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Extracellular matrix in airway smooth muscle is associated with dynamics of airway function in asthma.
Allergy. 2012 Apr; 67(4):552-9.A

Abstract

BACKGROUND

Altered deposition of extracellular matrix (ECM) in the airway smooth muscle (ASM) layer as observed in asthma may influence ASM mechanical properties. We hypothesized that ECM in ASM is associated with airway function in asthma. First, we investigated the difference in ECM expression in ASM between asthma and controls. Second, we examined whether ECM expression is associated with bronchoconstriction and bronchodilation in vivo.

METHODS

Our cross-sectional study comprised 19 atopic mild asthma patients, 15 atopic and 12 nonatopic healthy subjects. Spirometry, methacholine responsiveness, deep-breath-induced bronchodilation (ΔR(rs)) and bronchoscopy with endobronchial biopsies were performed. Positive staining of elastin, collagen I, III and IV, decorin, versican, fibronectin, laminin and tenascin in ASM was quantified as fractional area and mean density. Data were analysed using Pearson's or Spearman's correlation coefficient.

RESULTS

Extracellular matrix expression in ASM was not different between asthma and controls. In asthmatics, fractional area and mean density of collagen I and III were correlated with methacholine dose-response slope and ΔR(rs) , respectively (r = 0.71, P < 0.01; r = 0.60, P = 0.02). Furthermore, ASM collagen III and laminin in asthma were correlated with FEV(1) reversibility (r = -0.65, P = 0.01; r = -0.54, P = 0.04).

CONCLUSION

In asthma, ECM in ASM is related to the dynamics of airway function in the absence of differences in ECM expression between asthma and controls. This indicates that the ASM layer in its full composition is a major structural component in determining variable airways obstruction in asthma.

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Authors+Show Affiliations

Yick CY
Department of Respiratory Medicine, Academic Medical Centre, Amsterdam, the Netherlands. c.y.yick@amc.uva.nl
Ferreira DS
No affiliation info available
Annoni R
No affiliation info available
von der Thüsen JH
No affiliation info available
Kunst PW
No affiliation info available
Bel EH
No affiliation info available
Lutter R
No affiliation info available
Mauad T
No affiliation info available
Sterk PJ
No affiliation info available

MeSH

AdultAsthmaBronchiBronchoscopyCollagenCross-Sectional StudiesExtracellular MatrixFemaleHumansImmunohistochemistryMaleMiddle AgedMuscle, SmoothRespiratory Function TestsYoung Adult

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22229658

Citation

Yick, C Y., et al. "Extracellular Matrix in Airway Smooth Muscle Is Associated With Dynamics of Airway Function in Asthma." Allergy, vol. 67, no. 4, 2012, pp. 552-9.
Yick CY, Ferreira DS, Annoni R, et al. Extracellular matrix in airway smooth muscle is associated with dynamics of airway function in asthma. Allergy. 2012;67(4):552-9.
Yick, C. Y., Ferreira, D. S., Annoni, R., von der Thüsen, J. H., Kunst, P. W., Bel, E. H., Lutter, R., Mauad, T., & Sterk, P. J. (2012). Extracellular matrix in airway smooth muscle is associated with dynamics of airway function in asthma. Allergy, 67(4), 552-9. https://doi.org/10.1111/j.1398-9995.2011.02773.x
Yick CY, et al. Extracellular Matrix in Airway Smooth Muscle Is Associated With Dynamics of Airway Function in Asthma. Allergy. 2012;67(4):552-9. PubMed PMID: 22229658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extracellular matrix in airway smooth muscle is associated with dynamics of airway function in asthma. AU - Yick,C Y, AU - Ferreira,D S, AU - Annoni,R, AU - von der Thüsen,J H, AU - Kunst,P W, AU - Bel,E H, AU - Lutter,R, AU - Mauad,T, AU - Sterk,P J, Y1 - 2012/01/09/ PY - 2011/11/26/accepted PY - 2012/1/11/entrez PY - 2012/1/11/pubmed PY - 2012/6/26/medline SP - 552 EP - 9 JF - Allergy JO - Allergy VL - 67 IS - 4 N2 - BACKGROUND: Altered deposition of extracellular matrix (ECM) in the airway smooth muscle (ASM) layer as observed in asthma may influence ASM mechanical properties. We hypothesized that ECM in ASM is associated with airway function in asthma. First, we investigated the difference in ECM expression in ASM between asthma and controls. Second, we examined whether ECM expression is associated with bronchoconstriction and bronchodilation in vivo. METHODS: Our cross-sectional study comprised 19 atopic mild asthma patients, 15 atopic and 12 nonatopic healthy subjects. Spirometry, methacholine responsiveness, deep-breath-induced bronchodilation (ΔR(rs)) and bronchoscopy with endobronchial biopsies were performed. Positive staining of elastin, collagen I, III and IV, decorin, versican, fibronectin, laminin and tenascin in ASM was quantified as fractional area and mean density. Data were analysed using Pearson's or Spearman's correlation coefficient. RESULTS: Extracellular matrix expression in ASM was not different between asthma and controls. In asthmatics, fractional area and mean density of collagen I and III were correlated with methacholine dose-response slope and ΔR(rs) , respectively (r = 0.71, P < 0.01; r = 0.60, P = 0.02). Furthermore, ASM collagen III and laminin in asthma were correlated with FEV(1) reversibility (r = -0.65, P = 0.01; r = -0.54, P = 0.04). CONCLUSION: In asthma, ECM in ASM is related to the dynamics of airway function in the absence of differences in ECM expression between asthma and controls. This indicates that the ASM layer in its full composition is a major structural component in determining variable airways obstruction in asthma. SN - 1398-9995 UR - https://www.unboundmedicine.com/medline/citation/22229658/Extracellular_matrix_in_airway_smooth_muscle_is_associated_with_dynamics_of_airway_function_in_asthma_ L2 - https://doi.org/10.1111/j.1398-9995.2011.02773.x DB - PRIME DP - Unbound Medicine ER -