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An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota.

Abstract

BACKGROUND AND AIMS

Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder that may be triggered by enteric pathogens and has also been linked to alterations in the microbiota and the host immune response. The authors performed a detailed analysis of the faecal microbiota in IBS and control subjects and correlated the findings with key clinical and physiological parameters.

DESIGN

The authors used pyrosequencing to determine faecal microbiota composition in 37 IBS patients (mean age 37 years; 26 female subjects; 15 diarrhoea-predominant IBS, 10 constipation-predominant IBS and 12 alternating-type IBS) and 20 age- and gender-matched controls. Gastrointestinal and psychological symptom severity and quality of life were evaluated with validated questionnaires and colonic transit time and rectal sensitivity were measured.

RESULTS

Associations detected between microbiota composition and clinical or physiological phenotypes included microbial signatures associated with colonic transit and levels of clinically significant depression in the disease. Clustering by microbiota composition revealed subgroups of IBS patients, one of which (n=15) showed normal-like microbiota composition compared with healthy controls. The other IBS samples (n=22) were defined by large microbiota-wide changes characterised by an increase of Firmicutes-associated taxa and a depletion of Bacteroidetes-related taxa.

CONCLUSIONS

Detailed microbiota analysis of a well-characterised cohort of IBS patients identified several clear associations with clinical data and a distinct subset of IBS patients with alterations in their microbiota that did not correspond to IBS subtypes, as defined by the Rome II criteria.

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  • Authors+Show Affiliations

    ,

    Department of Microbiology, University College Cork, Cork, Ireland.

    , , , , ,

    Source

    Gut 61:7 2012 Jul pg 997-1006

    MeSH

    Adult
    Bacteroidetes
    Case-Control Studies
    Clostridium
    Feces
    Female
    Genes, rRNA
    Gram-Positive Bacteria
    Humans
    Irritable Bowel Syndrome
    Male
    Metagenome
    Quality of Life
    Surveys and Questionnaires

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22180058

    Citation

    Jeffery, Ian B., et al. "An Irritable Bowel Syndrome Subtype Defined By Species-specific Alterations in Faecal Microbiota." Gut, vol. 61, no. 7, 2012, pp. 997-1006.
    Jeffery IB, O'Toole PW, Öhman L, et al. An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota. Gut. 2012;61(7):997-1006.
    Jeffery, I. B., O'Toole, P. W., Öhman, L., Claesson, M. J., Deane, J., Quigley, E. M., & Simrén, M. (2012). An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota. Gut, 61(7), pp. 997-1006. doi:10.1136/gutjnl-2011-301501.
    Jeffery IB, et al. An Irritable Bowel Syndrome Subtype Defined By Species-specific Alterations in Faecal Microbiota. Gut. 2012;61(7):997-1006. PubMed PMID: 22180058.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota. AU - Jeffery,Ian B, AU - O'Toole,Paul W, AU - Öhman,Lena, AU - Claesson,Marcus J, AU - Deane,Jennifer, AU - Quigley,Eamonn M M, AU - Simrén,Magnus, Y1 - 2011/12/16/ PY - 2011/12/20/entrez PY - 2011/12/20/pubmed PY - 2012/10/10/medline SP - 997 EP - 1006 JF - Gut JO - Gut VL - 61 IS - 7 N2 - BACKGROUND AND AIMS: Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder that may be triggered by enteric pathogens and has also been linked to alterations in the microbiota and the host immune response. The authors performed a detailed analysis of the faecal microbiota in IBS and control subjects and correlated the findings with key clinical and physiological parameters. DESIGN: The authors used pyrosequencing to determine faecal microbiota composition in 37 IBS patients (mean age 37 years; 26 female subjects; 15 diarrhoea-predominant IBS, 10 constipation-predominant IBS and 12 alternating-type IBS) and 20 age- and gender-matched controls. Gastrointestinal and psychological symptom severity and quality of life were evaluated with validated questionnaires and colonic transit time and rectal sensitivity were measured. RESULTS: Associations detected between microbiota composition and clinical or physiological phenotypes included microbial signatures associated with colonic transit and levels of clinically significant depression in the disease. Clustering by microbiota composition revealed subgroups of IBS patients, one of which (n=15) showed normal-like microbiota composition compared with healthy controls. The other IBS samples (n=22) were defined by large microbiota-wide changes characterised by an increase of Firmicutes-associated taxa and a depletion of Bacteroidetes-related taxa. CONCLUSIONS: Detailed microbiota analysis of a well-characterised cohort of IBS patients identified several clear associations with clinical data and a distinct subset of IBS patients with alterations in their microbiota that did not correspond to IBS subtypes, as defined by the Rome II criteria. SN - 1468-3288 UR - https://www.unboundmedicine.com/medline/citation/22180058/An_irritable_bowel_syndrome_subtype_defined_by_species_specific_alterations_in_faecal_microbiota_ L2 - http://gut.bmj.com/cgi/pmidlookup?view=long&pmid=22180058 DB - PRIME DP - Unbound Medicine ER -