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A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jan 15; 881-882:83-9.JC

Abstract

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for simultaneous determination of five acid/alkaline phytohormones, i.e., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthylacetic acid (NAA), gibberellic acid (GA(3)) and isopentenyladenine (2IP), in grapes was developed. After optimization, the samples were extracted with methanol containing 1% formic acid and purified by Oasis HLB SPE cartridges. The analytes were separated on a Thermo Hypersil Gold column (100 mm×2.1 mm, 3.0 μm) with water and acetonitrile, then determined with Thermo tandem quadrupole mass spectrometer operating in negative electro-spray ionization using selected reaction monitoring (SRM) mode. The established method was further validated by determining the linearity (R² ≥ 0.9990), average recovery (82.5-105.4%), sensitivity (0.05-1.00 ng mL⁻¹), precision (RSD ≤1 3.0%) and stability (RSD ≥ 82.0%). Finally, the application of the approach proposed to thirty grape samples convinced its desirable performance for rapid analysis of multiclass phytohormones, supporting its sufficient capability for multiresidue analyses or other analytical system targeting phytohormones in agriculture field.

Authors+Show Affiliations

Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1018 Jinqi Road, Shanghai 201403, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22197443

Citation

Han, Zheng, et al. "A Liquid Chromatography Tandem Mass Spectrometry Method for Simultaneous Determination of Acid/alkaline Phytohormones in Grapes." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 881-882, 2012, pp. 83-9.
Han Z, Liu G, Rao Q, et al. A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;881-882:83-9.
Han, Z., Liu, G., Rao, Q., Bai, B., Zhao, Z., Liu, H., & Wu, A. (2012). A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 881-882, 83-9. https://doi.org/10.1016/j.jchromb.2011.12.002
Han Z, et al. A Liquid Chromatography Tandem Mass Spectrometry Method for Simultaneous Determination of Acid/alkaline Phytohormones in Grapes. J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jan 15;881-882:83-9. PubMed PMID: 22197443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes. AU - Han,Zheng, AU - Liu,Gang, AU - Rao,Qinxiong, AU - Bai,Bing, AU - Zhao,Zhihui, AU - Liu,Hong, AU - Wu,Aibo, Y1 - 2011/12/10/ PY - 2011/10/12/received PY - 2011/12/01/revised PY - 2011/12/02/accepted PY - 2011/12/27/entrez PY - 2011/12/27/pubmed PY - 2012/5/1/medline SP - 83 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 881-882 N2 - A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for simultaneous determination of five acid/alkaline phytohormones, i.e., indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthylacetic acid (NAA), gibberellic acid (GA(3)) and isopentenyladenine (2IP), in grapes was developed. After optimization, the samples were extracted with methanol containing 1% formic acid and purified by Oasis HLB SPE cartridges. The analytes were separated on a Thermo Hypersil Gold column (100 mm×2.1 mm, 3.0 μm) with water and acetonitrile, then determined with Thermo tandem quadrupole mass spectrometer operating in negative electro-spray ionization using selected reaction monitoring (SRM) mode. The established method was further validated by determining the linearity (R² ≥ 0.9990), average recovery (82.5-105.4%), sensitivity (0.05-1.00 ng mL⁻¹), precision (RSD ≤1 3.0%) and stability (RSD ≥ 82.0%). Finally, the application of the approach proposed to thirty grape samples convinced its desirable performance for rapid analysis of multiclass phytohormones, supporting its sufficient capability for multiresidue analyses or other analytical system targeting phytohormones in agriculture field. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/22197443/A_liquid_chromatography_tandem_mass_spectrometry_method_for_simultaneous_determination_of_acid/alkaline_phytohormones_in_grapes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(11)00789-6 DB - PRIME DP - Unbound Medicine ER -