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Systemic CD4+ and CD8+ T-cell cytokine profiles correlate with GOLD stage in stable COPD.
Eur Respir J. 2012 Aug; 40(2):330-7.ER

Abstract

Chronic obstructive pulmonary disease (COPD) is associated with pulmonary and systemic inflammation. Both CD4+ and CD8+ T-lymphocytes play a key role in COPD pathogenesis, but cytokine profiles in circulating T-lymphocytes have not been well characterised. Here we report the analysis of peripheral blood T-cells from 30 stable COPD patients and 10 healthy never-smokers for interferon (IFN)-γ, interleukin (IL)-4, tumour necrosis factor (TNF)-α and the T-helper 17 cytokines IL-17A, IL-17F and IL-22 by intracellular flow cytometry. We found significantly increased proportions of IFN-γ+ and TNF-α+ CD8+ T-cells in COPD patients, when compared with healthy controls. This was most evident in patients with less severe disease. In contrast, expression profiles in circulating CD4+ T-cells were similar in COPD patients and healthy controls for all cytokines tested, except for IL-17F. COPD patients with more severely reduced diffusing capacity had lower proportions of IL-17A+ CD4+ T-cells. Proportions of IL-22+ cells in the CD4+ memory T-cell population were significantly increased in active smokers, when compared with past smokers. Collectively, this comprehensive cytokine analysis of circulating T-cells in COPD patients revealed a correlation for CD8+ T-cells between Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage and IFN-γ or TNF-α expression, but not for CD4+ T-cells.

Authors+Show Affiliations

Dept of Pulmonary Medicine, Erasmus MC Rotterdam, PO Box 2040, NL 3000 CA Rotterdam, The Netherlands. m.paats@erasmusmc.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22183488

Citation

Paats, Marthe S., et al. "Systemic CD4+ and CD8+ T-cell Cytokine Profiles Correlate With GOLD Stage in Stable COPD." The European Respiratory Journal, vol. 40, no. 2, 2012, pp. 330-7.
Paats MS, Bergen IM, Hoogsteden HC, et al. Systemic CD4+ and CD8+ T-cell cytokine profiles correlate with GOLD stage in stable COPD. Eur Respir J. 2012;40(2):330-7.
Paats, M. S., Bergen, I. M., Hoogsteden, H. C., van der Eerden, M. M., & Hendriks, R. W. (2012). Systemic CD4+ and CD8+ T-cell cytokine profiles correlate with GOLD stage in stable COPD. The European Respiratory Journal, 40(2), 330-7. https://doi.org/10.1183/09031936.00079611
Paats MS, et al. Systemic CD4+ and CD8+ T-cell Cytokine Profiles Correlate With GOLD Stage in Stable COPD. Eur Respir J. 2012;40(2):330-7. PubMed PMID: 22183488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Systemic CD4+ and CD8+ T-cell cytokine profiles correlate with GOLD stage in stable COPD. AU - Paats,Marthe S, AU - Bergen,Ingrid M, AU - Hoogsteden,Henk C, AU - van der Eerden,Menno M, AU - Hendriks,Rudi W, Y1 - 2011/12/19/ PY - 2011/12/21/entrez PY - 2011/12/21/pubmed PY - 2013/1/11/medline SP - 330 EP - 7 JF - The European respiratory journal JO - Eur Respir J VL - 40 IS - 2 N2 - Chronic obstructive pulmonary disease (COPD) is associated with pulmonary and systemic inflammation. Both CD4+ and CD8+ T-lymphocytes play a key role in COPD pathogenesis, but cytokine profiles in circulating T-lymphocytes have not been well characterised. Here we report the analysis of peripheral blood T-cells from 30 stable COPD patients and 10 healthy never-smokers for interferon (IFN)-γ, interleukin (IL)-4, tumour necrosis factor (TNF)-α and the T-helper 17 cytokines IL-17A, IL-17F and IL-22 by intracellular flow cytometry. We found significantly increased proportions of IFN-γ+ and TNF-α+ CD8+ T-cells in COPD patients, when compared with healthy controls. This was most evident in patients with less severe disease. In contrast, expression profiles in circulating CD4+ T-cells were similar in COPD patients and healthy controls for all cytokines tested, except for IL-17F. COPD patients with more severely reduced diffusing capacity had lower proportions of IL-17A+ CD4+ T-cells. Proportions of IL-22+ cells in the CD4+ memory T-cell population were significantly increased in active smokers, when compared with past smokers. Collectively, this comprehensive cytokine analysis of circulating T-cells in COPD patients revealed a correlation for CD8+ T-cells between Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage and IFN-γ or TNF-α expression, but not for CD4+ T-cells. SN - 1399-3003 UR - https://www.unboundmedicine.com/medline/citation/22183488/Systemic_CD4+_and_CD8+_T_cell_cytokine_profiles_correlate_with_GOLD_stage_in_stable_COPD_ L2 - http://erj.ersjournals.com/cgi/pmidlookup?view=long&pmid=22183488 DB - PRIME DP - Unbound Medicine ER -