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Development and validation of a highly sensitive UPLC-MS/MS method for simultaneous determination of aconitine, mesaconitine, hypaconitine, and five of their metabolites in rat blood and its application to a pharmacokinetics study of aconitine, mesaconitine, and hypaconitine.
Xenobiotica 2012; 42(6):518-25X

Abstract

A rapid, specific and sensitive method was developed for the simultaneous determination of eight Aconitum alkaloids: aconitine (AC), mesaconitine (MA), hypaconitine (HA), benzoylaconine (BAC), benzoylmesaconine (BMA), benzoylhypaconine (BHA), aconine and mesaconine in rat blood by ultra-performance liquid chromatography coupled tandem mass spectrometry (UPLC-MS/MS). The UPLC-MS/MS system coupled with an electrospray ionization (ESI) source was operated in a positive mode via multiple-reaction monitoring (MRM). Samples were treated with methanol to remove protein prior to analysis by UPLC-MS/MS. The analytes were separated with a Waters C18 column (1.7 µm, 50 × 2.1 mm) and a gradient elution using acetonitrile and 0.1% formic acid-water as the mobile phases. The linear response range was from 0.125 to 1000 nmol/L for these eight alkaloids and the correlation coefficients (r(2) values) were all higher than 0.997. The method was validated with respect to precision, accuracy, recovery, matrix effect, carryover effect and sample stability, and found to be within the acceptable limits. The developed and validated method was successfully applied to simultaneously determine the eight Aconitum alkaloids in rats blood after intravenous administration of a mixture of AC, MA and HA.

Authors+Show Affiliations

Department of Pharmaceutics, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

22188409

Citation

Ye, Ling, et al. "Development and Validation of a Highly Sensitive UPLC-MS/MS Method for Simultaneous Determination of Aconitine, Mesaconitine, Hypaconitine, and Five of Their Metabolites in Rat Blood and Its Application to a Pharmacokinetics Study of Aconitine, Mesaconitine, and Hypaconitine." Xenobiotica; the Fate of Foreign Compounds in Biological Systems, vol. 42, no. 6, 2012, pp. 518-25.
Ye L, Gao S, Feng Q, et al. Development and validation of a highly sensitive UPLC-MS/MS method for simultaneous determination of aconitine, mesaconitine, hypaconitine, and five of their metabolites in rat blood and its application to a pharmacokinetics study of aconitine, mesaconitine, and hypaconitine. Xenobiotica. 2012;42(6):518-25.
Ye, L., Gao, S., Feng, Q., Liu, W., Yang, Z., Hu, M., & Liu, Z. (2012). Development and validation of a highly sensitive UPLC-MS/MS method for simultaneous determination of aconitine, mesaconitine, hypaconitine, and five of their metabolites in rat blood and its application to a pharmacokinetics study of aconitine, mesaconitine, and hypaconitine. Xenobiotica; the Fate of Foreign Compounds in Biological Systems, 42(6), pp. 518-25. doi:10.3109/00498254.2011.641608.
Ye L, et al. Development and Validation of a Highly Sensitive UPLC-MS/MS Method for Simultaneous Determination of Aconitine, Mesaconitine, Hypaconitine, and Five of Their Metabolites in Rat Blood and Its Application to a Pharmacokinetics Study of Aconitine, Mesaconitine, and Hypaconitine. Xenobiotica. 2012;42(6):518-25. PubMed PMID: 22188409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a highly sensitive UPLC-MS/MS method for simultaneous determination of aconitine, mesaconitine, hypaconitine, and five of their metabolites in rat blood and its application to a pharmacokinetics study of aconitine, mesaconitine, and hypaconitine. AU - Ye,Ling, AU - Gao,Song, AU - Feng,Qian, AU - Liu,Wei, AU - Yang,Zhen, AU - Hu,Ming, AU - Liu,Zhongqiu, Y1 - 2011/12/22/ PY - 2011/12/23/entrez PY - 2011/12/23/pubmed PY - 2012/8/28/medline SP - 518 EP - 25 JF - Xenobiotica; the fate of foreign compounds in biological systems JO - Xenobiotica VL - 42 IS - 6 N2 - A rapid, specific and sensitive method was developed for the simultaneous determination of eight Aconitum alkaloids: aconitine (AC), mesaconitine (MA), hypaconitine (HA), benzoylaconine (BAC), benzoylmesaconine (BMA), benzoylhypaconine (BHA), aconine and mesaconine in rat blood by ultra-performance liquid chromatography coupled tandem mass spectrometry (UPLC-MS/MS). The UPLC-MS/MS system coupled with an electrospray ionization (ESI) source was operated in a positive mode via multiple-reaction monitoring (MRM). Samples were treated with methanol to remove protein prior to analysis by UPLC-MS/MS. The analytes were separated with a Waters C18 column (1.7 µm, 50 × 2.1 mm) and a gradient elution using acetonitrile and 0.1% formic acid-water as the mobile phases. The linear response range was from 0.125 to 1000 nmol/L for these eight alkaloids and the correlation coefficients (r(2) values) were all higher than 0.997. The method was validated with respect to precision, accuracy, recovery, matrix effect, carryover effect and sample stability, and found to be within the acceptable limits. The developed and validated method was successfully applied to simultaneously determine the eight Aconitum alkaloids in rats blood after intravenous administration of a mixture of AC, MA and HA. SN - 1366-5928 UR - https://www.unboundmedicine.com/medline/citation/22188409/Development_and_validation_of_a_highly_sensitive_UPLC_MS/MS_method_for_simultaneous_determination_of_aconitine_mesaconitine_hypaconitine_and_five_of_their_metabolites_in_rat_blood_and_its_application_to_a_pharmacokinetics_study_of_aconitine_mesaconitine_and_hypaconitine_ L2 - http://www.tandfonline.com/doi/full/10.3109/00498254.2011.641608 DB - PRIME DP - Unbound Medicine ER -