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Activation of the Arabidopsis thaliana mitogen-activated protein kinase MPK11 by the flagellin-derived elicitor peptide, flg22.
Mol Plant Microbe Interact. 2012 Apr; 25(4):471-80.MP

Abstract

Mitogen-activated protein kinases (MAPK) mediate cellular signal transduction during stress responses, as well as diverse growth and developmental processes in eukaryotes. Pathogen infection or treatments with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPK: MPK3, MPK4, and MPK6. Several stresses, including flg22 treatment, are known to increase MPK11 expression but activation of MPK11 has not been shown. Here, we show that MPK11 activity can, indeed, be increased through flg22 elicitation. A small-scale microarray for profiling defense-related genes revealed that cinnamyl alcohol dehyrogenase 5 requires MPK11 for full flg22-induced expression. An mpk11 mutant showed increased flg22-mediated growth inhibition but no altered susceptibility to Pseudomonas syringae, Botrytis cinerea, or Alternaria brassicicola. In mpk3, mpk6, or mpk4 backgrounds, MPK11 is required for embryo or seed development or general viability. Although this developmental deficiency in double mutants and the lack of or only subtle mpk11 phenotypes suggest functional MAPK redundancies, comparison with the paralogous MPK4 reveals distinct functions. Taken together, future investigations of MAPK roles in stress signaling should include MPK11 as a fourth PAMP-activated MAPK.

Authors+Show Affiliations

Leibniz Institute of Plant Biochemistry, Stress and Developmental Biology, Weinberg 3, D-06120 Halle, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22204645

Citation

Bethke, Gerit, et al. "Activation of the Arabidopsis Thaliana Mitogen-activated Protein Kinase MPK11 By the Flagellin-derived Elicitor Peptide, Flg22." Molecular Plant-microbe Interactions : MPMI, vol. 25, no. 4, 2012, pp. 471-80.
Bethke G, Pecher P, Eschen-Lippold L, et al. Activation of the Arabidopsis thaliana mitogen-activated protein kinase MPK11 by the flagellin-derived elicitor peptide, flg22. Mol Plant Microbe Interact. 2012;25(4):471-80.
Bethke, G., Pecher, P., Eschen-Lippold, L., Tsuda, K., Katagiri, F., Glazebrook, J., Scheel, D., & Lee, J. (2012). Activation of the Arabidopsis thaliana mitogen-activated protein kinase MPK11 by the flagellin-derived elicitor peptide, flg22. Molecular Plant-microbe Interactions : MPMI, 25(4), 471-80. https://doi.org/10.1094/MPMI-11-11-0281
Bethke G, et al. Activation of the Arabidopsis Thaliana Mitogen-activated Protein Kinase MPK11 By the Flagellin-derived Elicitor Peptide, Flg22. Mol Plant Microbe Interact. 2012;25(4):471-80. PubMed PMID: 22204645.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of the Arabidopsis thaliana mitogen-activated protein kinase MPK11 by the flagellin-derived elicitor peptide, flg22. AU - Bethke,Gerit, AU - Pecher,Pascal, AU - Eschen-Lippold,Lennart, AU - Tsuda,Kenichi, AU - Katagiri,Fumiaki, AU - Glazebrook,Jane, AU - Scheel,Dierk, AU - Lee,Justin, PY - 2011/12/30/entrez PY - 2011/12/30/pubmed PY - 2012/5/18/medline SP - 471 EP - 80 JF - Molecular plant-microbe interactions : MPMI JO - Mol. Plant Microbe Interact. VL - 25 IS - 4 N2 - Mitogen-activated protein kinases (MAPK) mediate cellular signal transduction during stress responses, as well as diverse growth and developmental processes in eukaryotes. Pathogen infection or treatments with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPK: MPK3, MPK4, and MPK6. Several stresses, including flg22 treatment, are known to increase MPK11 expression but activation of MPK11 has not been shown. Here, we show that MPK11 activity can, indeed, be increased through flg22 elicitation. A small-scale microarray for profiling defense-related genes revealed that cinnamyl alcohol dehyrogenase 5 requires MPK11 for full flg22-induced expression. An mpk11 mutant showed increased flg22-mediated growth inhibition but no altered susceptibility to Pseudomonas syringae, Botrytis cinerea, or Alternaria brassicicola. In mpk3, mpk6, or mpk4 backgrounds, MPK11 is required for embryo or seed development or general viability. Although this developmental deficiency in double mutants and the lack of or only subtle mpk11 phenotypes suggest functional MAPK redundancies, comparison with the paralogous MPK4 reveals distinct functions. Taken together, future investigations of MAPK roles in stress signaling should include MPK11 as a fourth PAMP-activated MAPK. SN - 0894-0282 UR - https://www.unboundmedicine.com/medline/citation/22204645/Activation_of_the_Arabidopsis_thaliana_mitogen_activated_protein_kinase_MPK11_by_the_flagellin_derived_elicitor_peptide_flg22_ L2 - http://apsjournals.apsnet.org/doi/full/10.1094/MPMI-11-11-0281?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -