Tags

Type your tag names separated by a space and hit enter

Usefulness of combined in vivo skin comet assay and in vivo skin micronucleus test.
Mutat Res. 2012 Mar 18; 743(1-2):42-51.MR

Abstract

We have already found that the in vivo skin comet assay is useful for the evaluation of primary DNA damage induced by genotoxic chemicals in epidermal skin cells. The aim of the present study was to evaluate the sensitivity and specificity of the combined in vivo skin comet assay and in vivo skin micronucleus (MN) test using the same animal to explore the usefulness of the new test method. The combined alkaline comet assay and MN test was carried out with three chemicals: 4-nitroquinoline-1-oxide (4NQO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and benzo[a]pyrene (B[a]P). In the first experiment, we compared DNA- and chromosome-damaging effects of 3 [72, 24 and 3 hours (h) before sacrifice] and 4 applications (72, 48, 24 and 3h before sacrifice) of 4NQO, which induces dermal irritancy. The animals were euthanized and their skin was sampled for the combination test. As a result, the 4-application method was able to detect both DNA- and chromosome-damaging potential with a lower concentration; therefore, in the second experiment, MNNG and B[a]P were topically applied four times, respectively. The animals were euthanized, and then their skins were sampled for combination tests. In the alkaline comet assay, significant differences in the percent of DNA (%DNA) in the tail were observed in epidermal skin cells treated with MNNG and B[a]P. In the MN test, an increased frequency of MN cells (%MN) cells was observed by treatment with MNNG; however, there were no significant increases. In contrast, significant differences in %MN were observed by treatment with B[a]P. From these results, we conclude that the combined in vivo skin comet assay and in vivo MN test was useful because it can detect different genotoxicity with the same sampling time and reduce the number of animals used.

Authors+Show Affiliations

Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano, Kanagawa 257-8523, Japan. toyoizumi.t@fdsc.or.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22240394

Citation

Toyoizumi, Tomoyasu, et al. "Usefulness of Combined in Vivo Skin Comet Assay and in Vivo Skin Micronucleus Test." Mutation Research, vol. 743, no. 1-2, 2012, pp. 42-51.
Toyoizumi T, Ohta R, Kawakami K, et al. Usefulness of combined in vivo skin comet assay and in vivo skin micronucleus test. Mutat Res. 2012;743(1-2):42-51.
Toyoizumi, T., Ohta, R., Kawakami, K., Nakagawa, Y., Tazura, Y., Kuwagata, M., Noguchi, S., Sui, H., & Yamakage, K. (2012). Usefulness of combined in vivo skin comet assay and in vivo skin micronucleus test. Mutation Research, 743(1-2), 42-51. https://doi.org/10.1016/j.mrgentox.2011.12.020
Toyoizumi T, et al. Usefulness of Combined in Vivo Skin Comet Assay and in Vivo Skin Micronucleus Test. Mutat Res. 2012 Mar 18;743(1-2):42-51. PubMed PMID: 22240394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Usefulness of combined in vivo skin comet assay and in vivo skin micronucleus test. AU - Toyoizumi,Tomoyasu, AU - Ohta,Ryo, AU - Kawakami,Kumiko, AU - Nakagawa,Yuzuki, AU - Tazura,Yoshiyuki, AU - Kuwagata,Makiko, AU - Noguchi,Satoshi, AU - Sui,Hajime, AU - Yamakage,Kohji, Y1 - 2012/01/06/ PY - 2011/09/08/received PY - 2011/11/26/revised PY - 2011/12/23/accepted PY - 2012/1/14/entrez PY - 2012/1/14/pubmed PY - 2012/5/23/medline SP - 42 EP - 51 JF - Mutation research JO - Mutat Res VL - 743 IS - 1-2 N2 - We have already found that the in vivo skin comet assay is useful for the evaluation of primary DNA damage induced by genotoxic chemicals in epidermal skin cells. The aim of the present study was to evaluate the sensitivity and specificity of the combined in vivo skin comet assay and in vivo skin micronucleus (MN) test using the same animal to explore the usefulness of the new test method. The combined alkaline comet assay and MN test was carried out with three chemicals: 4-nitroquinoline-1-oxide (4NQO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and benzo[a]pyrene (B[a]P). In the first experiment, we compared DNA- and chromosome-damaging effects of 3 [72, 24 and 3 hours (h) before sacrifice] and 4 applications (72, 48, 24 and 3h before sacrifice) of 4NQO, which induces dermal irritancy. The animals were euthanized and their skin was sampled for the combination test. As a result, the 4-application method was able to detect both DNA- and chromosome-damaging potential with a lower concentration; therefore, in the second experiment, MNNG and B[a]P were topically applied four times, respectively. The animals were euthanized, and then their skins were sampled for combination tests. In the alkaline comet assay, significant differences in the percent of DNA (%DNA) in the tail were observed in epidermal skin cells treated with MNNG and B[a]P. In the MN test, an increased frequency of MN cells (%MN) cells was observed by treatment with MNNG; however, there were no significant increases. In contrast, significant differences in %MN were observed by treatment with B[a]P. From these results, we conclude that the combined in vivo skin comet assay and in vivo MN test was useful because it can detect different genotoxicity with the same sampling time and reduce the number of animals used. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/22240394/Usefulness_of_combined_in_vivo_skin_comet_assay_and_in_vivo_skin_micronucleus_test_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(11)00386-X DB - PRIME DP - Unbound Medicine ER -