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Recent experiments towards a model for fluid secretion in Rhodnius Upper Malpighian Tubules (UMT).
J Insect Physiol. 2012 Apr; 58(4):543-50.JI

Abstract

Three different methods have been used to improve a model for fluid secretion in Upper Malpighian Tubules (UMT) of the blood sucking insect Rhodnius prolixus. (I) In the first, UMT double perfusions in 5th instar Rhodnius were used to measure their fluid secretion rate. They were stimulated to secrete with 5-HT. Double perfusions allowed access separately to the basolateral and the apical cell membranes with pharmacological agents known to block different ion transport functions, namely ATPases, cotransporters and/or countertransporters and ion and water channels: ouabain, bafilomycin A1, furosemide, bumetanide, SITS, acetazolamide, amiloride, DPC, BaCl(2), pCMBS and DTT. The basic assumption is that changes in water movement reflect changes in ion transport mechanisms. (II) Intracellular Na(+) concentrations were measured with a fluorometric method in dissected R. prolixus UMT, under several experimental conditions. (III) ATPase activities were measured in R. prolixus UMT. A tentative model for the function of the UMT cell is presented. We find that (a) at the basolateral cell membrane, fundamental is a Na(+)-K(+)-2Cl(-) cotransporter; of intermediate importance are the Na(+)-K(+)-ATPase and a ouabain-insensitive Na(+)-ATPase, ion channels and Rp-MIP water channels. (b) At the apical cell membrane, most important are a V-H(+)-ATPase; and a K(+) and/or Na(+)-H(+) exchanger.

Authors+Show Affiliations

Instituto Venezolano de Investigaciones Científicas, Universidad Central de Venezuela, Caracas, Venezuela. adrianadinorahgamez@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22206885

Citation

Gámez, Adriana D., et al. "Recent Experiments Towards a Model for Fluid Secretion in Rhodnius Upper Malpighian Tubules (UMT)." Journal of Insect Physiology, vol. 58, no. 4, 2012, pp. 543-50.
Gámez AD, Gutiérrez AM, García R, et al. Recent experiments towards a model for fluid secretion in Rhodnius Upper Malpighian Tubules (UMT). J Insect Physiol. 2012;58(4):543-50.
Gámez, A. D., Gutiérrez, A. M., García, R., & Whittembury, G. (2012). Recent experiments towards a model for fluid secretion in Rhodnius Upper Malpighian Tubules (UMT). Journal of Insect Physiology, 58(4), 543-50. https://doi.org/10.1016/j.jinsphys.2011.12.008
Gámez AD, et al. Recent Experiments Towards a Model for Fluid Secretion in Rhodnius Upper Malpighian Tubules (UMT). J Insect Physiol. 2012;58(4):543-50. PubMed PMID: 22206885.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recent experiments towards a model for fluid secretion in Rhodnius Upper Malpighian Tubules (UMT). AU - Gámez,Adriana D, AU - Gutiérrez,A M, AU - García,Rafael, AU - Whittembury,G, Y1 - 2011/12/21/ PY - 2011/09/20/received PY - 2011/12/08/revised PY - 2011/12/14/accepted PY - 2011/12/31/entrez PY - 2011/12/31/pubmed PY - 2012/7/11/medline SP - 543 EP - 50 JF - Journal of insect physiology JO - J Insect Physiol VL - 58 IS - 4 N2 - Three different methods have been used to improve a model for fluid secretion in Upper Malpighian Tubules (UMT) of the blood sucking insect Rhodnius prolixus. (I) In the first, UMT double perfusions in 5th instar Rhodnius were used to measure their fluid secretion rate. They were stimulated to secrete with 5-HT. Double perfusions allowed access separately to the basolateral and the apical cell membranes with pharmacological agents known to block different ion transport functions, namely ATPases, cotransporters and/or countertransporters and ion and water channels: ouabain, bafilomycin A1, furosemide, bumetanide, SITS, acetazolamide, amiloride, DPC, BaCl(2), pCMBS and DTT. The basic assumption is that changes in water movement reflect changes in ion transport mechanisms. (II) Intracellular Na(+) concentrations were measured with a fluorometric method in dissected R. prolixus UMT, under several experimental conditions. (III) ATPase activities were measured in R. prolixus UMT. A tentative model for the function of the UMT cell is presented. We find that (a) at the basolateral cell membrane, fundamental is a Na(+)-K(+)-2Cl(-) cotransporter; of intermediate importance are the Na(+)-K(+)-ATPase and a ouabain-insensitive Na(+)-ATPase, ion channels and Rp-MIP water channels. (b) At the apical cell membrane, most important are a V-H(+)-ATPase; and a K(+) and/or Na(+)-H(+) exchanger. SN - 1879-1611 UR - https://www.unboundmedicine.com/medline/citation/22206885/Recent_experiments_towards_a_model_for_fluid_secretion_in_Rhodnius_Upper_Malpighian_Tubules__UMT__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1910(11)00352-0 DB - PRIME DP - Unbound Medicine ER -