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Microarray analysis of global gene regulation in the Cry1Ab-resistant and Cry1Ab-susceptible strains of Diatraea saccharalis.
Pest Manag Sci. 2012 May; 68(5):718-30.PM

Abstract

BACKGROUND

Extensive adoption of transgenic Bt corn in recent years for stalk borer control has increased risk of resistance evolution in the target pest populations. A Bt-resistant strain of the sugarcane borer, Diatraea saccharalis, was approximately 100-fold more tolerant to Cry1Ab toxin than the susceptible counterpart. To gain a better understanding of the molecular mechanisms of Bt resistance, the Cry1Ab-susceptible (Cry1Ab-SS) and Cry1Ab-resistant (Cry1Ab-RR) strains of D. saccharalis were subjected to a microarray analysis.

RESULTS

Results showed that the expression levels of many genes were significantly different between the Cry1Ab-RR and Cry1Ab-SS strains. Microarray analysis of 7145 cDNAs revealed 384 differentially expressed genes. A total of 273 genes were significantly upregulated 2-51.6-fold, and 111 genes were significantly downregulated 2-22.6-fold in the Cry1Ab-RR strain. The upregulation of three potential resistance-related genes, coding for a glutathione S-transferase (GST), a chymotrypsin-like protease (CHY) and a lipase (LP), was confirmed using real-time PCR, indicating a reproducibility of the microarray data. Ontology analysis revealed that more than twice the number of metabolic-related genes were upregulated compared with downregulated genes with the same biological function. Up to 35.2% of the upregulated genes in the resistant strain were associated with catalytic activity, while only 9.5% of the downregulated genes were related to the same catalytic molecular function.

CONCLUSION

The large portion of metabolic- or catalytic-related genes with significant upregulations indicated a potential large increase in metabolic or catalytic activities in the Cry1Ab-RR strain. This cDNA microarray gene expression data could be used to characterize and identify new genes that may be associated with Bt resistance in D. saccharalis.

Authors+Show Affiliations

SIMRU, ARS-USDA, Stoneville, MS, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22228544

Citation

Guo, Zibiao, et al. "Microarray Analysis of Global Gene Regulation in the Cry1Ab-resistant and Cry1Ab-susceptible Strains of Diatraea Saccharalis." Pest Management Science, vol. 68, no. 5, 2012, pp. 718-30.
Guo Z, Cheng Zhu Y, Huang F, et al. Microarray analysis of global gene regulation in the Cry1Ab-resistant and Cry1Ab-susceptible strains of Diatraea saccharalis. Pest Manag Sci. 2012;68(5):718-30.
Guo, Z., Cheng Zhu, Y., Huang, F., Luttrell, R., & Leonard, R. (2012). Microarray analysis of global gene regulation in the Cry1Ab-resistant and Cry1Ab-susceptible strains of Diatraea saccharalis. Pest Management Science, 68(5), 718-30. https://doi.org/10.1002/ps.2318
Guo Z, et al. Microarray Analysis of Global Gene Regulation in the Cry1Ab-resistant and Cry1Ab-susceptible Strains of Diatraea Saccharalis. Pest Manag Sci. 2012;68(5):718-30. PubMed PMID: 22228544.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microarray analysis of global gene regulation in the Cry1Ab-resistant and Cry1Ab-susceptible strains of Diatraea saccharalis. AU - Guo,Zibiao, AU - Cheng Zhu,Yu, AU - Huang,Fangneng, AU - Luttrell,Randall, AU - Leonard,Rogers, Y1 - 2012/01/06/ PY - 2011/05/29/received PY - 2011/08/17/revised PY - 2011/09/13/accepted PY - 2012/1/10/entrez PY - 2012/1/10/pubmed PY - 2012/7/31/medline SP - 718 EP - 30 JF - Pest management science JO - Pest Manag Sci VL - 68 IS - 5 N2 - BACKGROUND: Extensive adoption of transgenic Bt corn in recent years for stalk borer control has increased risk of resistance evolution in the target pest populations. A Bt-resistant strain of the sugarcane borer, Diatraea saccharalis, was approximately 100-fold more tolerant to Cry1Ab toxin than the susceptible counterpart. To gain a better understanding of the molecular mechanisms of Bt resistance, the Cry1Ab-susceptible (Cry1Ab-SS) and Cry1Ab-resistant (Cry1Ab-RR) strains of D. saccharalis were subjected to a microarray analysis. RESULTS: Results showed that the expression levels of many genes were significantly different between the Cry1Ab-RR and Cry1Ab-SS strains. Microarray analysis of 7145 cDNAs revealed 384 differentially expressed genes. A total of 273 genes were significantly upregulated 2-51.6-fold, and 111 genes were significantly downregulated 2-22.6-fold in the Cry1Ab-RR strain. The upregulation of three potential resistance-related genes, coding for a glutathione S-transferase (GST), a chymotrypsin-like protease (CHY) and a lipase (LP), was confirmed using real-time PCR, indicating a reproducibility of the microarray data. Ontology analysis revealed that more than twice the number of metabolic-related genes were upregulated compared with downregulated genes with the same biological function. Up to 35.2% of the upregulated genes in the resistant strain were associated with catalytic activity, while only 9.5% of the downregulated genes were related to the same catalytic molecular function. CONCLUSION: The large portion of metabolic- or catalytic-related genes with significant upregulations indicated a potential large increase in metabolic or catalytic activities in the Cry1Ab-RR strain. This cDNA microarray gene expression data could be used to characterize and identify new genes that may be associated with Bt resistance in D. saccharalis. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/22228544/Microarray_analysis_of_global_gene_regulation_in_the_Cry1Ab_resistant_and_Cry1Ab_susceptible_strains_of_Diatraea_saccharalis_ L2 - https://doi.org/10.1002/ps.2318 DB - PRIME DP - Unbound Medicine ER -