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Additional role of O-acetylserine as a sulfur status-independent regulator during plant growth.
Plant J 2012; 70(4):666-77PJ

Abstract

O-acetylserine (OAS) is one of the most prominent metabolites whose levels are altered upon sulfur starvation. However, its putative role as a signaling molecule in higher plants is controversial. This paper provides further evidence that OAS is a signaling molecule, based on computational analysis of time-series experiments and on studies of transgenic plants conditionally displaying increased OAS levels. Transcripts whose levels correlated with the transient and specific increase in OAS levels observed in leaves of Arabidopsis thaliana plants 5-10 min after transfer to darkness and with diurnal oscillation of the OAS content, showing a characteristic peak during the night, were identified. Induction of a serine-O-acetyltransferase gene (SERAT) in transgenic A. thaliana plants expressing the genes under the control of an inducible promoter resulted in a specific time-dependent increase in OAS levels. Monitoring the transcriptome response at time points at which no changes in sulfur-related metabolites except OAS were observed and correlating this with the light/dark transition and diurnal experiments resulted in identification of six genes whose expression was highly correlated with that of OAS (adenosine-5'-phosphosulfate reductase 3, sulfur-deficiency-induced 1, sulfur-deficiency-induced 2, low-sulfur-induced 1, serine hydroxymethyltransferase 7 and ChaC-like protein). These data suggest that OAS displays a signalling function leading to changes in transcript levels of a specific gene set irrespective of the sulfur status of the plant. Additionally, a role for OAS in a specific part of the sulfate response can be deduced.

Authors+Show Affiliations

Max Planck Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, Potsdam-Golm, Germany. hubberten@mpimp-golm.mpg.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22243437

Citation

Hubberten, Hans-Michael, et al. "Additional Role of O-acetylserine as a Sulfur Status-independent Regulator During Plant Growth." The Plant Journal : for Cell and Molecular Biology, vol. 70, no. 4, 2012, pp. 666-77.
Hubberten HM, Klie S, Caldana C, et al. Additional role of O-acetylserine as a sulfur status-independent regulator during plant growth. Plant J. 2012;70(4):666-77.
Hubberten, H. M., Klie, S., Caldana, C., Degenkolbe, T., Willmitzer, L., & Hoefgen, R. (2012). Additional role of O-acetylserine as a sulfur status-independent regulator during plant growth. The Plant Journal : for Cell and Molecular Biology, 70(4), pp. 666-77. doi:10.1111/j.1365-313X.2012.04905.x.
Hubberten HM, et al. Additional Role of O-acetylserine as a Sulfur Status-independent Regulator During Plant Growth. Plant J. 2012;70(4):666-77. PubMed PMID: 22243437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Additional role of O-acetylserine as a sulfur status-independent regulator during plant growth. AU - Hubberten,Hans-Michael, AU - Klie,Sebastian, AU - Caldana,Camila, AU - Degenkolbe,Thomas, AU - Willmitzer,Lothar, AU - Hoefgen,Rainer, Y1 - 2012/03/08/ PY - 2012/1/17/entrez PY - 2012/1/17/pubmed PY - 2012/9/11/medline SP - 666 EP - 77 JF - The Plant journal : for cell and molecular biology JO - Plant J. VL - 70 IS - 4 N2 - O-acetylserine (OAS) is one of the most prominent metabolites whose levels are altered upon sulfur starvation. However, its putative role as a signaling molecule in higher plants is controversial. This paper provides further evidence that OAS is a signaling molecule, based on computational analysis of time-series experiments and on studies of transgenic plants conditionally displaying increased OAS levels. Transcripts whose levels correlated with the transient and specific increase in OAS levels observed in leaves of Arabidopsis thaliana plants 5-10 min after transfer to darkness and with diurnal oscillation of the OAS content, showing a characteristic peak during the night, were identified. Induction of a serine-O-acetyltransferase gene (SERAT) in transgenic A. thaliana plants expressing the genes under the control of an inducible promoter resulted in a specific time-dependent increase in OAS levels. Monitoring the transcriptome response at time points at which no changes in sulfur-related metabolites except OAS were observed and correlating this with the light/dark transition and diurnal experiments resulted in identification of six genes whose expression was highly correlated with that of OAS (adenosine-5'-phosphosulfate reductase 3, sulfur-deficiency-induced 1, sulfur-deficiency-induced 2, low-sulfur-induced 1, serine hydroxymethyltransferase 7 and ChaC-like protein). These data suggest that OAS displays a signalling function leading to changes in transcript levels of a specific gene set irrespective of the sulfur status of the plant. Additionally, a role for OAS in a specific part of the sulfate response can be deduced. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/22243437/Additional_role_of_O_acetylserine_as_a_sulfur_status_independent_regulator_during_plant_growth_ L2 - https://doi.org/10.1111/j.1365-313X.2012.04905.x DB - PRIME DP - Unbound Medicine ER -