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Amyloid-β oligomers in cerebrospinal fluid are associated with cognitive decline in patients with Alzheimer's disease.
J Alzheimers Dis 2012; 29(1):171-6JA

Abstract

Oligomers of the amyloid-β peptide (Aβ) are thought to be the most toxic form of Aβ and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n = 14), non-demented controls (n = 12), and other neurological disorders (dementia with Lewy bodies, n = 2; vascular dementia, n = 1; primary progressive aphasia, n = 1) were analyzed for the presence of Aβ-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-β (Aβ)42 were determined using ELISA. CSF Aβ-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio Aβ-oligomers/Aβ42 was significantly elevated in AD subjects compared to non-AD subjects (p = 0.001). Most important, there was a negative correlation between the amount of Aβ-oligomers and the Mini-Mental Status Exam score (r = -0.65; p = 0.013) in AD patients. The detection of Aβ-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity.

Authors+Show Affiliations

Department of Cardiothoracic Surgery of the Martin-Luther-University Halle-Wittenberg, Halle, Germany. alexander.navarrete@medizin.unihalle.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22214781

Citation

Santos, Alexander Navarrete, et al. "Amyloid-β Oligomers in Cerebrospinal Fluid Are Associated With Cognitive Decline in Patients With Alzheimer's Disease." Journal of Alzheimer's Disease : JAD, vol. 29, no. 1, 2012, pp. 171-6.
Santos AN, Ewers M, Minthon L, et al. Amyloid-β oligomers in cerebrospinal fluid are associated with cognitive decline in patients with Alzheimer's disease. J Alzheimers Dis. 2012;29(1):171-6.
Santos, A. N., Ewers, M., Minthon, L., Simm, A., Silber, R. E., Blennow, K., ... Hampel, H. (2012). Amyloid-β oligomers in cerebrospinal fluid are associated with cognitive decline in patients with Alzheimer's disease. Journal of Alzheimer's Disease : JAD, 29(1), pp. 171-6. doi:10.3233/JAD-2012-111361.
Santos AN, et al. Amyloid-β Oligomers in Cerebrospinal Fluid Are Associated With Cognitive Decline in Patients With Alzheimer's Disease. J Alzheimers Dis. 2012;29(1):171-6. PubMed PMID: 22214781.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amyloid-β oligomers in cerebrospinal fluid are associated with cognitive decline in patients with Alzheimer's disease. AU - Santos,Alexander Navarrete, AU - Ewers,Michael, AU - Minthon,Lennart, AU - Simm,Andreas, AU - Silber,Rolf-Edgar, AU - Blennow,Kaj, AU - Prvulovic,David, AU - Hansson,Oskar, AU - Hampel,Harald, PY - 2012/1/5/entrez PY - 2012/1/5/pubmed PY - 2012/9/22/medline SP - 171 EP - 6 JF - Journal of Alzheimer's disease : JAD JO - J. Alzheimers Dis. VL - 29 IS - 1 N2 - Oligomers of the amyloid-β peptide (Aβ) are thought to be the most toxic form of Aβ and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n = 14), non-demented controls (n = 12), and other neurological disorders (dementia with Lewy bodies, n = 2; vascular dementia, n = 1; primary progressive aphasia, n = 1) were analyzed for the presence of Aβ-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-β (Aβ)42 were determined using ELISA. CSF Aβ-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio Aβ-oligomers/Aβ42 was significantly elevated in AD subjects compared to non-AD subjects (p = 0.001). Most important, there was a negative correlation between the amount of Aβ-oligomers and the Mini-Mental Status Exam score (r = -0.65; p = 0.013) in AD patients. The detection of Aβ-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity. SN - 1875-8908 UR - https://www.unboundmedicine.com/medline/citation/22214781/Amyloid_β_oligomers_in_cerebrospinal_fluid_are_associated_with_cognitive_decline_in_patients_with_Alzheimer's_disease_ L2 - https://content.iospress.com/openurl?genre=article&id=doi:10.3233/JAD-2012-111361 DB - PRIME DP - Unbound Medicine ER -