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Orf virus DNA vaccines expressing ORFV 011 and ORFV 059 chimeric protein enhances immunogenicity.
Virol J 2011; 8:562VJ

Abstract

BACKGROUND

ORFV attenuated live vaccines have been the main prophylactic measure against contagious ecthyma in sheep and goats in the last decades, which play an important role in preventing the outbreak of the disease. However, the available vaccines do not induce lasting immunity in sheep and goats. On the other hand, variation in the terminal genome of Orf virus vaccine strains during cell culture adaptation may affect the efficacy of a vaccine. Currently, there are no more effective antiviral treatments available for contagious ecthyma.

RESULTS

We constructed three eukaryotic expression vectors pcDNA3.1-ORFV011, pcDNA3.1-ORFV059 and pcDNA3.1-ORFV011/ORFV059 and tested their immunogenicity in mouse model. High level expression of the recombinant proteins ORFV011, ORFV059 and ORFV011/ORFV059 was confirmed by western blotting analysis and indirect fluorescence antibody (IFA) tests. The ORFV-specific antibody titers and serum IgG1/IgG2a titers, the proliferation of lymphocytes and ORFV-specific cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were examined to evaluate the immune responses of the vaccinated mice. We found that mice inoculated with pcDNA3.1-ORFV 011/ORFV059 had significantly stronger immunological responses than those inoculated with pcDNA3.1-ORFV011, pcDNA3.1-ORFV059, or pcDNA3.1-ORFV011 plus pcDNA3.1-ORFV059. Compared to other vaccine plasmids immunized groups, pcDNA3.1-ORFV011/ORFV059 immunized group enhances immunogenicity.

CONCLUSIONS

We concluded that DNA vaccine pcDNA3.1-ORFV011/ORFV059 expressing ORFV011 and ORFV059 chemeric-proteins can significantly improve the potency of DNA vaccination and could be served as more effective and safe approach for new vaccines against ORFV.

Authors+Show Affiliations

College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22204310

Citation

Zhao, Kui, et al. "Orf Virus DNA Vaccines Expressing ORFV 011 and ORFV 059 Chimeric Protein Enhances Immunogenicity." Virology Journal, vol. 8, 2011, p. 562.
Zhao K, He W, Gao W, et al. Orf virus DNA vaccines expressing ORFV 011 and ORFV 059 chimeric protein enhances immunogenicity. Virol J. 2011;8:562.
Zhao, K., He, W., Gao, W., Lu, H., Han, T., Li, J., ... Gao, F. (2011). Orf virus DNA vaccines expressing ORFV 011 and ORFV 059 chimeric protein enhances immunogenicity. Virology Journal, 8, p. 562. doi:10.1186/1743-422X-8-562.
Zhao K, et al. Orf Virus DNA Vaccines Expressing ORFV 011 and ORFV 059 Chimeric Protein Enhances Immunogenicity. Virol J. 2011 Dec 29;8:562. PubMed PMID: 22204310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Orf virus DNA vaccines expressing ORFV 011 and ORFV 059 chimeric protein enhances immunogenicity. AU - Zhao,Kui, AU - He,Wenqi, AU - Gao,Wei, AU - Lu,Huijun, AU - Han,Tiesuo, AU - Li,Jing, AU - Zhang,Ximu, AU - Zhang,Bingbing, AU - Wang,Gaili, AU - Su,Gaoli, AU - Zhao,Zhihui, AU - Song,Deguang, AU - Gao,Feng, Y1 - 2011/12/29/ PY - 2011/08/02/received PY - 2011/12/29/accepted PY - 2011/12/30/entrez PY - 2011/12/30/pubmed PY - 2012/12/10/medline SP - 562 EP - 562 JF - Virology journal JO - Virol. J. VL - 8 N2 - BACKGROUND: ORFV attenuated live vaccines have been the main prophylactic measure against contagious ecthyma in sheep and goats in the last decades, which play an important role in preventing the outbreak of the disease. However, the available vaccines do not induce lasting immunity in sheep and goats. On the other hand, variation in the terminal genome of Orf virus vaccine strains during cell culture adaptation may affect the efficacy of a vaccine. Currently, there are no more effective antiviral treatments available for contagious ecthyma. RESULTS: We constructed three eukaryotic expression vectors pcDNA3.1-ORFV011, pcDNA3.1-ORFV059 and pcDNA3.1-ORFV011/ORFV059 and tested their immunogenicity in mouse model. High level expression of the recombinant proteins ORFV011, ORFV059 and ORFV011/ORFV059 was confirmed by western blotting analysis and indirect fluorescence antibody (IFA) tests. The ORFV-specific antibody titers and serum IgG1/IgG2a titers, the proliferation of lymphocytes and ORFV-specific cytokines (IL-2, IL-4, IL-6, IFN-γ, and TNF-α) were examined to evaluate the immune responses of the vaccinated mice. We found that mice inoculated with pcDNA3.1-ORFV 011/ORFV059 had significantly stronger immunological responses than those inoculated with pcDNA3.1-ORFV011, pcDNA3.1-ORFV059, or pcDNA3.1-ORFV011 plus pcDNA3.1-ORFV059. Compared to other vaccine plasmids immunized groups, pcDNA3.1-ORFV011/ORFV059 immunized group enhances immunogenicity. CONCLUSIONS: We concluded that DNA vaccine pcDNA3.1-ORFV011/ORFV059 expressing ORFV011 and ORFV059 chemeric-proteins can significantly improve the potency of DNA vaccination and could be served as more effective and safe approach for new vaccines against ORFV. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/22204310/Orf_virus_DNA_vaccines_expressing_ORFV_011_and_ORFV_059_chimeric_protein_enhances_immunogenicity_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-8-562 DB - PRIME DP - Unbound Medicine ER -