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A Leishmania infantum FML-ELISA for the detection of symptomatic and asymptomatic canine visceral leishmaniasis in an endemic area of Iran.
Iran J Immunol. 2011 Dec; 8(4):244-50.IJ

Abstract

BACKGROUND

Visceral leishmaniasis (VL) is caused by Leishmania infantum in Mediterranean basin and is an endemic disease in some parts of Iran. Canines are the main reservoirs of VL in most of the endemic areas. Different serological methods have been introduced for diagnosis of canine visceral leishmaniasis (CVL).

OBJECTIVE

In this survey a Fucose-Mannose Ligand (FML) ELISA, using native L. infantum antigen, was developed and its validity for detection of infected dogs in comparison with direct agglutination test (DAT) and PCR was evaluated.

METHODS

Blood samples of sixty ownership dogs (≤ 3 years old) were collected from Meshkin-shahr district in Ardabil province, North-west of Iran. Sera were separated for serological assays (DAT and FML-ELISA) and the buffy coats were collected for molecular evaluation.

RESULTS

Two out of the 60 (3.33%) samples were found to be positive (antibody titer of ≥ 1/320) in DAT while seven of the 60 (11.66%) samples were positive by FML-ELISA. Nine out of 60 (15%) buffy coat samples showed a band about 680 bp indicative of L. infantum in PCR. Three out of 60 dogs had Kala-azar symptoms and were positive by PCR and FML-ELISA, while two of these three dogs had antibody titers >1/320 in their serum samples. The sensitivity and specificity of FML-ELISA for the detection of CVL in both symptomatic and asymptomatic dogs were found to be 77.8% and 100%, respectively.

CONCLUSION

Considering the acceptable sensitivity and high specificity of FML-ELISA, use of this serological method can be recommended for epidemiological surveys of CVL.

Authors+Show Affiliations

Center for Basic Researches in Infectious Diseases, Shiraz University of Medical Sciences, Shiraz, Iran,e-mail: hatamghr@sums.ac.ir.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22201622

Citation

Mohammadi-Ghalehbin, Behnam, et al. "A Leishmania Infantum FML-ELISA for the Detection of Symptomatic and Asymptomatic Canine Visceral Leishmaniasis in an Endemic Area of Iran." Iranian Journal of Immunology : IJI, vol. 8, no. 4, 2011, pp. 244-50.
Mohammadi-Ghalehbin B, Hatam GR, Sarkari B, et al. A Leishmania infantum FML-ELISA for the detection of symptomatic and asymptomatic canine visceral leishmaniasis in an endemic area of Iran. Iran J Immunol. 2011;8(4):244-50.
Mohammadi-Ghalehbin, B., Hatam, G. R., Sarkari, B., Mohebali, M., Zare, Z., Jaberipour, M., & Bohlouli, S. (2011). A Leishmania infantum FML-ELISA for the detection of symptomatic and asymptomatic canine visceral leishmaniasis in an endemic area of Iran. Iranian Journal of Immunology : IJI, 8(4), 244-50. https://doi.org/IJIv8i4A7
Mohammadi-Ghalehbin B, et al. A Leishmania Infantum FML-ELISA for the Detection of Symptomatic and Asymptomatic Canine Visceral Leishmaniasis in an Endemic Area of Iran. Iran J Immunol. 2011;8(4):244-50. PubMed PMID: 22201622.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Leishmania infantum FML-ELISA for the detection of symptomatic and asymptomatic canine visceral leishmaniasis in an endemic area of Iran. AU - Mohammadi-Ghalehbin,Behnam, AU - Hatam,Gholam Reza, AU - Sarkari,Bahador, AU - Mohebali,Mehdi, AU - Zare,Zabih, AU - Jaberipour,Mansoureh, AU - Bohlouli,Shahab, PY - 2011/12/29/entrez PY - 2011/12/29/pubmed PY - 2014/7/22/medline SP - 244 EP - 50 JF - Iranian journal of immunology : IJI JO - Iran J Immunol VL - 8 IS - 4 N2 - BACKGROUND: Visceral leishmaniasis (VL) is caused by Leishmania infantum in Mediterranean basin and is an endemic disease in some parts of Iran. Canines are the main reservoirs of VL in most of the endemic areas. Different serological methods have been introduced for diagnosis of canine visceral leishmaniasis (CVL). OBJECTIVE: In this survey a Fucose-Mannose Ligand (FML) ELISA, using native L. infantum antigen, was developed and its validity for detection of infected dogs in comparison with direct agglutination test (DAT) and PCR was evaluated. METHODS: Blood samples of sixty ownership dogs (≤ 3 years old) were collected from Meshkin-shahr district in Ardabil province, North-west of Iran. Sera were separated for serological assays (DAT and FML-ELISA) and the buffy coats were collected for molecular evaluation. RESULTS: Two out of the 60 (3.33%) samples were found to be positive (antibody titer of ≥ 1/320) in DAT while seven of the 60 (11.66%) samples were positive by FML-ELISA. Nine out of 60 (15%) buffy coat samples showed a band about 680 bp indicative of L. infantum in PCR. Three out of 60 dogs had Kala-azar symptoms and were positive by PCR and FML-ELISA, while two of these three dogs had antibody titers >1/320 in their serum samples. The sensitivity and specificity of FML-ELISA for the detection of CVL in both symptomatic and asymptomatic dogs were found to be 77.8% and 100%, respectively. CONCLUSION: Considering the acceptable sensitivity and high specificity of FML-ELISA, use of this serological method can be recommended for epidemiological surveys of CVL. SN - 1735-367X UR - https://www.unboundmedicine.com/medline/citation/22201622/A_Leishmania_infantum_FML_ELISA_for_the_detection_of_symptomatic_and_asymptomatic_canine_visceral_leishmaniasis_in_an_endemic_area_of_Iran_ L2 - https://iji.sums.ac.ir/?sid=Entrez:PubMed&id=pmid:22201622&key=2011.8.4.244 DB - PRIME DP - Unbound Medicine ER -