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Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples.
J Microbiol Methods. 2012 Feb; 88(2):304-10.JM

Abstract

The purpose of this study was to examine the performance of novel agars for the identification and enumeration of Campylobacter species. The analytical sensitivity and specificity of Campylobacter Selective agar (CASA), Brilliance CampyCount agar (BCCA) and CampyFoodIDagar (CFA) for 84 Campylobacter spp. isolates and 50 non-Campylobacter spp. isolates from 37 distinct genera were of 100% sensitivity, with a 98% specificity for BCCA and CFA, and a 100% specificity for CASA. The application of these selective agars for Campylobacter spp. enumeration in comparison to the conventional agars, modified charcoal cefoperazonedeoxycholate agar (mCCDA) and Campy-Cefex (CCA) was examined using Campylobacter jejuni and Campylobacter coli inoculated samples. From C. jejuni inoculated samples, recovery on BCCA was significantly greater than other media (p<0.05). Recovery on CASA was not significantly different from mCCDA and CCA (p>0.05). With C. coli inoculated samples, recovery was significantly greater on BCCA and CASA than with other media (p<0.05). The recovery of both C. jejuni and C. coli from inoculated samples with CFA was significantly less than with other media (P<0.05). CASA was able to effectively inhibit and differentiate Campylobacter spp. from background microflora while false positive organisms occurred with BCCA and CFA. An examination of 483 randomly selected suspect Campylobacter colonies from naturally contaminated samples demonstrated a colony confirmation rate for CCA, CFA, BCCA, mCCDA, and CASA, of 84%, 87%, 88%, 90%, and 100%, respectively. The media evaluated present an alternative to conventional selective agars for the identification and enumeration of thermotolerant Campylobacter spp. from samples of poultry origin through the farm to fork continuum.

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Authors+Show Affiliations

Ahmed R
Laboratory Services Division, University of Guelph, Ontario, Canada.
León-Velarde CG
No affiliation info available
Odumeru JA
No affiliation info available

MeSH

AgarAnimalsCampylobacterChickensColony Count, MicrobialCulture MediaFood ContaminationMeatSensitivity and Specificity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22226753

Citation

Ahmed, R, et al. "Evaluation of Novel Agars for the Enumeration of Campylobacter Spp. in Poultry Retail Samples." Journal of Microbiological Methods, vol. 88, no. 2, 2012, pp. 304-10.
Ahmed R, León-Velarde CG, Odumeru JA. Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples. J Microbiol Methods. 2012;88(2):304-10.
Ahmed, R., León-Velarde, C. G., & Odumeru, J. A. (2012). Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples. Journal of Microbiological Methods, 88(2), 304-10. https://doi.org/10.1016/j.mimet.2011.12.011
Ahmed R, León-Velarde CG, Odumeru JA. Evaluation of Novel Agars for the Enumeration of Campylobacter Spp. in Poultry Retail Samples. J Microbiol Methods. 2012;88(2):304-10. PubMed PMID: 22226753.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples. AU - Ahmed,R, AU - León-Velarde,C G, AU - Odumeru,J A, Y1 - 2011/12/23/ PY - 2011/11/13/received PY - 2011/12/18/accepted PY - 2012/1/10/entrez PY - 2012/1/10/pubmed PY - 2012/8/15/medline SP - 304 EP - 10 JF - Journal of microbiological methods JO - J Microbiol Methods VL - 88 IS - 2 N2 - The purpose of this study was to examine the performance of novel agars for the identification and enumeration of Campylobacter species. The analytical sensitivity and specificity of Campylobacter Selective agar (CASA), Brilliance CampyCount agar (BCCA) and CampyFoodIDagar (CFA) for 84 Campylobacter spp. isolates and 50 non-Campylobacter spp. isolates from 37 distinct genera were of 100% sensitivity, with a 98% specificity for BCCA and CFA, and a 100% specificity for CASA. The application of these selective agars for Campylobacter spp. enumeration in comparison to the conventional agars, modified charcoal cefoperazonedeoxycholate agar (mCCDA) and Campy-Cefex (CCA) was examined using Campylobacter jejuni and Campylobacter coli inoculated samples. From C. jejuni inoculated samples, recovery on BCCA was significantly greater than other media (p<0.05). Recovery on CASA was not significantly different from mCCDA and CCA (p>0.05). With C. coli inoculated samples, recovery was significantly greater on BCCA and CASA than with other media (p<0.05). The recovery of both C. jejuni and C. coli from inoculated samples with CFA was significantly less than with other media (P<0.05). CASA was able to effectively inhibit and differentiate Campylobacter spp. from background microflora while false positive organisms occurred with BCCA and CFA. An examination of 483 randomly selected suspect Campylobacter colonies from naturally contaminated samples demonstrated a colony confirmation rate for CCA, CFA, BCCA, mCCDA, and CASA, of 84%, 87%, 88%, 90%, and 100%, respectively. The media evaluated present an alternative to conventional selective agars for the identification and enumeration of thermotolerant Campylobacter spp. from samples of poultry origin through the farm to fork continuum. SN - 1872-8359 UR - https://www.unboundmedicine.com/medline/citation/22226753/Evaluation_of_novel_agars_for_the_enumeration_of_Campylobacter_spp__in_poultry_retail_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-7012(11)00437-4 DB - PRIME DP - Unbound Medicine ER -