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Ferric Iron and Cobalt (III) compounds to safely decrease hydrogen sulfide in the body?
Antioxid Redox Signal 2013; 19(5):510-6AR

Abstract

To sort out the putative roles of endogenous hydrogen sulfide (H2S) in clinical conditions wherein systemic inflammation or hypoxia is present, it becomes crucial to develop approaches capable of affecting H2S concentration that can be safely applied in humans. We have investigated a paradigm, which could achieve such a goal, using vitamin B12 (vit.B12), at the dose recommended in cyanide poisoning, and very low levels of methemoglobin (MetHb). Hydroxocobalamin in the plasma, supernatant of kidney, and heart tissue homogenates of rats that had received vit.B12 (140 mg.kg(-1) intravenous) was found in the μM range. Exogenous H2S (100 μM) added to the plasma or supernatants of these rats decreased at a significantly higher rate than in control rats. In the latter however a spontaneous oxidation of exogenous H2S occurred. In vitro, hydroxocobalamin solution (100 μM) decreased, within <2 min, an equimolar concentration of H2S by 80%. Three to five percent MetHb prevented H2S induced hyperventilation in vivo and decreased exogenous H2S in vitro by 25-40 μM within 30 s. Our observations lead to the hypothesis that innocuous levels of MetHb and vit.B12 could be a used as an effective and safe way to test the role of endogenous H2S in vivo.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

News

Language

eng

PubMed ID

22233239

Citation

Van de Louw, Andry, and Philippe Haouzi. "Ferric Iron and Cobalt (III) Compounds to Safely Decrease Hydrogen Sulfide in the Body?" Antioxidants & Redox Signaling, vol. 19, no. 5, 2013, pp. 510-6.
Van de Louw A, Haouzi P. Ferric Iron and Cobalt (III) compounds to safely decrease hydrogen sulfide in the body? Antioxid Redox Signal. 2013;19(5):510-6.
Van de Louw, A., & Haouzi, P. (2013). Ferric Iron and Cobalt (III) compounds to safely decrease hydrogen sulfide in the body? Antioxidants & Redox Signaling, 19(5), pp. 510-6. doi:10.1089/ars.2012.4513.
Van de Louw A, Haouzi P. Ferric Iron and Cobalt (III) Compounds to Safely Decrease Hydrogen Sulfide in the Body. Antioxid Redox Signal. 2013 Aug 10;19(5):510-6. PubMed PMID: 22233239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ferric Iron and Cobalt (III) compounds to safely decrease hydrogen sulfide in the body? AU - Van de Louw,Andry, AU - Haouzi,Philippe, Y1 - 2012/03/06/ PY - 2012/1/12/entrez PY - 2012/1/12/pubmed PY - 2014/2/1/medline SP - 510 EP - 6 JF - Antioxidants & redox signaling JO - Antioxid. Redox Signal. VL - 19 IS - 5 N2 - To sort out the putative roles of endogenous hydrogen sulfide (H2S) in clinical conditions wherein systemic inflammation or hypoxia is present, it becomes crucial to develop approaches capable of affecting H2S concentration that can be safely applied in humans. We have investigated a paradigm, which could achieve such a goal, using vitamin B12 (vit.B12), at the dose recommended in cyanide poisoning, and very low levels of methemoglobin (MetHb). Hydroxocobalamin in the plasma, supernatant of kidney, and heart tissue homogenates of rats that had received vit.B12 (140 mg.kg(-1) intravenous) was found in the μM range. Exogenous H2S (100 μM) added to the plasma or supernatants of these rats decreased at a significantly higher rate than in control rats. In the latter however a spontaneous oxidation of exogenous H2S occurred. In vitro, hydroxocobalamin solution (100 μM) decreased, within <2 min, an equimolar concentration of H2S by 80%. Three to five percent MetHb prevented H2S induced hyperventilation in vivo and decreased exogenous H2S in vitro by 25-40 μM within 30 s. Our observations lead to the hypothesis that innocuous levels of MetHb and vit.B12 could be a used as an effective and safe way to test the role of endogenous H2S in vivo. SN - 1557-7716 UR - https://www.unboundmedicine.com/medline/citation/22233239/Ferric_Iron_and_Cobalt__III__compounds_to_safely_decrease_hydrogen_sulfide_in_the_body L2 - https://www.liebertpub.com/doi/full/10.1089/ars.2012.4513?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -