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In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces.

Abstract

Various chemical scolicidal agents have been used for inactivation of hydatid cyst protoscolices, but most of them are associated with adverse side effects. Since ajowan (Trachyspermum ammi) has been shown to have a number of medicinal properties, in this study the scolicidal effect of the essential oil (EO) from the fruits of this herbal plant was investigated. Ajowan EO was obtained by hydrodistillation method. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to determine the chemical composition of the EO. Protoscoleces were exposed to various concentrations of EO (3, 5 and 10mg/mL) for 10, 20, 30, and 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. A total of 18 compounds representing 99.54% of the total oil, were identified. Thymol (50.07%), γ-terpinene (23.92%), and p-cymene (22.9%) were found to be the major EO constituents. While the mortality rate of protoscolices in the control group was 6.67%, scolicidal power of ajowan EO at concentration of 3mg/mL was 31.34, 35.98, 45.17, and 51.58% after 10, 20, 30, and 60 min, respectively. The EO at concentration of 5mg/mL killed 51.89, 72.20, 88.64, and 100% of protoscolices after 10, 20, 30, and 60 min, respectively. One hundred percent scolicidal activity was observed with ajowan EO at concentration of 10mg/mL after 10 min of exposure. The results of this study revealed that the EO of ajowan is rich in thymol, γ-terpinene and p-cymene, has high scolicidal power and it may be used as a natural scolicidal agent.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    School of Veterinary Medicine, Shiraz University, Shiraz 71345-1731, Iran. moazeni@shirazu.ac.ir

    ,

    Source

    Veterinary parasitology 187:1-2 2012 Jun 08 pg 203-8

    MeSH

    Animals
    Anthelmintics
    Carum
    Echinococcus granulosus
    Oils, Volatile
    Plant Oils

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22245070

    Citation

    Moazeni, Mohammad, et al. "In Vitro Lethal Effect of Ajowan (Trachyspermum Ammi L.) Essential Oil On Hydatid Cyst Protoscoleces." Veterinary Parasitology, vol. 187, no. 1-2, 2012, pp. 203-8.
    Moazeni M, Saharkhiz MJ, Hosseini AA. In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces. Vet Parasitol. 2012;187(1-2):203-8.
    Moazeni, M., Saharkhiz, M. J., & Hosseini, A. A. (2012). In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces. Veterinary Parasitology, 187(1-2), pp. 203-8. doi:10.1016/j.vetpar.2011.12.025.
    Moazeni M, Saharkhiz MJ, Hosseini AA. In Vitro Lethal Effect of Ajowan (Trachyspermum Ammi L.) Essential Oil On Hydatid Cyst Protoscoleces. Vet Parasitol. 2012 Jun 8;187(1-2):203-8. PubMed PMID: 22245070.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vitro lethal effect of ajowan (Trachyspermum ammi L.) essential oil on hydatid cyst protoscoleces. AU - Moazeni,Mohammad, AU - Saharkhiz,Mohammad Jamal, AU - Hosseini,Ali Akbar, Y1 - 2011/12/28/ PY - 2011/07/29/received PY - 2011/12/15/revised PY - 2011/12/20/accepted PY - 2012/1/17/entrez PY - 2012/1/17/pubmed PY - 2012/10/2/medline SP - 203 EP - 8 JF - Veterinary parasitology JO - Vet. Parasitol. VL - 187 IS - 1-2 N2 - Various chemical scolicidal agents have been used for inactivation of hydatid cyst protoscolices, but most of them are associated with adverse side effects. Since ajowan (Trachyspermum ammi) has been shown to have a number of medicinal properties, in this study the scolicidal effect of the essential oil (EO) from the fruits of this herbal plant was investigated. Ajowan EO was obtained by hydrodistillation method. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to determine the chemical composition of the EO. Protoscoleces were exposed to various concentrations of EO (3, 5 and 10mg/mL) for 10, 20, 30, and 60 min. Viability of protoscolices was confirmed by 0.1% eosin staining. A total of 18 compounds representing 99.54% of the total oil, were identified. Thymol (50.07%), γ-terpinene (23.92%), and p-cymene (22.9%) were found to be the major EO constituents. While the mortality rate of protoscolices in the control group was 6.67%, scolicidal power of ajowan EO at concentration of 3mg/mL was 31.34, 35.98, 45.17, and 51.58% after 10, 20, 30, and 60 min, respectively. The EO at concentration of 5mg/mL killed 51.89, 72.20, 88.64, and 100% of protoscolices after 10, 20, 30, and 60 min, respectively. One hundred percent scolicidal activity was observed with ajowan EO at concentration of 10mg/mL after 10 min of exposure. The results of this study revealed that the EO of ajowan is rich in thymol, γ-terpinene and p-cymene, has high scolicidal power and it may be used as a natural scolicidal agent. SN - 1873-2550 UR - https://www.unboundmedicine.com/medline/citation/22245070/In_vitro_lethal_effect_of_ajowan__Trachyspermum_ammi_L___essential_oil_on_hydatid_cyst_protoscoleces_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(11)00863-6 DB - PRIME DP - Unbound Medicine ER -