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Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, lacZα gene and kanamycin or hygromycin resistance marker.
Plasmid 2012; 67(3):272-5P

Abstract

Two versatile shuttle vectors for Thermus thermophilus and Escherichia coli were developed on the basis of the T. thermophilus cryptic plasmid pTT8 and E. coli vector pUC13. These shuttle vectors, pTRK1T and pTRH1T, carry a gene encoding a protein homologous to replication protein derived from pTT8, a replicon for E. coli, new multiple cloning sites and a lacZα gene from E. coli vector pUC13, and also have a gene encoding a thermostable protein that confers resistance to kanamycin or hygromycin, which can be used as a selection marker in T. thermophilus. These shuttle vectors are useful to develop enzymes and proteins of biotechnological interest. We also constructed a plasmid, pUC13T, which carries the same multiple cloning sites of pTRK1T and pTRH1T. These vectors should facilitate cloning procedures both in E. coli and T. thermophilus.

Authors+Show Affiliations

Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan. a-fujita@aist.go.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22252135

Citation

Fujita, Atsushi, et al. "Two Versatile Shuttle Vectors for Thermus thermophilus-Escherichia Coli Containing Multiple Cloning Sites, lacZα Gene and Kanamycin or Hygromycin Resistance Marker." Plasmid, vol. 67, no. 3, 2012, pp. 272-5.
Fujita A, Misumi Y, Koyama Y. Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, lacZα gene and kanamycin or hygromycin resistance marker. Plasmid. 2012;67(3):272-5.
Fujita, A., Misumi, Y., & Koyama, Y. (2012). Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, lacZα gene and kanamycin or hygromycin resistance marker. Plasmid, 67(3), pp. 272-5. doi:10.1016/j.plasmid.2011.12.013.
Fujita A, Misumi Y, Koyama Y. Two Versatile Shuttle Vectors for Thermus thermophilus-Escherichia Coli Containing Multiple Cloning Sites, lacZα Gene and Kanamycin or Hygromycin Resistance Marker. Plasmid. 2012;67(3):272-5. PubMed PMID: 22252135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, lacZα gene and kanamycin or hygromycin resistance marker. AU - Fujita,Atsushi, AU - Misumi,Yoshio, AU - Koyama,Yoshinori, Y1 - 2012/01/10/ PY - 2011/10/25/received PY - 2011/12/27/revised PY - 2011/12/28/accepted PY - 2012/1/19/entrez PY - 2012/1/19/pubmed PY - 2012/9/11/medline SP - 272 EP - 5 JF - Plasmid JO - Plasmid VL - 67 IS - 3 N2 - Two versatile shuttle vectors for Thermus thermophilus and Escherichia coli were developed on the basis of the T. thermophilus cryptic plasmid pTT8 and E. coli vector pUC13. These shuttle vectors, pTRK1T and pTRH1T, carry a gene encoding a protein homologous to replication protein derived from pTT8, a replicon for E. coli, new multiple cloning sites and a lacZα gene from E. coli vector pUC13, and also have a gene encoding a thermostable protein that confers resistance to kanamycin or hygromycin, which can be used as a selection marker in T. thermophilus. These shuttle vectors are useful to develop enzymes and proteins of biotechnological interest. We also constructed a plasmid, pUC13T, which carries the same multiple cloning sites of pTRK1T and pTRH1T. These vectors should facilitate cloning procedures both in E. coli and T. thermophilus. SN - 1095-9890 UR - https://www.unboundmedicine.com/medline/citation/22252135/Two_versatile_shuttle_vectors_for_Thermus_thermophilus_Escherichia_coli_containing_multiple_cloning_sites_lacZα_gene_and_kanamycin_or_hygromycin_resistance_marker_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-619X(11)00121-1 DB - PRIME DP - Unbound Medicine ER -