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Interactive effects of a protein kinase AII inhibitor and testosterone on spatial learning in the Morris water maze.
Behav Brain Res. 2012 Mar 17; 228(2):432-9.BB

Abstract

Neurohormones such as testosterone (TE) are important in modulation of learning and memory. In the present study, we investigated the interactive effects of pre-training bilateral intra-hippocampal infusions of testosterone and H-89, a selective PKAII inhibitor, on spatial acquisition in the Morris water maze (MWM). Different doses of TE (20, 40 and 80 μg/side) and H-89 (5 and 10 μM/side) were administered 30 min before start of the training each day. Control animals received bilateral intra-hippocampal infusions of DMSO as vehicle for TE and H-89. Animals were trained for 4 days and each day included one block of four trials. The results of this study showed that bilateral infusion of TE (40 and 80 μg/side) or H-89 (10 μM/side) impaired spatial learning as indicated by significant increases in escape latency and traveled distance compared to the control group. Although pre-training bilateral infusions of a low concentration of either TE (20 μg/side) or H-89 (5 μM/side) into the CA1 region of the hippocampus did not affect learning capabilities, but the combination of the low doses of the drugs led to significant deficits in spatial acquisition. Overall, our data suggest that spatial acquisition was affected by PKAII inhibition or TE administration. Moreover, when co-administered, these drugs had a negative synergistic impact on acquisition.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22209852

Citation

Khorshidahmad, Tina, et al. "Interactive Effects of a Protein Kinase AII Inhibitor and Testosterone On Spatial Learning in the Morris Water Maze." Behavioural Brain Research, vol. 228, no. 2, 2012, pp. 432-9.
Khorshidahmad T, Tabrizian K, Vakilzadeh G, et al. Interactive effects of a protein kinase AII inhibitor and testosterone on spatial learning in the Morris water maze. Behav Brain Res. 2012;228(2):432-9.
Khorshidahmad, T., Tabrizian, K., Vakilzadeh, G., Nikbin, P., Moradi, S., Hosseini-Sharifabad, A., Roghani, A., Naghdi, N., & Sharifzadeh, M. (2012). Interactive effects of a protein kinase AII inhibitor and testosterone on spatial learning in the Morris water maze. Behavioural Brain Research, 228(2), 432-9. https://doi.org/10.1016/j.bbr.2011.12.028
Khorshidahmad T, et al. Interactive Effects of a Protein Kinase AII Inhibitor and Testosterone On Spatial Learning in the Morris Water Maze. Behav Brain Res. 2012 Mar 17;228(2):432-9. PubMed PMID: 22209852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interactive effects of a protein kinase AII inhibitor and testosterone on spatial learning in the Morris water maze. AU - Khorshidahmad,Tina, AU - Tabrizian,Kaveh, AU - Vakilzadeh,Gelareh, AU - Nikbin,Parmida, AU - Moradi,Shahla, AU - Hosseini-Sharifabad,Ali, AU - Roghani,Ali, AU - Naghdi,Nasser, AU - Sharifzadeh,Mohammad, Y1 - 2011/12/24/ PY - 2011/09/27/received PY - 2011/12/12/revised PY - 2011/12/16/accepted PY - 2012/1/3/entrez PY - 2012/1/3/pubmed PY - 2012/5/24/medline SP - 432 EP - 9 JF - Behavioural brain research JO - Behav Brain Res VL - 228 IS - 2 N2 - Neurohormones such as testosterone (TE) are important in modulation of learning and memory. In the present study, we investigated the interactive effects of pre-training bilateral intra-hippocampal infusions of testosterone and H-89, a selective PKAII inhibitor, on spatial acquisition in the Morris water maze (MWM). Different doses of TE (20, 40 and 80 μg/side) and H-89 (5 and 10 μM/side) were administered 30 min before start of the training each day. Control animals received bilateral intra-hippocampal infusions of DMSO as vehicle for TE and H-89. Animals were trained for 4 days and each day included one block of four trials. The results of this study showed that bilateral infusion of TE (40 and 80 μg/side) or H-89 (10 μM/side) impaired spatial learning as indicated by significant increases in escape latency and traveled distance compared to the control group. Although pre-training bilateral infusions of a low concentration of either TE (20 μg/side) or H-89 (5 μM/side) into the CA1 region of the hippocampus did not affect learning capabilities, but the combination of the low doses of the drugs led to significant deficits in spatial acquisition. Overall, our data suggest that spatial acquisition was affected by PKAII inhibition or TE administration. Moreover, when co-administered, these drugs had a negative synergistic impact on acquisition. SN - 1872-7549 UR - https://www.unboundmedicine.com/medline/citation/22209852/Interactive_effects_of_a_protein_kinase_AII_inhibitor_and_testosterone_on_spatial_learning_in_the_Morris_water_maze_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-4328(11)00902-8 DB - PRIME DP - Unbound Medicine ER -