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Anthocyanin composition of wild Colombian fruits and antioxidant capacity measurement by electron paramagnetic resonance spectroscopy.
J Agric Food Chem. 2012 Feb 15; 60(6):1397-404.JA

Abstract

The qualitative and quantitative anthocyanin composition of four wild tropical fruits from Colombia was studied. Compounds of "mora pequeña" (Rubus megalococcus Focke.), "uva de árbol" (Myrciaria aff. cauliflora O. Berg), coral, and motilón (Hyeronima macrocarpa Mull. Arg.) fruits were separately extracted with methanol-acetic acid (95:5, v/v). The anthocyanin-rich extracts (AREs) were obtained by selective adsorption on Amberlite XAD-7. Each extract was analyzed by HPLC-PDA and HPLC-HRESI-MS(n) with LCMS-IT-TOF equipment in order to characterize the anthocyanin pigments and the coinjection in HPLC using standards allowed identifying the major constituents in each extract. The antioxidant activity was measured by electron paramagnetic resonance (EPR) and UV-vis spectroscopy, using ABTS and DPPH free radicals. The ARE of motilón (H. macrocarpa Müll. Arg) exhibited the highest radical scavenging activity in comparison to the other extracts. A second-order kinetic model was followed in all of the cases. These results suggested that the studied fruits are promising not only as source of natural pigments but also as antioxidant materials for food industry.

Authors+Show Affiliations

Departamento de Química, Universidad Nacional de Colombia, AA 14490, Bogotá, Colombia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22242913

Citation

Santacruz, Liliana, et al. "Anthocyanin Composition of Wild Colombian Fruits and Antioxidant Capacity Measurement By Electron Paramagnetic Resonance Spectroscopy." Journal of Agricultural and Food Chemistry, vol. 60, no. 6, 2012, pp. 1397-404.
Santacruz L, Carriazo JG, Almanza O, et al. Anthocyanin composition of wild Colombian fruits and antioxidant capacity measurement by electron paramagnetic resonance spectroscopy. J Agric Food Chem. 2012;60(6):1397-404.
Santacruz, L., Carriazo, J. G., Almanza, O., & Osorio, C. (2012). Anthocyanin composition of wild Colombian fruits and antioxidant capacity measurement by electron paramagnetic resonance spectroscopy. Journal of Agricultural and Food Chemistry, 60(6), 1397-404. https://doi.org/10.1021/jf2042533
Santacruz L, et al. Anthocyanin Composition of Wild Colombian Fruits and Antioxidant Capacity Measurement By Electron Paramagnetic Resonance Spectroscopy. J Agric Food Chem. 2012 Feb 15;60(6):1397-404. PubMed PMID: 22242913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anthocyanin composition of wild Colombian fruits and antioxidant capacity measurement by electron paramagnetic resonance spectroscopy. AU - Santacruz,Liliana, AU - Carriazo,José G, AU - Almanza,Ovidio, AU - Osorio,Coralia, Y1 - 2012/02/03/ PY - 2012/1/17/entrez PY - 2012/1/17/pubmed PY - 2012/6/7/medline SP - 1397 EP - 404 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 60 IS - 6 N2 - The qualitative and quantitative anthocyanin composition of four wild tropical fruits from Colombia was studied. Compounds of "mora pequeña" (Rubus megalococcus Focke.), "uva de árbol" (Myrciaria aff. cauliflora O. Berg), coral, and motilón (Hyeronima macrocarpa Mull. Arg.) fruits were separately extracted with methanol-acetic acid (95:5, v/v). The anthocyanin-rich extracts (AREs) were obtained by selective adsorption on Amberlite XAD-7. Each extract was analyzed by HPLC-PDA and HPLC-HRESI-MS(n) with LCMS-IT-TOF equipment in order to characterize the anthocyanin pigments and the coinjection in HPLC using standards allowed identifying the major constituents in each extract. The antioxidant activity was measured by electron paramagnetic resonance (EPR) and UV-vis spectroscopy, using ABTS and DPPH free radicals. The ARE of motilón (H. macrocarpa Müll. Arg) exhibited the highest radical scavenging activity in comparison to the other extracts. A second-order kinetic model was followed in all of the cases. These results suggested that the studied fruits are promising not only as source of natural pigments but also as antioxidant materials for food industry. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22242913/Anthocyanin_composition_of_wild_Colombian_fruits_and_antioxidant_capacity_measurement_by_electron_paramagnetic_resonance_spectroscopy_ L2 - https://doi.org/10.1021/jf2042533 DB - PRIME DP - Unbound Medicine ER -