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Involvement of microglia and interleukin-18 in the induction of long-term potentiation of spinal nociceptive responses induced by tetanic sciatic stimulation.
Neurosci Bull. 2012 Feb; 28(1):49-60.NB

Abstract

OBJECTIVE

The present study aimed to investigate the potential roles of spinal microglia and downstream molecules in the induction of spinal long-term potentiation (LTP) and mechanical allodynia by tetanic stimulation of the sciatic nerve (TSS).

METHODS

Spinal LTP was induced in adult male Sprague-Dawley rats by tetanic stimulation of the sciatic nerve (0.5 ms, 100 Hz, 40 V, 10 trains of 2-s duration at 10-s intervals). Mechanical allodynia was determined using von Frey hairs. Immunohistochemical staining and Western blot were used to detect changes in glial expression of interleukin-18 (IL-18) and IL-18 receptor (IL-18R).

RESULTS

TSS induced LTP of C-fiber-evoked field potentials in the spinal cord. Intrathecal administration of the microglial inhibitor minocycline (200 μg/20 μL) 1 h before TSS completely blocked the induction of spinal LTP. Furthermore, after intrathecal injection of minocycline (200 μg/20 μL) by lumbar puncture 1 h before TSS, administration of minocycline for 7 consecutive days (once per day) partly inhibited bilateral allodynia. Immunohistochemistry showed that minocycline inhibited the sequential activation of microglia and astrocytes, and IL-18 was predominantly colocalized with the microglial marker Iba-1 in the spinal superficial dorsal horn. Western blot revealed that repeated intrathecal injection of minocycline significantly inhibited the increased expression of IL-18 and IL-18Rs in microglia induced by TSS.

CONCLUSION

The IL-18 signaling pathway in microglia is involved in TSS-induced spinal LTP and mechanical allodynia.

Authors+Show Affiliations

Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22233889

Citation

Chu, Yu-Xia, et al. "Involvement of Microglia and Interleukin-18 in the Induction of Long-term Potentiation of Spinal Nociceptive Responses Induced By Tetanic Sciatic Stimulation." Neuroscience Bulletin, vol. 28, no. 1, 2012, pp. 49-60.
Chu YX, Zhang YQ, Zhao ZQ. Involvement of microglia and interleukin-18 in the induction of long-term potentiation of spinal nociceptive responses induced by tetanic sciatic stimulation. Neurosci Bull. 2012;28(1):49-60.
Chu, Y. X., Zhang, Y. Q., & Zhao, Z. Q. (2012). Involvement of microglia and interleukin-18 in the induction of long-term potentiation of spinal nociceptive responses induced by tetanic sciatic stimulation. Neuroscience Bulletin, 28(1), 49-60. https://doi.org/10.1007/s12264-012-1058-4
Chu YX, Zhang YQ, Zhao ZQ. Involvement of Microglia and Interleukin-18 in the Induction of Long-term Potentiation of Spinal Nociceptive Responses Induced By Tetanic Sciatic Stimulation. Neurosci Bull. 2012;28(1):49-60. PubMed PMID: 22233889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of microglia and interleukin-18 in the induction of long-term potentiation of spinal nociceptive responses induced by tetanic sciatic stimulation. AU - Chu,Yu-Xia, AU - Zhang,Yu-Qiu, AU - Zhao,Zhi-Qi, PY - 2012/1/12/entrez PY - 2012/1/12/pubmed PY - 2012/5/5/medline SP - 49 EP - 60 JF - Neuroscience bulletin JO - Neurosci Bull VL - 28 IS - 1 N2 - OBJECTIVE: The present study aimed to investigate the potential roles of spinal microglia and downstream molecules in the induction of spinal long-term potentiation (LTP) and mechanical allodynia by tetanic stimulation of the sciatic nerve (TSS). METHODS: Spinal LTP was induced in adult male Sprague-Dawley rats by tetanic stimulation of the sciatic nerve (0.5 ms, 100 Hz, 40 V, 10 trains of 2-s duration at 10-s intervals). Mechanical allodynia was determined using von Frey hairs. Immunohistochemical staining and Western blot were used to detect changes in glial expression of interleukin-18 (IL-18) and IL-18 receptor (IL-18R). RESULTS: TSS induced LTP of C-fiber-evoked field potentials in the spinal cord. Intrathecal administration of the microglial inhibitor minocycline (200 μg/20 μL) 1 h before TSS completely blocked the induction of spinal LTP. Furthermore, after intrathecal injection of minocycline (200 μg/20 μL) by lumbar puncture 1 h before TSS, administration of minocycline for 7 consecutive days (once per day) partly inhibited bilateral allodynia. Immunohistochemistry showed that minocycline inhibited the sequential activation of microglia and astrocytes, and IL-18 was predominantly colocalized with the microglial marker Iba-1 in the spinal superficial dorsal horn. Western blot revealed that repeated intrathecal injection of minocycline significantly inhibited the increased expression of IL-18 and IL-18Rs in microglia induced by TSS. CONCLUSION: The IL-18 signaling pathway in microglia is involved in TSS-induced spinal LTP and mechanical allodynia. SN - 1995-8218 UR - https://www.unboundmedicine.com/medline/citation/22233889/Involvement_of_microglia_and_interleukin_18_in_the_induction_of_long_term_potentiation_of_spinal_nociceptive_responses_induced_by_tetanic_sciatic_stimulation_ L2 - https://dx.doi.org/10.1007/s12264-012-1058-4 DB - PRIME DP - Unbound Medicine ER -