunal mucosa, RNA/DNA and protein/DNA in the jejunal and ileal mucosae, while increasing (P < 0.05) DAO activity in plasma and caspase-3 expression in the intestinal mucosa. The adverse effects of LPS were partially ameliorated (P < 0.05) by NAC supplementation. Moreover, NAC prevented the LPS-induced decrease in claudin-1 and occludin expression in the jejunal and ileal mucosae. Collectively, these results indicate that dietary NAC supplementation alleviates the mucosal damage and improves the absorptive function of the small intestine in LPS-challenged piglets. SN - 1438-2199 UR - https://www.unboundmedicine.com/medline/citation/22180025/Protective_effects_of_N_acetylcysteine_on_intestinal_functions_of_piglets_challenged_with_lipopolysaccharide_ L2 - https://dx.doi.org/10.1007/s00726-011-1191-9 DB - PRIME DP - Unbound Medicine ER -