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Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum).
Mol Genet Genomics. 2012 Mar; 287(3):221-35.MG

Abstract

In plants, auxin-mediated responses are regulated by diverse proteins. One such class of proteins, i.e. GH3, is involved in the conjugation of IAA to amino acids and provides a negative feedback loop to control auxin homoeostasis. In order to have a better understanding of the mechanism of the auxin action, 15 genes encoding GH3 members were identified using existing EST databases of tomato. Their orthologs were identified from tobacco, potato, N. benthemiana, pepper, and petunia. Phylogenetic analysis of AtGH3, SlGH3, and their Solanaceae orthologs provided insights into various orthologous relationships among these proteins. These genes were found to be responsive to a variety of signals including, phytohormones and environmental stresses. Analysis of AuxRE elements in their promoters showed variability in the sequence as well as number of this element. Up-regulation of only 11 SlGH3 genes, in response to exogenous auxin, suggested possible relationship between the diversity in the sequence and number of AuxRE element with the auxin inducibility. Expression analysis of SlGH3 genes in different vegetative and reproductive tissues/stages suggested limited or no role for most of the SlGH3 genes at the initiation of fruit ripening. However, up-regulation of SlGH3-1 and -2 at the onset of fruit ripening indicates that these genes could have a role in fruit ripening. The present study characterizes GH3 gene family of tomato and its evolutionary relationship with members of this family from other Solanaceae species and Arabidopsis. It could help in the identification of GH3 genes and revelation of their function during vegetative/reproductive development stages from other Solanaceae members.

Authors+Show Affiliations

Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, 110021, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22228229

Citation

Kumar, Rahul, et al. "Genome-wide Investigation and Expression Analysis Suggest Diverse Roles of Auxin-responsive GH3 Genes During Development and Response to Different Stimuli in Tomato (Solanum Lycopersicum)." Molecular Genetics and Genomics : MGG, vol. 287, no. 3, 2012, pp. 221-35.
Kumar R, Agarwal P, Tyagi AK, et al. Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum). Mol Genet Genomics. 2012;287(3):221-35.
Kumar, R., Agarwal, P., Tyagi, A. K., & Sharma, A. K. (2012). Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum). Molecular Genetics and Genomics : MGG, 287(3), 221-35. https://doi.org/10.1007/s00438-011-0672-6
Kumar R, et al. Genome-wide Investigation and Expression Analysis Suggest Diverse Roles of Auxin-responsive GH3 Genes During Development and Response to Different Stimuli in Tomato (Solanum Lycopersicum). Mol Genet Genomics. 2012;287(3):221-35. PubMed PMID: 22228229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 genes during development and response to different stimuli in tomato (Solanum lycopersicum). AU - Kumar,Rahul, AU - Agarwal,Priyanka, AU - Tyagi,Akhilesh K, AU - Sharma,Arun K, Y1 - 2012/01/08/ PY - 2011/10/13/received PY - 2011/12/22/accepted PY - 2012/1/10/entrez PY - 2012/1/10/pubmed PY - 2012/7/6/medline SP - 221 EP - 35 JF - Molecular genetics and genomics : MGG JO - Mol Genet Genomics VL - 287 IS - 3 N2 - In plants, auxin-mediated responses are regulated by diverse proteins. One such class of proteins, i.e. GH3, is involved in the conjugation of IAA to amino acids and provides a negative feedback loop to control auxin homoeostasis. In order to have a better understanding of the mechanism of the auxin action, 15 genes encoding GH3 members were identified using existing EST databases of tomato. Their orthologs were identified from tobacco, potato, N. benthemiana, pepper, and petunia. Phylogenetic analysis of AtGH3, SlGH3, and their Solanaceae orthologs provided insights into various orthologous relationships among these proteins. These genes were found to be responsive to a variety of signals including, phytohormones and environmental stresses. Analysis of AuxRE elements in their promoters showed variability in the sequence as well as number of this element. Up-regulation of only 11 SlGH3 genes, in response to exogenous auxin, suggested possible relationship between the diversity in the sequence and number of AuxRE element with the auxin inducibility. Expression analysis of SlGH3 genes in different vegetative and reproductive tissues/stages suggested limited or no role for most of the SlGH3 genes at the initiation of fruit ripening. However, up-regulation of SlGH3-1 and -2 at the onset of fruit ripening indicates that these genes could have a role in fruit ripening. The present study characterizes GH3 gene family of tomato and its evolutionary relationship with members of this family from other Solanaceae species and Arabidopsis. It could help in the identification of GH3 genes and revelation of their function during vegetative/reproductive development stages from other Solanaceae members. SN - 1617-4623 UR - https://www.unboundmedicine.com/medline/citation/22228229/Genome_wide_investigation_and_expression_analysis_suggest_diverse_roles_of_auxin_responsive_GH3_genes_during_development_and_response_to_different_stimuli_in_tomato__Solanum_lycopersicum__ L2 - https://doi.org/10.1007/s00438-011-0672-6 DB - PRIME DP - Unbound Medicine ER -