Tags

Type your tag names separated by a space and hit enter

Plumbagin inhibits cell growth and potentiates apoptosis in human gastric cancer cells in vitro through the NF-κB signaling pathway.
Acta Pharmacol Sin. 2012 Feb; 33(2):242-9.AP

Abstract

AIM

To investigate the effects and underlying mechanisms of plumbagin, a naphthoquinone derived from medicinal plant Plumbago zeylanica, on human gastric cancer (GC) cells.

METHODS

Human gastric cancer cell lines SGC-7901, MKN-28, and AGS were used. The cell viability was examined using CCK-8 viability assay. Cell proliferation rate was determined using both clonogenic assay and EdU incorporation assay. Apoptosis was detected via Annexin V/propidium iodide double-labeled flow cytometry. Western blotting was used to assess the expression of both NF-κB-regulated gene products and TNF-α-induced activation of p65, IκBα, and IKK. The intracellular location of NF-κB p65 was detected using confocal microscopy.

RESULTS

Plumbagin (2.5-40 μmol/L) concentration-dependently reduced the viability of the GC cells. The IC(50) value of plumbagin in SGC-7901, MKN-28, and AGS cells was 19.12, 13.64, and 10.12 μmol/L, respectively. The compound (5-20 μmol/L) concentration-dependently induced apoptosis of SGC-7901 cells, and potentiated the sensitivity of SGC-7901 cells to chemotherapeutic agents TNF-αand cisplatin. The compound (10 μmol/L) downregulated the expression of NF-κB-regulated gene products, including IAP1, XIAP, Bcl-2, Bcl-xL, tumor factor (TF), and VEGF. In addition to inhibition of NF-κB p65 nuclear translocation, the compound also suppressed TNF-α-induced phosphorylation of p65 and IKK, and the degradation of IκBα.

CONCLUSION

Plumbagin inhibits cell growth and potentiates apoptosis in human GC cells through the NF-κB pathway.

Authors+Show Affiliations

Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22231395

Citation

Li, Jing, et al. "Plumbagin Inhibits Cell Growth and Potentiates Apoptosis in Human Gastric Cancer Cells in Vitro Through the NF-κB Signaling Pathway." Acta Pharmacologica Sinica, vol. 33, no. 2, 2012, pp. 242-9.
Li J, Shen L, Lu FR, et al. Plumbagin inhibits cell growth and potentiates apoptosis in human gastric cancer cells in vitro through the NF-κB signaling pathway. Acta Pharmacol Sin. 2012;33(2):242-9.
Li, J., Shen, L., Lu, F. R., Qin, Y., Chen, R., Li, J., Li, Y., Zhan, H. Z., & He, Y. Q. (2012). Plumbagin inhibits cell growth and potentiates apoptosis in human gastric cancer cells in vitro through the NF-κB signaling pathway. Acta Pharmacologica Sinica, 33(2), 242-9. https://doi.org/10.1038/aps.2011.152
Li J, et al. Plumbagin Inhibits Cell Growth and Potentiates Apoptosis in Human Gastric Cancer Cells in Vitro Through the NF-κB Signaling Pathway. Acta Pharmacol Sin. 2012;33(2):242-9. PubMed PMID: 22231395.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plumbagin inhibits cell growth and potentiates apoptosis in human gastric cancer cells in vitro through the NF-κB signaling pathway. AU - Li,Jing, AU - Shen,Lin, AU - Lu,Fu-rong, AU - Qin,You, AU - Chen,Rui, AU - Li,Jia, AU - Li,Yan, AU - Zhan,Han-zi, AU - He,Yuan-qiao, Y1 - 2012/01/09/ PY - 2012/1/11/entrez PY - 2012/1/11/pubmed PY - 2012/5/26/medline SP - 242 EP - 9 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 33 IS - 2 N2 - AIM: To investigate the effects and underlying mechanisms of plumbagin, a naphthoquinone derived from medicinal plant Plumbago zeylanica, on human gastric cancer (GC) cells. METHODS: Human gastric cancer cell lines SGC-7901, MKN-28, and AGS were used. The cell viability was examined using CCK-8 viability assay. Cell proliferation rate was determined using both clonogenic assay and EdU incorporation assay. Apoptosis was detected via Annexin V/propidium iodide double-labeled flow cytometry. Western blotting was used to assess the expression of both NF-κB-regulated gene products and TNF-α-induced activation of p65, IκBα, and IKK. The intracellular location of NF-κB p65 was detected using confocal microscopy. RESULTS: Plumbagin (2.5-40 μmol/L) concentration-dependently reduced the viability of the GC cells. The IC(50) value of plumbagin in SGC-7901, MKN-28, and AGS cells was 19.12, 13.64, and 10.12 μmol/L, respectively. The compound (5-20 μmol/L) concentration-dependently induced apoptosis of SGC-7901 cells, and potentiated the sensitivity of SGC-7901 cells to chemotherapeutic agents TNF-αand cisplatin. The compound (10 μmol/L) downregulated the expression of NF-κB-regulated gene products, including IAP1, XIAP, Bcl-2, Bcl-xL, tumor factor (TF), and VEGF. In addition to inhibition of NF-κB p65 nuclear translocation, the compound also suppressed TNF-α-induced phosphorylation of p65 and IKK, and the degradation of IκBα. CONCLUSION: Plumbagin inhibits cell growth and potentiates apoptosis in human GC cells through the NF-κB pathway. SN - 1745-7254 UR - https://www.unboundmedicine.com/medline/citation/22231395/Plumbagin_inhibits_cell_growth_and_potentiates_apoptosis_in_human_gastric_cancer_cells_in_vitro_through_the_NF_κB_signaling_pathway_ L2 - https://doi.org/10.1038/aps.2011.152 DB - PRIME DP - Unbound Medicine ER -