Tags

Type your tag names separated by a space and hit enter

Three-phase hollow fiber microextraction based on two immiscible organic solvents for determination of tricyclic antidepressant drugs: comparison with conventional three-phase hollow fiber microextraction.
J Chromatogr A. 2012 Jan 27; 1222:5-12.JC

Abstract

The aim of this research was to compare the extraction efficiencies of two modes of three-phase hollow fiber microextraction (HF-LLLME) based on aqueous and organic acceptor phases for analysis of tricyclic antidepressant (TCA) drugs. High-performance liquid chromatography with photodiode array detection (HPLC-DAD) was applied for determination of the drugs. In order to examine the ability of the new concept of HF-LLLME based on organic acceptor solvent in comparison with aqueous acceptor phase to extract the analytes, four TCAs were selected. The effect of different extraction conditions (i.e., type of acceptor phase, hollow fiber length, ionic strength, stirring rate, and extraction time) on the extraction efficiency of the TCAs was investigated and optimized using central composite design (CCD) as a powerful tool. Both methods were characterized by good linearity and high repeatability, but HF-LLLME with organic acceptor provided higher extraction efficiency and thus lower limits of detection (LODs). Calibration curves were linear (r(2)>0.996) in the range of 0.2-200 μgL(-1). LODs for all the TCAs ranged from 0.08 to 0.2 μgL(-1) using HPLC-DAD. Also an improvement in sensitivity of several orders of magnitude was achieved using single-ion monitoring GC-MS analyses (0.04 μgL(-1)) due to compatibility of this technique with GC instrument. The applicability of the proposed HF-LLLME/GC-MS and HPLC-DAD methods was demonstrated by analyzing the drugs in spiked urine and plasma samples. The obtained recoveries of the drugs in the range of 87.9-109.2% indicated the excellent capability of the developed method for extraction of TCAs from complex matrices.

Authors+Show Affiliations

Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, PO Box: 14115-175, Tehran, Iran.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22197253

Citation

Ghambarian, Mahnaz, et al. "Three-phase Hollow Fiber Microextraction Based On Two Immiscible Organic Solvents for Determination of Tricyclic Antidepressant Drugs: Comparison With Conventional Three-phase Hollow Fiber Microextraction." Journal of Chromatography. A, vol. 1222, 2012, pp. 5-12.
Ghambarian M, Yamini Y, Esrafili A. Three-phase hollow fiber microextraction based on two immiscible organic solvents for determination of tricyclic antidepressant drugs: comparison with conventional three-phase hollow fiber microextraction. J Chromatogr A. 2012;1222:5-12.
Ghambarian, M., Yamini, Y., & Esrafili, A. (2012). Three-phase hollow fiber microextraction based on two immiscible organic solvents for determination of tricyclic antidepressant drugs: comparison with conventional three-phase hollow fiber microextraction. Journal of Chromatography. A, 1222, 5-12. https://doi.org/10.1016/j.chroma.2011.11.055
Ghambarian M, Yamini Y, Esrafili A. Three-phase Hollow Fiber Microextraction Based On Two Immiscible Organic Solvents for Determination of Tricyclic Antidepressant Drugs: Comparison With Conventional Three-phase Hollow Fiber Microextraction. J Chromatogr A. 2012 Jan 27;1222:5-12. PubMed PMID: 22197253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Three-phase hollow fiber microextraction based on two immiscible organic solvents for determination of tricyclic antidepressant drugs: comparison with conventional three-phase hollow fiber microextraction. AU - Ghambarian,Mahnaz, AU - Yamini,Yadollah, AU - Esrafili,Ali, Y1 - 2011/12/06/ PY - 2011/09/27/received PY - 2011/11/19/revised PY - 2011/11/22/accepted PY - 2011/12/27/entrez PY - 2011/12/27/pubmed PY - 2012/3/2/medline SP - 5 EP - 12 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1222 N2 - The aim of this research was to compare the extraction efficiencies of two modes of three-phase hollow fiber microextraction (HF-LLLME) based on aqueous and organic acceptor phases for analysis of tricyclic antidepressant (TCA) drugs. High-performance liquid chromatography with photodiode array detection (HPLC-DAD) was applied for determination of the drugs. In order to examine the ability of the new concept of HF-LLLME based on organic acceptor solvent in comparison with aqueous acceptor phase to extract the analytes, four TCAs were selected. The effect of different extraction conditions (i.e., type of acceptor phase, hollow fiber length, ionic strength, stirring rate, and extraction time) on the extraction efficiency of the TCAs was investigated and optimized using central composite design (CCD) as a powerful tool. Both methods were characterized by good linearity and high repeatability, but HF-LLLME with organic acceptor provided higher extraction efficiency and thus lower limits of detection (LODs). Calibration curves were linear (r(2)>0.996) in the range of 0.2-200 μgL(-1). LODs for all the TCAs ranged from 0.08 to 0.2 μgL(-1) using HPLC-DAD. Also an improvement in sensitivity of several orders of magnitude was achieved using single-ion monitoring GC-MS analyses (0.04 μgL(-1)) due to compatibility of this technique with GC instrument. The applicability of the proposed HF-LLLME/GC-MS and HPLC-DAD methods was demonstrated by analyzing the drugs in spiked urine and plasma samples. The obtained recoveries of the drugs in the range of 87.9-109.2% indicated the excellent capability of the developed method for extraction of TCAs from complex matrices. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/22197253/Three_phase_hollow_fiber_microextraction_based_on_two_immiscible_organic_solvents_for_determination_of_tricyclic_antidepressant_drugs:_comparison_with_conventional_three_phase_hollow_fiber_microextraction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(11)01771-7 DB - PRIME DP - Unbound Medicine ER -