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Bioactive electroconductive hydrogels: the effects of electropolymerization charge density on the storage stability of an enzyme-based biosensor.
Appl Biochem Biotechnol. 2012 Feb; 166(4):878-88.AB

Abstract

Electrode-supported hydrogels were conferred with the biospecificity of enzymes during the process of electropolymerization to give rise to a class of bioactive, stimuli-responsive co-joined interpenetrating networks of inherently conductive polymers and highly hydrated hydrogels. Glucose responsive biotransducers were prepared by potentiostatic electropolymerization [750 mV vs. Ag/AgCl (3 M KCl)] of pyrrole at Poly(hydoxyethyl methacrylate)-based hydrogel-coated Pt micro-electrodes (Φ = 100 μm) from aqueous solutions of pyrrole and glucose oxidase (GOx; 0.4 M pyrrole, 1.0 mg/ml GOx) to 1.0 and 10.0 mC/cm². Polypyrrole was them over-oxidized by cyclic voltammetry (0-1.2 V vs. Ag/AgCl, 40 cycles in PBKCl, pH = 7.0). Biotransducers were stored at 4 °C in PBKCl for up to 18 days. Amperometric dose-response at 0.4 V vs. Ag/AgCl followed by Lineweaver-Burk analysis produced enzyme kinetic parameters as a function of electropolymerization charge density and storage time. Apparent Michaelis constant (K (Mapp)) increased from 18.6-152.0 mM (1.0 mC/cm²) and from 2.7-6.1 mM (10.0 mC/cm²). Biotransducer sensitivity increased to 21.2 nA/mM after 18 days and to 12.8 pA/mM after 10 days for the 1.0 and 10.0 mC/cm² membranes, respectively. Maximum current, I (max), also increased over time to 2.7 nA (1.0 mC/cm²) and to 170 pA (10.0 mC/cm²). Electropolymerization of polypyrrole is shown to be an effective means for imparting bioactivity to a hydrogel-coated microelectrode. GOx was shown to be stabilized and to increase activity over time within the electroconductive hydrogel.

Authors+Show Affiliations

Department of Bioengineering, Clemson University, 132 Earle Hall, Clemson, SC 29634, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22212391

Citation

Kotanen, Christian N., et al. "Bioactive Electroconductive Hydrogels: the Effects of Electropolymerization Charge Density On the Storage Stability of an Enzyme-based Biosensor." Applied Biochemistry and Biotechnology, vol. 166, no. 4, 2012, pp. 878-88.
Kotanen CN, Tlili C, Guiseppi-Elie A. Bioactive electroconductive hydrogels: the effects of electropolymerization charge density on the storage stability of an enzyme-based biosensor. Appl Biochem Biotechnol. 2012;166(4):878-88.
Kotanen, C. N., Tlili, C., & Guiseppi-Elie, A. (2012). Bioactive electroconductive hydrogels: the effects of electropolymerization charge density on the storage stability of an enzyme-based biosensor. Applied Biochemistry and Biotechnology, 166(4), 878-88. https://doi.org/10.1007/s12010-011-9477-7
Kotanen CN, Tlili C, Guiseppi-Elie A. Bioactive Electroconductive Hydrogels: the Effects of Electropolymerization Charge Density On the Storage Stability of an Enzyme-based Biosensor. Appl Biochem Biotechnol. 2012;166(4):878-88. PubMed PMID: 22212391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactive electroconductive hydrogels: the effects of electropolymerization charge density on the storage stability of an enzyme-based biosensor. AU - Kotanen,Christian N, AU - Tlili,Chaker, AU - Guiseppi-Elie,Anthony, Y1 - 2012/01/03/ PY - 2011/09/11/received PY - 2011/11/29/accepted PY - 2012/1/4/entrez PY - 2012/1/4/pubmed PY - 2012/5/26/medline SP - 878 EP - 88 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 166 IS - 4 N2 - Electrode-supported hydrogels were conferred with the biospecificity of enzymes during the process of electropolymerization to give rise to a class of bioactive, stimuli-responsive co-joined interpenetrating networks of inherently conductive polymers and highly hydrated hydrogels. Glucose responsive biotransducers were prepared by potentiostatic electropolymerization [750 mV vs. Ag/AgCl (3 M KCl)] of pyrrole at Poly(hydoxyethyl methacrylate)-based hydrogel-coated Pt micro-electrodes (Φ = 100 μm) from aqueous solutions of pyrrole and glucose oxidase (GOx; 0.4 M pyrrole, 1.0 mg/ml GOx) to 1.0 and 10.0 mC/cm². Polypyrrole was them over-oxidized by cyclic voltammetry (0-1.2 V vs. Ag/AgCl, 40 cycles in PBKCl, pH = 7.0). Biotransducers were stored at 4 °C in PBKCl for up to 18 days. Amperometric dose-response at 0.4 V vs. Ag/AgCl followed by Lineweaver-Burk analysis produced enzyme kinetic parameters as a function of electropolymerization charge density and storage time. Apparent Michaelis constant (K (Mapp)) increased from 18.6-152.0 mM (1.0 mC/cm²) and from 2.7-6.1 mM (10.0 mC/cm²). Biotransducer sensitivity increased to 21.2 nA/mM after 18 days and to 12.8 pA/mM after 10 days for the 1.0 and 10.0 mC/cm² membranes, respectively. Maximum current, I (max), also increased over time to 2.7 nA (1.0 mC/cm²) and to 170 pA (10.0 mC/cm²). Electropolymerization of polypyrrole is shown to be an effective means for imparting bioactivity to a hydrogel-coated microelectrode. GOx was shown to be stabilized and to increase activity over time within the electroconductive hydrogel. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/22212391/Bioactive_electroconductive_hydrogels:_the_effects_of_electropolymerization_charge_density_on_the_storage_stability_of_an_enzyme_based_biosensor_ L2 - https://dx.doi.org/10.1007/s12010-011-9477-7 DB - PRIME DP - Unbound Medicine ER -