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Attenuation of colonic inflammation by partial replacement of dietary linoleic acid with α-linolenic acid in a rat model of inflammatory bowel disease.
Br J Nutr. 2012 Nov 14; 108(9):1612-22.BJ

Abstract

Increasing prevalence of inflammatory bowel disease may be due to imbalance in the intake of n-6 and n-3 PUFA in the diet. This study investigates the impact of varying ratios of dietary linoleic acid (LA, 18 : 2n-6) to α-linolenic acid (ALA, 18 : 3n-3) on the inflammatory response in dextran sulphate sodium (DSS)-induced colitis. Weanling male Sprague-Dawley rats were divided into five groups: a non-colitic group with a LA:ALA ratio of 215 (CON-215), and colitic groups with LA:ALA ratios of 215 (DSS-215), 50 (DSS-50), 10 (DSS-10) and 2 (DSS-2). Blends of groundnut, palmolein and linseed oils were used to provide varying LA:ALA ratios. All the rats were fed the respective experimental isoenergetic diets containing 10 % fat for 90 d and DSS was administered during the last 11 d. Colonic inflammation was evaluated by clinical, biochemical and histological parameters. The results showed attenuation of colitis in the DSS-2 group as evidenced by significant reductions in disease activity index, mucosal myeloperoxidase activity (P < 0·05), alkaline phosphatase activity (P < 0·01) and increase in colon length (P < 0·01) compared to the groups fed with higher ratios (DSS-215). This was accompanied by significant reductions in mucosal proinflammatory cytokines TNF-α (P < 0·01) and IL-1β (P < 0·01) and improvement in the histological score. Further, ALA supplementation increased long-chain (LC) n-3 PUFA and decreased LC n-6 PUFA in colon structural lipids. These data suggest that substitution of one-third of LA with ALA (LA:ALA ratio 2) mitigates experimental colitis by down-regulating proinflammatory mediators.

Authors+Show Affiliations

Department of Lipid Chemistry, National Institute of Nutrition, Indian Council of Medical Research, Hyderabad, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22243775

Citation

Tyagi, Anupama, et al. "Attenuation of Colonic Inflammation By Partial Replacement of Dietary Linoleic Acid With Α-linolenic Acid in a Rat Model of Inflammatory Bowel Disease." The British Journal of Nutrition, vol. 108, no. 9, 2012, pp. 1612-22.
Tyagi A, Kumar U, Reddy S, et al. Attenuation of colonic inflammation by partial replacement of dietary linoleic acid with α-linolenic acid in a rat model of inflammatory bowel disease. Br J Nutr. 2012;108(9):1612-22.
Tyagi, A., Kumar, U., Reddy, S., Santosh, V. S., Mohammed, S. B., Ehtesham, N. Z., & Ibrahim, A. (2012). Attenuation of colonic inflammation by partial replacement of dietary linoleic acid with α-linolenic acid in a rat model of inflammatory bowel disease. The British Journal of Nutrition, 108(9), 1612-22. https://doi.org/10.1017/S0007114511007197
Tyagi A, et al. Attenuation of Colonic Inflammation By Partial Replacement of Dietary Linoleic Acid With Α-linolenic Acid in a Rat Model of Inflammatory Bowel Disease. Br J Nutr. 2012 Nov 14;108(9):1612-22. PubMed PMID: 22243775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Attenuation of colonic inflammation by partial replacement of dietary linoleic acid with α-linolenic acid in a rat model of inflammatory bowel disease. AU - Tyagi,Anupama, AU - Kumar,Uday, AU - Reddy,Suryam, AU - Santosh,Vadakattu S, AU - Mohammed,Saazida B, AU - Ehtesham,Nasreen Z, AU - Ibrahim,Ahamed, Y1 - 2012/01/16/ PY - 2012/1/17/entrez PY - 2012/1/17/pubmed PY - 2013/2/13/medline SP - 1612 EP - 22 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 108 IS - 9 N2 - Increasing prevalence of inflammatory bowel disease may be due to imbalance in the intake of n-6 and n-3 PUFA in the diet. This study investigates the impact of varying ratios of dietary linoleic acid (LA, 18 : 2n-6) to α-linolenic acid (ALA, 18 : 3n-3) on the inflammatory response in dextran sulphate sodium (DSS)-induced colitis. Weanling male Sprague-Dawley rats were divided into five groups: a non-colitic group with a LA:ALA ratio of 215 (CON-215), and colitic groups with LA:ALA ratios of 215 (DSS-215), 50 (DSS-50), 10 (DSS-10) and 2 (DSS-2). Blends of groundnut, palmolein and linseed oils were used to provide varying LA:ALA ratios. All the rats were fed the respective experimental isoenergetic diets containing 10 % fat for 90 d and DSS was administered during the last 11 d. Colonic inflammation was evaluated by clinical, biochemical and histological parameters. The results showed attenuation of colitis in the DSS-2 group as evidenced by significant reductions in disease activity index, mucosal myeloperoxidase activity (P < 0·05), alkaline phosphatase activity (P < 0·01) and increase in colon length (P < 0·01) compared to the groups fed with higher ratios (DSS-215). This was accompanied by significant reductions in mucosal proinflammatory cytokines TNF-α (P < 0·01) and IL-1β (P < 0·01) and improvement in the histological score. Further, ALA supplementation increased long-chain (LC) n-3 PUFA and decreased LC n-6 PUFA in colon structural lipids. These data suggest that substitution of one-third of LA with ALA (LA:ALA ratio 2) mitigates experimental colitis by down-regulating proinflammatory mediators. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/22243775/Attenuation_of_colonic_inflammation_by_partial_replacement_of_dietary_linoleic_acid_with_α_linolenic_acid_in_a_rat_model_of_inflammatory_bowel_disease_ L2 - https://www.cambridge.org/core/product/identifier/S0007114511007197/type/journal_article DB - PRIME DP - Unbound Medicine ER -