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Electrochemiluminescence biosensor for the assay of small molecule and protein based on bifunctional aptamer and chemiluminescent functionalized gold nanoparticles.
Anal Chim Acta. 2012 Feb 17; 715:86-92.AC

Abstract

An electrochemiluminescence (ECL) biosensor for simultaneous detection of adenosine and thrombin in one sample based on bifunctional aptamer and N-(aminobutyl)-N-(ethylisoluminol) functionalized gold nanoparticles (ABEI-AuNPs) was developed. A streptavidin coated gold nanoparticles modified electrode was utilized to immobilize biotinylated bifunctional aptamer (ATA), which consisted of adenosine and thrombin aptamer. The ATA performed as recognition element of capture probe. For adenosine detection, ABEI-AuNPs labeled hybridization probe with a partial complementary sequence of ATA reacted with ATA, leading to a strong ECL response of N-(aminobutyl)-N-(ethylisoluminol) enriched on ABEI-AuNPs. After recognition of adenosine, the hybridization probe was displaced by adenosine and ECL signal declined. The decrease of ECL signal was in proportion to the concentration of adenosine over the range of 5.0×10(-12)-5.0×10(-9) M with a detection limit of 2.2×10(-12) M. For thrombin detection, thrombin was assembled on ATA modified electrode via aptamer-target recognition, another aptamer of thrombin tagged with ABEI-AuNPs was bounded to another reactive site of thrombin, producing ECL signals. The ECL intensity was linearly with the concentration of thrombin from 5×10(-14)M to 5×10(-10) M with a detection limit of 1.2×10(-14) M. In the ECL biosensor, adenosine and thrombin can be detected when they coexisted in one sample and a multi-analytes assay was established. The sensitivity of the present biosensor is superior to most available aptasensors for adenosine and thrombin. The biosensor also showed good selectivity towards the targets. Being challenged in real plasma sample, the biosensor was confirmed to be a good prospect for multi-analytes assay of small molecules and proteins in biological samples.

Authors+Show Affiliations

CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22244171

Citation

Chai, Ying, et al. "Electrochemiluminescence Biosensor for the Assay of Small Molecule and Protein Based On Bifunctional Aptamer and Chemiluminescent Functionalized Gold Nanoparticles." Analytica Chimica Acta, vol. 715, 2012, pp. 86-92.
Chai Y, Tian D, Cui H. Electrochemiluminescence biosensor for the assay of small molecule and protein based on bifunctional aptamer and chemiluminescent functionalized gold nanoparticles. Anal Chim Acta. 2012;715:86-92.
Chai, Y., Tian, D., & Cui, H. (2012). Electrochemiluminescence biosensor for the assay of small molecule and protein based on bifunctional aptamer and chemiluminescent functionalized gold nanoparticles. Analytica Chimica Acta, 715, 86-92. https://doi.org/10.1016/j.aca.2011.12.006
Chai Y, Tian D, Cui H. Electrochemiluminescence Biosensor for the Assay of Small Molecule and Protein Based On Bifunctional Aptamer and Chemiluminescent Functionalized Gold Nanoparticles. Anal Chim Acta. 2012 Feb 17;715:86-92. PubMed PMID: 22244171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemiluminescence biosensor for the assay of small molecule and protein based on bifunctional aptamer and chemiluminescent functionalized gold nanoparticles. AU - Chai,Ying, AU - Tian,Dayong, AU - Cui,Hua, Y1 - 2011/12/14/ PY - 2011/09/09/received PY - 2011/11/20/revised PY - 2011/12/05/accepted PY - 2012/1/17/entrez PY - 2012/1/17/pubmed PY - 2012/5/4/medline SP - 86 EP - 92 JF - Analytica chimica acta JO - Anal Chim Acta VL - 715 N2 - An electrochemiluminescence (ECL) biosensor for simultaneous detection of adenosine and thrombin in one sample based on bifunctional aptamer and N-(aminobutyl)-N-(ethylisoluminol) functionalized gold nanoparticles (ABEI-AuNPs) was developed. A streptavidin coated gold nanoparticles modified electrode was utilized to immobilize biotinylated bifunctional aptamer (ATA), which consisted of adenosine and thrombin aptamer. The ATA performed as recognition element of capture probe. For adenosine detection, ABEI-AuNPs labeled hybridization probe with a partial complementary sequence of ATA reacted with ATA, leading to a strong ECL response of N-(aminobutyl)-N-(ethylisoluminol) enriched on ABEI-AuNPs. After recognition of adenosine, the hybridization probe was displaced by adenosine and ECL signal declined. The decrease of ECL signal was in proportion to the concentration of adenosine over the range of 5.0×10(-12)-5.0×10(-9) M with a detection limit of 2.2×10(-12) M. For thrombin detection, thrombin was assembled on ATA modified electrode via aptamer-target recognition, another aptamer of thrombin tagged with ABEI-AuNPs was bounded to another reactive site of thrombin, producing ECL signals. The ECL intensity was linearly with the concentration of thrombin from 5×10(-14)M to 5×10(-10) M with a detection limit of 1.2×10(-14) M. In the ECL biosensor, adenosine and thrombin can be detected when they coexisted in one sample and a multi-analytes assay was established. The sensitivity of the present biosensor is superior to most available aptasensors for adenosine and thrombin. The biosensor also showed good selectivity towards the targets. Being challenged in real plasma sample, the biosensor was confirmed to be a good prospect for multi-analytes assay of small molecules and proteins in biological samples. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/22244171/Electrochemiluminescence_biosensor_for_the_assay_of_small_molecule_and_protein_based_on_bifunctional_aptamer_and_chemiluminescent_functionalized_gold_nanoparticles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(11)01671-0 DB - PRIME DP - Unbound Medicine ER -