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Molecular profiles of IgE to Phleum pratense in children with grass pollen allergy: implications for specific immunotherapy.
J Allergy Clin Immunol. 2012 Mar; 129(3):834-839.e8.JA

Abstract

BACKGROUND

The so-called component-resolved immunotherapy of allergies proposes an immunization tailored to the molecular sensitization profiles of individual patients.

OBJECTIVES

We sought (1) to investigate the profiles of IgE sensitization to Phleum pratense in children with grass pollen allergy and (2) to define the compatibility of these profiles with a mixture of recombinant allergenic molecules of P pratense previously proposed for specific immunotherapy.

METHODS

We examined 200 children (age, 4-18 years; 126 boys) with allergic rhinitis, asthma, or both ascertained through validated questionnaires. Each child underwent skin prick testing (ALK-Abelló) and serum IgE assays (ImmunoCAP, Phadia) with 9 pollen extracts. Sera reacting against P pratense were tested for the individual molecules (rPhl p 1, rPhl p 2, rPhl p 4, nPhl p 4, rPhl p 5b, rPhl p 6, rPhl p 7, rPhl p 11, and Phl p 12). Through a combinatorial approach, the IgE individual sensitization profiles were matched against an experimental allergen-specific immunotherapy (SIT) preparation containing Phl p 1, Phl p 2, Phl p 5, and Phl p 6.

RESULTS

Among the 176 of 200 children with IgE sensitization to P pratense extract, 39 profiles of sensitization to the 8 allergenic molecules tested (cutoff, 0.35 kU/L) were identified. This high heterogeneity was reduced by considering only 6 or 4 P pratense molecules but not by increasing the cutoff levels of IgE positivity. The molecular profile of the experimental SIT preparation matched that of 7 (4%) of 176 patients only; the remaining 169 patients were classified in 4 mismatch categories: underpowered (29%), overpowered (32%), underpowered/overpowered (32%), and unrelated (3%).

CONCLUSIONS

IgE sensitization profiles to P pratense are highly heterogeneous. Molecularly designed SIT preparations tailored to patients' needs should consider this high heterogeneity and be driven by locally performed population studies.

Authors+Show Affiliations

Pediatric Allergology Unit, Sandro Pertini Hospital, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22206774

Citation

Tripodi, Salvatore, et al. "Molecular Profiles of IgE to Phleum Pratense in Children With Grass Pollen Allergy: Implications for Specific Immunotherapy." The Journal of Allergy and Clinical Immunology, vol. 129, no. 3, 2012, pp. 834-839.e8.
Tripodi S, Frediani T, Lucarelli S, et al. Molecular profiles of IgE to Phleum pratense in children with grass pollen allergy: implications for specific immunotherapy. J Allergy Clin Immunol. 2012;129(3):834-839.e8.
Tripodi, S., Frediani, T., Lucarelli, S., Macrì, F., Pingitore, G., Di Rienzo Businco, A., Dondi, A., Pansa, P., Ragusa, G., Asero, R., Faggian, D., Plebani, M., & Matricardi, P. M. (2012). Molecular profiles of IgE to Phleum pratense in children with grass pollen allergy: implications for specific immunotherapy. The Journal of Allergy and Clinical Immunology, 129(3), 834-e8. https://doi.org/10.1016/j.jaci.2011.10.045
Tripodi S, et al. Molecular Profiles of IgE to Phleum Pratense in Children With Grass Pollen Allergy: Implications for Specific Immunotherapy. J Allergy Clin Immunol. 2012;129(3):834-839.e8. PubMed PMID: 22206774.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular profiles of IgE to Phleum pratense in children with grass pollen allergy: implications for specific immunotherapy. AU - Tripodi,Salvatore, AU - Frediani,Tullio, AU - Lucarelli,Sandra, AU - Macrì,Francesco, AU - Pingitore,Giuseppe, AU - Di Rienzo Businco,Andrea, AU - Dondi,Arianna, AU - Pansa,Paola, AU - Ragusa,Giovanni, AU - Asero,Riccardo, AU - Faggian,Diego, AU - Plebani,Mario, AU - Matricardi,Paolo Maria, Y1 - 2011/12/28/ PY - 2011/07/29/received PY - 2011/09/26/revised PY - 2011/10/20/accepted PY - 2011/12/31/entrez PY - 2011/12/31/pubmed PY - 2012/7/7/medline SP - 834 EP - 839.e8 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 129 IS - 3 N2 - BACKGROUND: The so-called component-resolved immunotherapy of allergies proposes an immunization tailored to the molecular sensitization profiles of individual patients. OBJECTIVES: We sought (1) to investigate the profiles of IgE sensitization to Phleum pratense in children with grass pollen allergy and (2) to define the compatibility of these profiles with a mixture of recombinant allergenic molecules of P pratense previously proposed for specific immunotherapy. METHODS: We examined 200 children (age, 4-18 years; 126 boys) with allergic rhinitis, asthma, or both ascertained through validated questionnaires. Each child underwent skin prick testing (ALK-Abelló) and serum IgE assays (ImmunoCAP, Phadia) with 9 pollen extracts. Sera reacting against P pratense were tested for the individual molecules (rPhl p 1, rPhl p 2, rPhl p 4, nPhl p 4, rPhl p 5b, rPhl p 6, rPhl p 7, rPhl p 11, and Phl p 12). Through a combinatorial approach, the IgE individual sensitization profiles were matched against an experimental allergen-specific immunotherapy (SIT) preparation containing Phl p 1, Phl p 2, Phl p 5, and Phl p 6. RESULTS: Among the 176 of 200 children with IgE sensitization to P pratense extract, 39 profiles of sensitization to the 8 allergenic molecules tested (cutoff, 0.35 kU/L) were identified. This high heterogeneity was reduced by considering only 6 or 4 P pratense molecules but not by increasing the cutoff levels of IgE positivity. The molecular profile of the experimental SIT preparation matched that of 7 (4%) of 176 patients only; the remaining 169 patients were classified in 4 mismatch categories: underpowered (29%), overpowered (32%), underpowered/overpowered (32%), and unrelated (3%). CONCLUSIONS: IgE sensitization profiles to P pratense are highly heterogeneous. Molecularly designed SIT preparations tailored to patients' needs should consider this high heterogeneity and be driven by locally performed population studies. SN - 1097-6825 UR - https://www.unboundmedicine.com/medline/citation/22206774/Molecular_profiles_of_IgE_to_Phleum_pratense_in_children_with_grass_pollen_allergy:_implications_for_specific_immunotherapy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(11)01742-8 DB - PRIME DP - Unbound Medicine ER -