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Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay.
J Med Virol. 2012 Mar; 84(3):490-9.JM

Abstract

Dengue virus (DV) infection is the most common mosquito-born viral disease of public health significance. Though most patients only suffer from flu-like symptoms, a small group of patients experiences more severe forms of the disease. The viral nonstructural protein 1 (NS1), a secreted protein correlating with viremia, is a key element used for dengue diagnosis with potential implications in severe dengue prognosis. Capture-ELISAs for the early detection of the NS1 protein in the sera during the acute febrile stage are commonly used in routine by diagnostic laboratories. In this study, the detection of NS1 protein in DV-infected material was assessed by an alternative method combining a single NS1-directed monoclonal antibody and the SELDI-TOF/MS technology. According to the epitope mapping, the antibodies used are mainly directed against an immuno-dominant peptide located on the C-terminal part of the protein. The NS1 SELDI-TOF assay is specific, has a sensitivity level close to capture-ELISAs and is potentially useful for a coupled serotyping/detection assay or for the detection of subtle post-translational modifications on the protein.

Authors+Show Affiliations

Biomarker Department, BioMerieux SA, Chemin de l'Orme, Marcy l'Etoile, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22246837

Citation

Steidel, Marine, et al. "Nonstructural Protein NS1 Immunodominant Epitope Detected Specifically in Dengue Virus Infected Material By a SELDI-TOF/MS Based Assay." Journal of Medical Virology, vol. 84, no. 3, 2012, pp. 490-9.
Steidel M, Fragnoud R, Guillotte M, et al. Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay. J Med Virol. 2012;84(3):490-9.
Steidel, M., Fragnoud, R., Guillotte, M., Roesch, C., Michel, S., Meunier, T., Paranhos-Baccalà, G., Gervasi, G., & Bedin, F. (2012). Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay. Journal of Medical Virology, 84(3), 490-9. https://doi.org/10.1002/jmv.23204
Steidel M, et al. Nonstructural Protein NS1 Immunodominant Epitope Detected Specifically in Dengue Virus Infected Material By a SELDI-TOF/MS Based Assay. J Med Virol. 2012;84(3):490-9. PubMed PMID: 22246837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nonstructural protein NS1 immunodominant epitope detected specifically in dengue virus infected material by a SELDI-TOF/MS based assay. AU - Steidel,Marine, AU - Fragnoud,Romain, AU - Guillotte,Michelle, AU - Roesch,Céline, AU - Michel,Sandrine, AU - Meunier,Thomas, AU - Paranhos-Baccalà,Glàucia, AU - Gervasi,Gaspard, AU - Bedin,Frederic, PY - 2012/1/17/entrez PY - 2012/1/17/pubmed PY - 2012/6/14/medline SP - 490 EP - 9 JF - Journal of medical virology JO - J Med Virol VL - 84 IS - 3 N2 - Dengue virus (DV) infection is the most common mosquito-born viral disease of public health significance. Though most patients only suffer from flu-like symptoms, a small group of patients experiences more severe forms of the disease. The viral nonstructural protein 1 (NS1), a secreted protein correlating with viremia, is a key element used for dengue diagnosis with potential implications in severe dengue prognosis. Capture-ELISAs for the early detection of the NS1 protein in the sera during the acute febrile stage are commonly used in routine by diagnostic laboratories. In this study, the detection of NS1 protein in DV-infected material was assessed by an alternative method combining a single NS1-directed monoclonal antibody and the SELDI-TOF/MS technology. According to the epitope mapping, the antibodies used are mainly directed against an immuno-dominant peptide located on the C-terminal part of the protein. The NS1 SELDI-TOF assay is specific, has a sensitivity level close to capture-ELISAs and is potentially useful for a coupled serotyping/detection assay or for the detection of subtle post-translational modifications on the protein. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/22246837/Nonstructural_protein_NS1_immunodominant_epitope_detected_specifically_in_dengue_virus_infected_material_by_a_SELDI_TOF/MS_based_assay_ L2 - https://doi.org/10.1002/jmv.23204 DB - PRIME DP - Unbound Medicine ER -