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Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings.
J Plant Physiol. 2012 Mar 15; 169(5):478-86.JP

Abstract

Hydrogen peroxide (H2O2) is considered a signal molecule inducing cellular stress. Both heat shock (HS) and Cd can increase H2O2 content. We investigated the involvement of H2O2 in HS- and Cd-mediated changes in the expression of ascorbate peroxidase (APX) and glutathione reductase (GR) in leaves of rice seedlings. HS treatment increased the content of H2O2 before it increased activities of APX and GR in rice leaves. Moreover, HS-induced H2O2 production and APX and GR activities could be counteracted by the NADPH oxidase inhibitors dipehenylene iodonium (DPI) and imidazole (IMD). HS-induced OsAPX2 gene expression was associated with HS-induced APX activity but was not regulated by H2O2. Cd-increased H2O2 content and APX and GR activities were lower with than without HS. Cd did not increase the expression of OsAPX and OsGR without HS treatment. Cd increased H2O2 content by Cd before it increased APX and GR activities without HS. Treatment with DPI and IMD effectively inhibited Cd-induced H2O2 production and APX and GR activities. Moreover, the effects of DPI and IMD could be rescued with H2O2 treatment. H2O2 may be involved in the regulation of HS- and Cd-increased APX and GR activities in leaves of rice seedlings.

Authors+Show Affiliations

Department of Agronomy, National Taiwan University, Taipei, Taiwan, ROC.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22196946

Citation

Chou, Ting-Shao, et al. "Involvement of Hydrogen Peroxide in Heat Shock- and Cadmium-induced Expression of Ascorbate Peroxidase and Glutathione Reductase in Leaves of Rice Seedlings." Journal of Plant Physiology, vol. 169, no. 5, 2012, pp. 478-86.
Chou TS, Chao YY, Kao CH. Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. J Plant Physiol. 2012;169(5):478-86.
Chou, T. S., Chao, Y. Y., & Kao, C. H. (2012). Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. Journal of Plant Physiology, 169(5), 478-86. https://doi.org/10.1016/j.jplph.2011.11.012
Chou TS, Chao YY, Kao CH. Involvement of Hydrogen Peroxide in Heat Shock- and Cadmium-induced Expression of Ascorbate Peroxidase and Glutathione Reductase in Leaves of Rice Seedlings. J Plant Physiol. 2012 Mar 15;169(5):478-86. PubMed PMID: 22196946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings. AU - Chou,Ting-Shao, AU - Chao,Yun-Yang, AU - Kao,Ching Huei, Y1 - 2011/12/22/ PY - 2011/09/09/received PY - 2011/11/28/revised PY - 2011/11/30/accepted PY - 2011/12/27/entrez PY - 2011/12/27/pubmed PY - 2012/8/16/medline SP - 478 EP - 86 JF - Journal of plant physiology JO - J Plant Physiol VL - 169 IS - 5 N2 - Hydrogen peroxide (H2O2) is considered a signal molecule inducing cellular stress. Both heat shock (HS) and Cd can increase H2O2 content. We investigated the involvement of H2O2 in HS- and Cd-mediated changes in the expression of ascorbate peroxidase (APX) and glutathione reductase (GR) in leaves of rice seedlings. HS treatment increased the content of H2O2 before it increased activities of APX and GR in rice leaves. Moreover, HS-induced H2O2 production and APX and GR activities could be counteracted by the NADPH oxidase inhibitors dipehenylene iodonium (DPI) and imidazole (IMD). HS-induced OsAPX2 gene expression was associated with HS-induced APX activity but was not regulated by H2O2. Cd-increased H2O2 content and APX and GR activities were lower with than without HS. Cd did not increase the expression of OsAPX and OsGR without HS treatment. Cd increased H2O2 content by Cd before it increased APX and GR activities without HS. Treatment with DPI and IMD effectively inhibited Cd-induced H2O2 production and APX and GR activities. Moreover, the effects of DPI and IMD could be rescued with H2O2 treatment. H2O2 may be involved in the regulation of HS- and Cd-increased APX and GR activities in leaves of rice seedlings. SN - 1618-1328 UR - https://www.unboundmedicine.com/medline/citation/22196946/Involvement_of_hydrogen_peroxide_in_heat_shock__and_cadmium_induced_expression_of_ascorbate_peroxidase_and_glutathione_reductase_in_leaves_of_rice_seedlings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0176-1617(11)00488-3 DB - PRIME DP - Unbound Medicine ER -