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Relationship between thiol, tyrosine nitration and carbonyl formation as biomarkers of oxidative stress and changes of hemostatic function of plasma from breast cancer patients before surgery.
Clin Biochem 2012; 45(3):231-6CB

Abstract

OBJECTIVES

In breast cancer patients dysregulation of reactive oxygen species metabolism, as detected by various indicators in plasma or various blood cells, including red blood cells and blood platelets, is observed.

DESIGN AND METHODS

The aim of our study was to explain the effect of oxidative/nitrative stress on hemostatic activity of plasma from breast cancer patients. Different methods were used to analyze oxidative/nitrative stress (carbonyl groups and 3-nitrotyrosine levels - ELISA and C-ELISA method, respectively and free thiol groups was estimated with 5,5'-dithio-bis(2-nitro-benzoic acid)). We also measured total antioxidant status (TAS) in plasma.

RESULTS

Our results showed the correlation between the oxidative stress and changes of hemostasis in breast cancer patients; the correlation between the increased amount of carbonylated or nitrated plasma proteins and fibrin polymerization or lysis in plasma isolated from invasive breast cancer patients. We also observed that the relationship between TAS and fibrin polymerization or lysis in plasma from invasive breast cancer exists.

CONCLUSION

Considering the data presented in this study, we suggest that the oxidative/nitrative stress in breast cancer patients may induce the changes of hemostasis in these patients.

Authors+Show Affiliations

Department of General Biochemistry, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22200561

Citation

Kedzierska, Magdalena, et al. "Relationship Between Thiol, Tyrosine Nitration and Carbonyl Formation as Biomarkers of Oxidative Stress and Changes of Hemostatic Function of Plasma From Breast Cancer Patients Before Surgery." Clinical Biochemistry, vol. 45, no. 3, 2012, pp. 231-6.
Kedzierska M, Olas B, Wachowicz B, et al. Relationship between thiol, tyrosine nitration and carbonyl formation as biomarkers of oxidative stress and changes of hemostatic function of plasma from breast cancer patients before surgery. Clin Biochem. 2012;45(3):231-6.
Kedzierska, M., Olas, B., Wachowicz, B., Jeziorski, A., & Piekarski, J. (2012). Relationship between thiol, tyrosine nitration and carbonyl formation as biomarkers of oxidative stress and changes of hemostatic function of plasma from breast cancer patients before surgery. Clinical Biochemistry, 45(3), pp. 231-6. doi:10.1016/j.clinbiochem.2011.12.002.
Kedzierska M, et al. Relationship Between Thiol, Tyrosine Nitration and Carbonyl Formation as Biomarkers of Oxidative Stress and Changes of Hemostatic Function of Plasma From Breast Cancer Patients Before Surgery. Clin Biochem. 2012;45(3):231-6. PubMed PMID: 22200561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between thiol, tyrosine nitration and carbonyl formation as biomarkers of oxidative stress and changes of hemostatic function of plasma from breast cancer patients before surgery. AU - Kedzierska,Magdalena, AU - Olas,Beata, AU - Wachowicz,Barbara, AU - Jeziorski,Arkadiusz, AU - Piekarski,Janusz, Y1 - 2011/12/19/ PY - 2011/09/28/received PY - 2011/12/01/revised PY - 2011/12/02/accepted PY - 2011/12/28/entrez PY - 2011/12/28/pubmed PY - 2012/5/25/medline SP - 231 EP - 6 JF - Clinical biochemistry JO - Clin. Biochem. VL - 45 IS - 3 N2 - OBJECTIVES: In breast cancer patients dysregulation of reactive oxygen species metabolism, as detected by various indicators in plasma or various blood cells, including red blood cells and blood platelets, is observed. DESIGN AND METHODS: The aim of our study was to explain the effect of oxidative/nitrative stress on hemostatic activity of plasma from breast cancer patients. Different methods were used to analyze oxidative/nitrative stress (carbonyl groups and 3-nitrotyrosine levels - ELISA and C-ELISA method, respectively and free thiol groups was estimated with 5,5'-dithio-bis(2-nitro-benzoic acid)). We also measured total antioxidant status (TAS) in plasma. RESULTS: Our results showed the correlation between the oxidative stress and changes of hemostasis in breast cancer patients; the correlation between the increased amount of carbonylated or nitrated plasma proteins and fibrin polymerization or lysis in plasma isolated from invasive breast cancer patients. We also observed that the relationship between TAS and fibrin polymerization or lysis in plasma from invasive breast cancer exists. CONCLUSION: Considering the data presented in this study, we suggest that the oxidative/nitrative stress in breast cancer patients may induce the changes of hemostasis in these patients. SN - 1873-2933 UR - https://www.unboundmedicine.com/medline/citation/22200561/Relationship_between_thiol_tyrosine_nitration_and_carbonyl_formation_as_biomarkers_of_oxidative_stress_and_changes_of_hemostatic_function_of_plasma_from_breast_cancer_patients_before_surgery_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-9120(11)02747-0 DB - PRIME DP - Unbound Medicine ER -