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Effects of supplemental erythropoietin on its receptor expression and signal transduction pathways in rat model of retinal detachment.
Curr Eye Res. 2012 Feb; 37(2):138-44.CE

Abstract

PURPOSE

The aim of this study was to investigate the effects of supplemental erythropoietin (EPO) on its receptor (EPOR) and signal transduction pathways in rat model of retinal detachment (RD).

METHODS

To investigate the effect of EPO on EPOR expression in RD rats 100, 200 or 400 ng EPO was injected into the vitreous cavity immediately after RD model was induced. Western blot and immunohistochemistry analyses were performed to measure EPOR expression. To investigate the effect of EPO on signal transduction pathways in RD rats single dose of 400 ng EPO was injected into the vitreous cavity immediately after RD model was induced. The total and phosphorylated levels of JAK2, Akt, ERK-1/2, STAT5 and NF-κB were assessed by western blot.

RESULTS

Western blot analysis showed that, compared with the normal control group, EPOR expression in the neurosensory retina was significantly increased in experimental RD groups (P < 0.05), but the differences were not significant between experimental RD groups (P > 0.05). Immunohistochemical examination indicated that EPOR staining on retinas became strongly positive 3 days after RD, with no significant difference in staining intensities between the treatment groups. Phosphorylated levels of JAK2, Akt, ERK-1/2, STAT5, and NF-κB were enhanced 3 days after RD, but only JAK2, Akt, and ERK-1/2 phosphorylation was further enhanced by 400 ng EPO treatment (P < 0.05).

CONCLUSIONS

Supplementary EPO cannot affect EPOR expression in detached retina, but EPO may activate both PI-3K/Akt and MAPK/ERK-1/2 signal transduction pathways in RD model.

Authors+Show Affiliations

Department of Ophthalmology, Clinical Medicine School, Yangzhou University, Subei People's Hospital of Jiangsu Province, Yangzhou, China. zgxie87@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22251399

Citation

Xie, Zhenggao, et al. "Effects of Supplemental Erythropoietin On Its Receptor Expression and Signal Transduction Pathways in Rat Model of Retinal Detachment." Current Eye Research, vol. 37, no. 2, 2012, pp. 138-44.
Xie Z, Chen F, Wu X, et al. Effects of supplemental erythropoietin on its receptor expression and signal transduction pathways in rat model of retinal detachment. Curr Eye Res. 2012;37(2):138-44.
Xie, Z., Chen, F., Wu, X., Zhuang, C., Zhu, J., Wang, J., Ji, H., Wang, Y., & Hua, X. (2012). Effects of supplemental erythropoietin on its receptor expression and signal transduction pathways in rat model of retinal detachment. Current Eye Research, 37(2), 138-44. https://doi.org/10.3109/02713683.2011.647225
Xie Z, et al. Effects of Supplemental Erythropoietin On Its Receptor Expression and Signal Transduction Pathways in Rat Model of Retinal Detachment. Curr Eye Res. 2012;37(2):138-44. PubMed PMID: 22251399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of supplemental erythropoietin on its receptor expression and signal transduction pathways in rat model of retinal detachment. AU - Xie,Zhenggao, AU - Chen,Fang, AU - Wu,Xingwei, AU - Zhuang,Chaorong, AU - Zhu,Jun, AU - Wang,Jian, AU - Ji,Heqing, AU - Wang,Yakun, AU - Hua,Xin, PY - 2012/1/19/entrez PY - 2012/1/19/pubmed PY - 2012/5/24/medline SP - 138 EP - 44 JF - Current eye research JO - Curr Eye Res VL - 37 IS - 2 N2 - PURPOSE: The aim of this study was to investigate the effects of supplemental erythropoietin (EPO) on its receptor (EPOR) and signal transduction pathways in rat model of retinal detachment (RD). METHODS: To investigate the effect of EPO on EPOR expression in RD rats 100, 200 or 400 ng EPO was injected into the vitreous cavity immediately after RD model was induced. Western blot and immunohistochemistry analyses were performed to measure EPOR expression. To investigate the effect of EPO on signal transduction pathways in RD rats single dose of 400 ng EPO was injected into the vitreous cavity immediately after RD model was induced. The total and phosphorylated levels of JAK2, Akt, ERK-1/2, STAT5 and NF-κB were assessed by western blot. RESULTS: Western blot analysis showed that, compared with the normal control group, EPOR expression in the neurosensory retina was significantly increased in experimental RD groups (P < 0.05), but the differences were not significant between experimental RD groups (P > 0.05). Immunohistochemical examination indicated that EPOR staining on retinas became strongly positive 3 days after RD, with no significant difference in staining intensities between the treatment groups. Phosphorylated levels of JAK2, Akt, ERK-1/2, STAT5, and NF-κB were enhanced 3 days after RD, but only JAK2, Akt, and ERK-1/2 phosphorylation was further enhanced by 400 ng EPO treatment (P < 0.05). CONCLUSIONS: Supplementary EPO cannot affect EPOR expression in detached retina, but EPO may activate both PI-3K/Akt and MAPK/ERK-1/2 signal transduction pathways in RD model. SN - 1460-2202 UR - https://www.unboundmedicine.com/medline/citation/22251399/Effects_of_supplemental_erythropoietin_on_its_receptor_expression_and_signal_transduction_pathways_in_rat_model_of_retinal_detachment_ L2 - https://www.tandfonline.com/doi/full/10.3109/02713683.2011.647225 DB - PRIME DP - Unbound Medicine ER -