Prime

Type your tag names separated by a space and hit enter

Melatonin attenuates doxorubicin-induced testicular toxicity in rats.

Abstract

This study investigated the protective effects of melatonin (MLT) against doxorubicin (DXR)-induced testicular toxicity and oxidative stress in rats. DXR was given as a single intraperitoneal dose of 10 mg kg(-1) body weight to male rats at 1 h after MLT treatment on day 6 of the study. MLT at 15 mg kg(-1) body weight was administered daily by gavage for 5 days before DXR treatment followed by an additional dose for 5 days. Sperm analysis, histopathological examination and biochemical methods were used for this investigation. DXR caused a decrease in the weight of seminal vesicles, epididymal sperm count and motility and an increase in the incidence of histopathological changes of the testis. In addition, an increased malondialdehyde (MDA) concentration and decreased glutathione content, glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase activities were observed. On the contrary, MLT treatment significantly ameliorated DXR-induced testicular toxicity in rats. Moreover, MDA concentration and GR, GST and SOD activities were not affected when MLT was administered in conjunction with DXR. These results indicate that MLT had a protective effect against DXR-induced testicular toxicity and that the protective effects of MLT may be due to both the inhibition of lipid peroxidation and increased antioxidant activity.

Links

  • Publisher Full Text
  • Authors

    , , , , , , , , ,

    Source

    Andrologia 44 Suppl 1: 2012 May pg 796-803

    MeSH

    Animals
    Antibiotics, Antineoplastic
    Catalase
    Doxorubicin
    Glutathione
    Glutathione Reductase
    Glutathione Transferase
    Male
    Malondialdehyde
    Melatonin
    Rats
    Rats, Sprague-Dawley
    Superoxide Dismutase
    Testis

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22212014

    Citation

    TY - JOUR T1 - Melatonin attenuates doxorubicin-induced testicular toxicity in rats. AU - Lee,K-M, AU - Lee,I-C, AU - Kim,S-H, AU - Moon,C, AU - Park,S-H, AU - Shin,D-H, AU - Kim,S-H, AU - Park,S-C, AU - Kim,H-C, AU - Kim,J-C, Y1 - 2011/12/26/ PY - 2011/9/20/received PY - 2011/10/6/revised PY - 2011/11/1/accepted PY - 2011/12/26/aheadofprint PY - 2012/1/4/entrez PY - 2012/1/4/pubmed PY - 2012/9/28/medline SP - 796 EP - 803 JF - Andrologia JO - Andrologia VL - 44 Suppl 1 N2 - This study investigated the protective effects of melatonin (MLT) against doxorubicin (DXR)-induced testicular toxicity and oxidative stress in rats. DXR was given as a single intraperitoneal dose of 10 mg kg(-1) body weight to male rats at 1 h after MLT treatment on day 6 of the study. MLT at 15 mg kg(-1) body weight was administered daily by gavage for 5 days before DXR treatment followed by an additional dose for 5 days. Sperm analysis, histopathological examination and biochemical methods were used for this investigation. DXR caused a decrease in the weight of seminal vesicles, epididymal sperm count and motility and an increase in the incidence of histopathological changes of the testis. In addition, an increased malondialdehyde (MDA) concentration and decreased glutathione content, glutathione reductase (GR), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase activities were observed. On the contrary, MLT treatment significantly ameliorated DXR-induced testicular toxicity in rats. Moreover, MDA concentration and GR, GST and SOD activities were not affected when MLT was administered in conjunction with DXR. These results indicate that MLT had a protective effect against DXR-induced testicular toxicity and that the protective effects of MLT may be due to both the inhibition of lipid peroxidation and increased antioxidant activity. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/22212014/Melatonin_attenuates_doxorubicin_induced_testicular_toxicity_in_rats_ L2 - http://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0303-4569&date=2012&volume=44&issue=&spage=796 ER -