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Amyloid-β oligomers in cerebrospinal fluid are associated with cognitive decline in patients with Alzheimer's disease.

Abstract

Oligomers of the amyloid-β peptide (Aβ) are thought to be the most toxic form of Aβ and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n = 14), non-demented controls (n = 12), and other neurological disorders (dementia with Lewy bodies, n = 2; vascular dementia, n = 1; primary progressive aphasia, n = 1) were analyzed for the presence of Aβ-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-β (Aβ)42 were determined using ELISA. CSF Aβ-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio Aβ-oligomers/Aβ42 was significantly elevated in AD subjects compared to non-AD subjects (p = 0.001). Most important, there was a negative correlation between the amount of Aβ-oligomers and the Mini-Mental Status Exam score (r = -0.65; p = 0.013) in AD patients. The detection of Aβ-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity.

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  • Authors+Show Affiliations

    ,

    Department of Cardiothoracic Surgery of the Martin-Luther-University Halle-Wittenberg, Halle, Germany. alexander.navarrete@medizin.unihalle.de

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    Source

    MeSH

    Aged
    Aged, 80 and over
    Alzheimer Disease
    Amyloid beta-Peptides
    Biomarkers
    Case-Control Studies
    Cognition Disorders
    Female
    Humans
    Male
    Middle Aged
    Peptide Fragments

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22214781

    Citation

    Santos, Alexander Navarrete, et al. "Amyloid-β Oligomers in Cerebrospinal Fluid Are Associated With Cognitive Decline in Patients With Alzheimer's Disease." Journal of Alzheimer's Disease : JAD, vol. 29, no. 1, 2012, pp. 171-6.
    Santos AN, Ewers M, Minthon L, et al. Amyloid-β oligomers in cerebrospinal fluid are associated with cognitive decline in patients with Alzheimer's disease. J Alzheimers Dis. 2012;29(1):171-6.
    Santos, A. N., Ewers, M., Minthon, L., Simm, A., Silber, R. E., Blennow, K., ... Hampel, H. (2012). Amyloid-β oligomers in cerebrospinal fluid are associated with cognitive decline in patients with Alzheimer's disease. Journal of Alzheimer's Disease : JAD, 29(1), pp. 171-6. doi:10.3233/JAD-2012-111361.
    Santos AN, et al. Amyloid-β Oligomers in Cerebrospinal Fluid Are Associated With Cognitive Decline in Patients With Alzheimer's Disease. J Alzheimers Dis. 2012;29(1):171-6. PubMed PMID: 22214781.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Amyloid-β oligomers in cerebrospinal fluid are associated with cognitive decline in patients with Alzheimer's disease. AU - Santos,Alexander Navarrete, AU - Ewers,Michael, AU - Minthon,Lennart, AU - Simm,Andreas, AU - Silber,Rolf-Edgar, AU - Blennow,Kaj, AU - Prvulovic,David, AU - Hansson,Oskar, AU - Hampel,Harald, PY - 2012/1/5/entrez PY - 2012/1/5/pubmed PY - 2012/9/22/medline SP - 171 EP - 6 JF - Journal of Alzheimer's disease : JAD JO - J. Alzheimers Dis. VL - 29 IS - 1 N2 - Oligomers of the amyloid-β peptide (Aβ) are thought to be the most toxic form of Aβ and are linked to the development of Alzheimer's disease (AD). Here, we used a flow cytometric approach for the detection and assessment of oligomers in cerebrospinal fluid (CSF) from AD patients and other neurological disorders. 30 CSF samples from patients suffering from AD (n = 14), non-demented controls (n = 12), and other neurological disorders (dementia with Lewy bodies, n = 2; vascular dementia, n = 1; primary progressive aphasia, n = 1) were analyzed for the presence of Aβ-oligomers by flow cytometry. The CSF levels of total tau (t-tau), phosphorylated tau (p-tau), and amyloid-β (Aβ)42 were determined using ELISA. CSF Aβ-oligomer levels in AD patients were elevated in comparison to the non-AD group (p = 0.073). The ratio Aβ-oligomers/Aβ42 was significantly elevated in AD subjects compared to non-AD subjects (p = 0.001). Most important, there was a negative correlation between the amount of Aβ-oligomers and the Mini-Mental Status Exam score (r = -0.65; p = 0.013) in AD patients. The detection of Aβ-oligomers using flow cytometry analysis seems to be useful in assessing the stage of AD. This is a novel and important finding as none of the currently used CSF biomarkers are clearly associated with dementia severity. SN - 1875-8908 UR - https://www.unboundmedicine.com/medline/citation/22214781/Amyloid_β_oligomers_in_cerebrospinal_fluid_are_associated_with_cognitive_decline_in_patients_with_Alzheimer's_disease_ L2 - https://content.iospress.com/openurl?genre=article&id=doi:10.3233/JAD-2012-111361 DB - PRIME DP - Unbound Medicine ER -