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The immunoregulatory role of CD4⁺ FoxP3⁺ CD25⁻ regulatory T cells in lungs of mice infected with Bordetella pertussis.
FEMS Immunol Med Microbiol 2012; 64(3):413-24FI

Abstract

The identification of regulatory T (Treg) cells was originally based on CD25 expression; however, CD25 is also expressed by activated effector T cells. FoxP3 is a more definitive marker of Treg cells, and CD4(+) FoxP3(+) CD25(+) T cells are considered the dominant natural Treg (nTreg) population. It has been suggested that certain CD4(+) FoxP3(+) Treg cells do not express CD25. In this study, we used a murine model of respiratory infection with Bordetella pertussis to examine the role of Treg cells in protective immunity in the lung. We first demonstrated that CD4(+) FoxP3(+) CD25(-) cells are the dominant Treg population in the lung, gut and liver. Pre-activated lung CD4(+) FoxP3(+) CD25(-) cells suppressed CD4(+) effector T cells in vitro, which was partly mediated by IL-10 and not dependent on cell contact. Furthermore, CD4(+) FoxP3(+) CD25(-) IL-10(+) T cells were found in the lungs of mice at the peak of infection with B. pertussis. The rate of bacterial clearance was not affected by depletion of CD25(+) cells or in IL-10-deficient (IL-10(-/-)) mice, but was compromised in CD25-depleted IL-10(-/-) mice. Our findings suggest that IL-10-producing CD4(+) FoxP3(+) CD25(-) T cells represent an important regulatory cell in the lung.

Authors+Show Affiliations

Immune Regulation Research Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22211712

Citation

Coleman, Michelle M., et al. "The Immunoregulatory Role of CD4⁺ FoxP3⁺ CD25⁻ Regulatory T Cells in Lungs of Mice Infected With Bordetella Pertussis." FEMS Immunology and Medical Microbiology, vol. 64, no. 3, 2012, pp. 413-24.
Coleman MM, Finlay CM, Moran B, et al. The immunoregulatory role of CD4⁺ FoxP3⁺ CD25⁻ regulatory T cells in lungs of mice infected with Bordetella pertussis. FEMS Immunol Med Microbiol. 2012;64(3):413-24.
Coleman, M. M., Finlay, C. M., Moran, B., Keane, J., Dunne, P. J., & Mills, K. H. (2012). The immunoregulatory role of CD4⁺ FoxP3⁺ CD25⁻ regulatory T cells in lungs of mice infected with Bordetella pertussis. FEMS Immunology and Medical Microbiology, 64(3), pp. 413-24. doi:10.1111/j.1574-695X.2011.00927.x.
Coleman MM, et al. The Immunoregulatory Role of CD4⁺ FoxP3⁺ CD25⁻ Regulatory T Cells in Lungs of Mice Infected With Bordetella Pertussis. FEMS Immunol Med Microbiol. 2012;64(3):413-24. PubMed PMID: 22211712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The immunoregulatory role of CD4⁺ FoxP3⁺ CD25⁻ regulatory T cells in lungs of mice infected with Bordetella pertussis. AU - Coleman,Michelle M, AU - Finlay,Conor M, AU - Moran,Barry, AU - Keane,Joseph, AU - Dunne,Pádraic J, AU - Mills,Kingston H G, Y1 - 2012/01/18/ PY - 2011/08/12/received PY - 2011/12/09/revised PY - 2011/12/21/accepted PY - 2012/1/4/entrez PY - 2012/1/4/pubmed PY - 2012/9/12/medline SP - 413 EP - 24 JF - FEMS immunology and medical microbiology JO - FEMS Immunol. Med. Microbiol. VL - 64 IS - 3 N2 - The identification of regulatory T (Treg) cells was originally based on CD25 expression; however, CD25 is also expressed by activated effector T cells. FoxP3 is a more definitive marker of Treg cells, and CD4(+) FoxP3(+) CD25(+) T cells are considered the dominant natural Treg (nTreg) population. It has been suggested that certain CD4(+) FoxP3(+) Treg cells do not express CD25. In this study, we used a murine model of respiratory infection with Bordetella pertussis to examine the role of Treg cells in protective immunity in the lung. We first demonstrated that CD4(+) FoxP3(+) CD25(-) cells are the dominant Treg population in the lung, gut and liver. Pre-activated lung CD4(+) FoxP3(+) CD25(-) cells suppressed CD4(+) effector T cells in vitro, which was partly mediated by IL-10 and not dependent on cell contact. Furthermore, CD4(+) FoxP3(+) CD25(-) IL-10(+) T cells were found in the lungs of mice at the peak of infection with B. pertussis. The rate of bacterial clearance was not affected by depletion of CD25(+) cells or in IL-10-deficient (IL-10(-/-)) mice, but was compromised in CD25-depleted IL-10(-/-) mice. Our findings suggest that IL-10-producing CD4(+) FoxP3(+) CD25(-) T cells represent an important regulatory cell in the lung. SN - 1574-695X UR - https://www.unboundmedicine.com/medline/citation/22211712/The_immunoregulatory_role_of_CD4⁺_FoxP3⁺_CD25⁻_regulatory_T_cells_in_lungs_of_mice_infected_with_Bordetella_pertussis_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0928-8244&date=2012&volume=64&issue=3&spage=413 DB - PRIME DP - Unbound Medicine ER -