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Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast.
Genes Cells. 2012 Jan; 17(1):39-52.GC

Abstract

ecl1+, ecl2+ and ecl3+ genes encode highly homologous small proteins, and their over-expressions confer both H2O2 stress resistance and chronological lifespan extension on Schizosaccharomyces pombe. However, the mechanisms of how these Ecl1 family proteins function have not been elucidated. In this study, we conducted microarray analysis and identified that the expression of genes involved in sexual development and stress responses was affected by the over-expression of Ecl1 family proteins. In agreement with the mRNA expression profile, the cells over-expressing Ecl1 family proteins showed high mating efficiency and resistant phenotype to H2O2. We showed that the H2O2-resistant phenotype depends on catalase Ctt1, and over-expression of ctt1+ does not affect chronological lifespan. Furthermore, we showed that six genes, ste11+, spk1+, hsr1+, rsv2+, hsp9+ and lsd90+, whose expressions are increased in cells over-expressing Ecl1 family proteins are involved in chronological lifespan in fission yeast. Among these genes, the induction of ste11+ and hsr1+ was dependent on a transcription factor Prr1, and we showed that the extensions of chronological lifespan by Ecl1 family proteins are remarkably diminished in prr1 deletion mutant. From these results, we propose that Ecl1-family proteins conduct H2O2 stress resistance and chronological lifespan extension in ctt1+- and prr1+-dependent manner, respectively.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22212525

Citation

Ohtsuka, Hokuto, et al. "Chronological Lifespan Extension By Ecl1 Family Proteins Depends On Prr1 Response Regulator in Fission Yeast." Genes to Cells : Devoted to Molecular & Cellular Mechanisms, vol. 17, no. 1, 2012, pp. 39-52.
Ohtsuka H, Azuma K, Kubota S, et al. Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast. Genes Cells. 2012;17(1):39-52.
Ohtsuka, H., Azuma, K., Kubota, S., Murakami, H., Giga-Hama, Y., Tohda, H., & Aiba, H. (2012). Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast. Genes to Cells : Devoted to Molecular & Cellular Mechanisms, 17(1), 39-52. https://doi.org/10.1111/j.1365-2443.2011.01571.x
Ohtsuka H, et al. Chronological Lifespan Extension By Ecl1 Family Proteins Depends On Prr1 Response Regulator in Fission Yeast. Genes Cells. 2012;17(1):39-52. PubMed PMID: 22212525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast. AU - Ohtsuka,Hokuto, AU - Azuma,Kenko, AU - Kubota,Sachiko, AU - Murakami,Hiroshi, AU - Giga-Hama,Yuko, AU - Tohda,Hideki, AU - Aiba,Hirofumi, PY - 2012/1/4/entrez PY - 2012/1/4/pubmed PY - 2013/2/27/medline SP - 39 EP - 52 JF - Genes to cells : devoted to molecular & cellular mechanisms JO - Genes Cells VL - 17 IS - 1 N2 - ecl1+, ecl2+ and ecl3+ genes encode highly homologous small proteins, and their over-expressions confer both H2O2 stress resistance and chronological lifespan extension on Schizosaccharomyces pombe. However, the mechanisms of how these Ecl1 family proteins function have not been elucidated. In this study, we conducted microarray analysis and identified that the expression of genes involved in sexual development and stress responses was affected by the over-expression of Ecl1 family proteins. In agreement with the mRNA expression profile, the cells over-expressing Ecl1 family proteins showed high mating efficiency and resistant phenotype to H2O2. We showed that the H2O2-resistant phenotype depends on catalase Ctt1, and over-expression of ctt1+ does not affect chronological lifespan. Furthermore, we showed that six genes, ste11+, spk1+, hsr1+, rsv2+, hsp9+ and lsd90+, whose expressions are increased in cells over-expressing Ecl1 family proteins are involved in chronological lifespan in fission yeast. Among these genes, the induction of ste11+ and hsr1+ was dependent on a transcription factor Prr1, and we showed that the extensions of chronological lifespan by Ecl1 family proteins are remarkably diminished in prr1 deletion mutant. From these results, we propose that Ecl1-family proteins conduct H2O2 stress resistance and chronological lifespan extension in ctt1+- and prr1+-dependent manner, respectively. SN - 1365-2443 UR - https://www.unboundmedicine.com/medline/citation/22212525/Chronological_lifespan_extension_by_Ecl1_family_proteins_depends_on_Prr1_response_regulator_in_fission_yeast_ L2 - https://doi.org/10.1111/j.1365-2443.2011.01571.x DB - PRIME DP - Unbound Medicine ER -