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Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity.
Environ Toxicol Pharmacol 2012; 33(1):107-19ET

Abstract

Present study was designed to compare cytoprotective and antigenotoxic activity of the polyphenolic fraction of Pilea microphylla (PM1) with that of its active polyphenolic constituents against γ-radiation in V79 cells. PM1 was standardized with respect to the polyphenols present by RP-HPLC. It was evaluated for its free radical scavenging potential using Fenton reaction-induced DNA damage and lipid peroxidation. Further, PM1 was subjected against γ-radiation-induced cytotoxicity and genotoxicity in V79 cells. PM1 significantly reduced free radical-mediated calf thymus DNA damage and lipid peroxidation. Among the concentrations tested (12.5, 25 and 50 μg/ml) for radioprotection, PM1 at 25 μg/ml exhibited maximum protection. Further, when compared with constituent polyphenols viz., rutin, quercetin and chlorogenic acid (concentrations equivalent to that present in PM1-25 μg/ml), a combination of polyphenols was found most effective in preventing γ-radiation-induced cytotoxicity and genotoxicity. To conclude, radioprotection is possibly a synergistic effect of the phytochemicals present in the herbal extract, rather than any single component.

Authors+Show Affiliations

Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576104, Karnataka, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22196050

Citation

Paul, Piya, et al. "Polyphenolic Fraction of Pilea Microphylla (L.) Protects Chinese Hamster Lung Fibroblasts Against Γ-radiation-induced Cytotoxicity and Genotoxicity." Environmental Toxicology and Pharmacology, vol. 33, no. 1, 2012, pp. 107-19.
Paul P, Bansal P, Nayak PG, et al. Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity. Environ Toxicol Pharmacol. 2012;33(1):107-19.
Paul, P., Bansal, P., Nayak, P. G., Pannakal, S. T., Priyadarsini, K. I., & Unnikrishnan, M. K. (2012). Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity. Environmental Toxicology and Pharmacology, 33(1), pp. 107-19. doi:10.1016/j.etap.2011.09.007.
Paul P, et al. Polyphenolic Fraction of Pilea Microphylla (L.) Protects Chinese Hamster Lung Fibroblasts Against Γ-radiation-induced Cytotoxicity and Genotoxicity. Environ Toxicol Pharmacol. 2012;33(1):107-19. PubMed PMID: 22196050.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against γ-radiation-induced cytotoxicity and genotoxicity. AU - Paul,Piya, AU - Bansal,Punit, AU - Nayak,Pawan G, AU - Pannakal,Steve Thomas, AU - Priyadarsini,K I, AU - Unnikrishnan,M K, Y1 - 2011/09/24/ PY - 2011/05/11/received PY - 2011/09/15/revised PY - 2011/09/16/accepted PY - 2011/12/27/entrez PY - 2011/12/27/pubmed PY - 2012/4/17/medline SP - 107 EP - 19 JF - Environmental toxicology and pharmacology JO - Environ. Toxicol. Pharmacol. VL - 33 IS - 1 N2 - Present study was designed to compare cytoprotective and antigenotoxic activity of the polyphenolic fraction of Pilea microphylla (PM1) with that of its active polyphenolic constituents against γ-radiation in V79 cells. PM1 was standardized with respect to the polyphenols present by RP-HPLC. It was evaluated for its free radical scavenging potential using Fenton reaction-induced DNA damage and lipid peroxidation. Further, PM1 was subjected against γ-radiation-induced cytotoxicity and genotoxicity in V79 cells. PM1 significantly reduced free radical-mediated calf thymus DNA damage and lipid peroxidation. Among the concentrations tested (12.5, 25 and 50 μg/ml) for radioprotection, PM1 at 25 μg/ml exhibited maximum protection. Further, when compared with constituent polyphenols viz., rutin, quercetin and chlorogenic acid (concentrations equivalent to that present in PM1-25 μg/ml), a combination of polyphenols was found most effective in preventing γ-radiation-induced cytotoxicity and genotoxicity. To conclude, radioprotection is possibly a synergistic effect of the phytochemicals present in the herbal extract, rather than any single component. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/22196050/Polyphenolic_fraction_of_Pilea_microphylla__L___protects_Chinese_hamster_lung_fibroblasts_against_γ_radiation_induced_cytotoxicity_and_genotoxicity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1382-6689(11)00146-3 DB - PRIME DP - Unbound Medicine ER -