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Function of the syndecan-4 cytoplasmic domain in oligomerization and association with α-actinin in turkey muscle satellite cells.
Mol Cell Biochem. 2012 Apr; 363(1-2):437-44.MC

Abstract

Syndecan-4 (S4) is a cell membrane heparan sulfate proteoglycan that plays a role in satellite cell mediated myogenesis. S4 modulates the proliferation of myogenic satellite cells, but the mechanism of how S4 functions during myogenesis is not well understood. In other cell systems, S4 has been shown to form oligomers in the cell membrane and interact through its cytoplasmic domain with the cytoskeletal protein α-actinin. This study addressed if S4 forms oligomers and interacts with α-actinin in muscle. The S4 cytoplasmic domain was found to interact with α-actinin in a phosphatidylinositol-4,5-bisphosphate dependent manner, but did not associate with vinculin. Through confocal microscopy, both S4 and syndecan-4 without the cytoplasmic domain were localized to the cell membrane. Although the cytoplasmic domain was necessary for the interaction with α-actinin, S4 oligomer formation occurred in the absence of the cytoplasmic domain. These data indicated that S4 function in skeletal muscle is mediated through the formation of oligomers and interaction with the cytoskeletal protein α-actinin.

Authors+Show Affiliations

Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, 213 Gerlaugh Hall, 1680 Madison Avenue, Wooster, OH 44691, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22203420

Citation

Shin, Jonghyun, et al. "Function of the Syndecan-4 Cytoplasmic Domain in Oligomerization and Association With Α-actinin in Turkey Muscle Satellite Cells." Molecular and Cellular Biochemistry, vol. 363, no. 1-2, 2012, pp. 437-44.
Shin J, Song Y, McFarland DC, et al. Function of the syndecan-4 cytoplasmic domain in oligomerization and association with α-actinin in turkey muscle satellite cells. Mol Cell Biochem. 2012;363(1-2):437-44.
Shin, J., Song, Y., McFarland, D. C., & Velleman, S. G. (2012). Function of the syndecan-4 cytoplasmic domain in oligomerization and association with α-actinin in turkey muscle satellite cells. Molecular and Cellular Biochemistry, 363(1-2), 437-44. https://doi.org/10.1007/s11010-011-1198-2
Shin J, et al. Function of the Syndecan-4 Cytoplasmic Domain in Oligomerization and Association With Α-actinin in Turkey Muscle Satellite Cells. Mol Cell Biochem. 2012;363(1-2):437-44. PubMed PMID: 22203420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Function of the syndecan-4 cytoplasmic domain in oligomerization and association with α-actinin in turkey muscle satellite cells. AU - Shin,Jonghyun, AU - Song,Yan, AU - McFarland,Douglas C, AU - Velleman,Sandra G, Y1 - 2011/12/28/ PY - 2011/10/26/received PY - 2011/12/13/accepted PY - 2011/12/29/entrez PY - 2011/12/29/pubmed PY - 2012/7/3/medline SP - 437 EP - 44 JF - Molecular and cellular biochemistry JO - Mol Cell Biochem VL - 363 IS - 1-2 N2 - Syndecan-4 (S4) is a cell membrane heparan sulfate proteoglycan that plays a role in satellite cell mediated myogenesis. S4 modulates the proliferation of myogenic satellite cells, but the mechanism of how S4 functions during myogenesis is not well understood. In other cell systems, S4 has been shown to form oligomers in the cell membrane and interact through its cytoplasmic domain with the cytoskeletal protein α-actinin. This study addressed if S4 forms oligomers and interacts with α-actinin in muscle. The S4 cytoplasmic domain was found to interact with α-actinin in a phosphatidylinositol-4,5-bisphosphate dependent manner, but did not associate with vinculin. Through confocal microscopy, both S4 and syndecan-4 without the cytoplasmic domain were localized to the cell membrane. Although the cytoplasmic domain was necessary for the interaction with α-actinin, S4 oligomer formation occurred in the absence of the cytoplasmic domain. These data indicated that S4 function in skeletal muscle is mediated through the formation of oligomers and interaction with the cytoskeletal protein α-actinin. SN - 1573-4919 UR - https://www.unboundmedicine.com/medline/citation/22203420/Function_of_the_syndecan_4_cytoplasmic_domain_in_oligomerization_and_association_with_α_actinin_in_turkey_muscle_satellite_cells_ L2 - https://doi.org/10.1007/s11010-011-1198-2 DB - PRIME DP - Unbound Medicine ER -