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bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice.
Planta. 2012 Jun; 235(6):1157-69.P

Abstract

OsbZIP52/RISBZ5 is a member of the basic leucine zipper (bZIP) transcription factor (TF) family in rice (Oryza sativa) isolated from rice (Zhonghua11) panicles. Expression of the OsbZIP52 gene was strongly induced by low temperature (4°C) but not by drought, PEG, salt, or ABA. The subcellular localization of OsbZIP52-GFP in onion (Allium cepa) epidermis cells revealed that OsbZIP52 is a nuclear localized protein. A transactivation assay in yeast demonstrated that OsbZIP52 functions as a transcriptional activator and can specifically bind to the G-box promoter motif. In a yeast two-hybrid (Y-2-H) experiment, OsbZIP52 was able to form homodimeric complexes. Rice plants overexpressing OsbZIP52 showed significantly increased sensitivity to cold and drought stress. Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, β-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines. These results suggest that OsbZIP52/RISBZ5 could function as a negative regulator in cold and drought stress environments.

Authors+Show Affiliations

Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22189955

Citation

Liu, Citao, et al. "BZIP Transcription Factor OsbZIP52/RISBZ5: a Potential Negative Regulator of Cold and Drought Stress Response in Rice." Planta, vol. 235, no. 6, 2012, pp. 1157-69.
Liu C, Wu Y, Wang X. BZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice. Planta. 2012;235(6):1157-69.
Liu, C., Wu, Y., & Wang, X. (2012). BZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice. Planta, 235(6), 1157-69. https://doi.org/10.1007/s00425-011-1564-z
Liu C, Wu Y, Wang X. BZIP Transcription Factor OsbZIP52/RISBZ5: a Potential Negative Regulator of Cold and Drought Stress Response in Rice. Planta. 2012;235(6):1157-69. PubMed PMID: 22189955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice. AU - Liu,Citao, AU - Wu,Yanbin, AU - Wang,Xiping, Y1 - 2011/12/22/ PY - 2011/08/31/received PY - 2011/11/28/accepted PY - 2011/12/23/entrez PY - 2011/12/23/pubmed PY - 2012/9/25/medline SP - 1157 EP - 69 JF - Planta JO - Planta VL - 235 IS - 6 N2 - OsbZIP52/RISBZ5 is a member of the basic leucine zipper (bZIP) transcription factor (TF) family in rice (Oryza sativa) isolated from rice (Zhonghua11) panicles. Expression of the OsbZIP52 gene was strongly induced by low temperature (4°C) but not by drought, PEG, salt, or ABA. The subcellular localization of OsbZIP52-GFP in onion (Allium cepa) epidermis cells revealed that OsbZIP52 is a nuclear localized protein. A transactivation assay in yeast demonstrated that OsbZIP52 functions as a transcriptional activator and can specifically bind to the G-box promoter motif. In a yeast two-hybrid (Y-2-H) experiment, OsbZIP52 was able to form homodimeric complexes. Rice plants overexpressing OsbZIP52 showed significantly increased sensitivity to cold and drought stress. Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, β-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines. These results suggest that OsbZIP52/RISBZ5 could function as a negative regulator in cold and drought stress environments. SN - 1432-2048 UR - https://www.unboundmedicine.com/medline/citation/22189955/bZIP_transcription_factor_OsbZIP52/RISBZ5:_a_potential_negative_regulator_of_cold_and_drought_stress_response_in_rice_ L2 - https://dx.doi.org/10.1007/s00425-011-1564-z DB - PRIME DP - Unbound Medicine ER -