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Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin, and impact on antimicrobial properties and toxicity against Artemia salina leach.
Chem Biodivers. 2012 Jan; 9(1):139-50.CB

Abstract

To determine the period of harvest that optimizes the antimicrobial activities of the essential oil of Ocimum gratissimum L. from Benin, aerial plant parts were collected at two vegetative stages (pre- and full-flowering) and three sampling times (7 am, 1 pm, and 7 pm). Extraction by hydrodistillation yielded between 0.65 and 0.78% of essential oils. Characterization of the oils by GC-FID and GC/MS analysis revealed the presence of monoterpenes (87.26-93.81%), sesquiterpenes (5.57-11.34%), and aliphatic compounds (0.15-0.18%), with p-cymene (1; 28.08-53.82%), thymol (2; 3.32-29.13%), γ-terpinene (3; 1.11-10.91%), α-thujene (4; 3.37-10.77%), and β-myrcene (5; 4.24-8.28%) as major components. Two chemotypes were observed, i.e., a p-cymene/thymol and a p-cymene chemotype, for plants harvested at 7 am for the former and at 1 pm or 7 pm for the latter, respectively. The oils were fungicidal against Candida albicans, with the sample from full-flowering plants collected at 7 am being the most active (MIC = 0.06±0.00 mg/ml). The chemical variation of the oils also influenced the antimicrobial effect against Staphylococcus aureus; the most active oil was obtained from plants at the pre-flowering stage collected at 7 am (MIC=0.24±0.01 mg/ml). Escherichia coli was insensitive to the chemical variation of the oils (MICs of ca. 0.48±0.02 mg/ml for all oils). Moreover, the essential oils showed low toxicity against Artemia salina Leach larvae, with LC(50) values in the range of 43-146 μg/ml. This is the first study of the interaction between the daytime of collection and vegetative stage of the plants and the antimicrobial properties and toxicity of the essential oil of O. gratissimum from Benin.

Authors+Show Affiliations

Laboratory of Physic and Synthesis Organic Chemistry (LaCOPS), University of Abomey-Calavi, Faculty of Sciences and Technics, BP: 4521 Cotonou, Benin.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22253111

Citation

Kpadonou Kpoviessi, Bénédicta G H., et al. "Chemical Variation of Essential Oil Constituents of Ocimum Gratissimum L. From Benin, and Impact On Antimicrobial Properties and Toxicity Against Artemia Salina Leach." Chemistry & Biodiversity, vol. 9, no. 1, 2012, pp. 139-50.
Kpadonou Kpoviessi BG, Ladekan EY, Kpoviessi DS, et al. Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin, and impact on antimicrobial properties and toxicity against Artemia salina leach. Chem Biodivers. 2012;9(1):139-50.
Kpadonou Kpoviessi, B. G., Ladekan, E. Y., Kpoviessi, D. S., Gbaguidi, F., Yehouenou, B., Quetin-Leclercq, J., Figueredo, G., Moudachirou, M., & Accrombessi, G. C. (2012). Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin, and impact on antimicrobial properties and toxicity against Artemia salina leach. Chemistry & Biodiversity, 9(1), 139-50. https://doi.org/10.1002/cbdv.201100194
Kpadonou Kpoviessi BG, et al. Chemical Variation of Essential Oil Constituents of Ocimum Gratissimum L. From Benin, and Impact On Antimicrobial Properties and Toxicity Against Artemia Salina Leach. Chem Biodivers. 2012;9(1):139-50. PubMed PMID: 22253111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical variation of essential oil constituents of Ocimum gratissimum L. from Benin, and impact on antimicrobial properties and toxicity against Artemia salina leach. AU - Kpadonou Kpoviessi,Bénédicta G H, AU - Ladekan,Eléonore Yayi, AU - Kpoviessi,D S Salomé, AU - Gbaguidi,Fernand, AU - Yehouenou,Boniface, AU - Quetin-Leclercq,Joëlle, AU - Figueredo,Gilles, AU - Moudachirou,Mansourou, AU - Accrombessi,Georges C, PY - 2012/1/19/entrez PY - 2012/1/19/pubmed PY - 2012/5/15/medline SP - 139 EP - 50 JF - Chemistry & biodiversity JO - Chem Biodivers VL - 9 IS - 1 N2 - To determine the period of harvest that optimizes the antimicrobial activities of the essential oil of Ocimum gratissimum L. from Benin, aerial plant parts were collected at two vegetative stages (pre- and full-flowering) and three sampling times (7 am, 1 pm, and 7 pm). Extraction by hydrodistillation yielded between 0.65 and 0.78% of essential oils. Characterization of the oils by GC-FID and GC/MS analysis revealed the presence of monoterpenes (87.26-93.81%), sesquiterpenes (5.57-11.34%), and aliphatic compounds (0.15-0.18%), with p-cymene (1; 28.08-53.82%), thymol (2; 3.32-29.13%), γ-terpinene (3; 1.11-10.91%), α-thujene (4; 3.37-10.77%), and β-myrcene (5; 4.24-8.28%) as major components. Two chemotypes were observed, i.e., a p-cymene/thymol and a p-cymene chemotype, for plants harvested at 7 am for the former and at 1 pm or 7 pm for the latter, respectively. The oils were fungicidal against Candida albicans, with the sample from full-flowering plants collected at 7 am being the most active (MIC = 0.06±0.00 mg/ml). The chemical variation of the oils also influenced the antimicrobial effect against Staphylococcus aureus; the most active oil was obtained from plants at the pre-flowering stage collected at 7 am (MIC=0.24±0.01 mg/ml). Escherichia coli was insensitive to the chemical variation of the oils (MICs of ca. 0.48±0.02 mg/ml for all oils). Moreover, the essential oils showed low toxicity against Artemia salina Leach larvae, with LC(50) values in the range of 43-146 μg/ml. This is the first study of the interaction between the daytime of collection and vegetative stage of the plants and the antimicrobial properties and toxicity of the essential oil of O. gratissimum from Benin. SN - 1612-1880 UR - https://www.unboundmedicine.com/medline/citation/22253111/Chemical_variation_of_essential_oil_constituents_of_Ocimum_gratissimum_L__from_Benin_and_impact_on_antimicrobial_properties_and_toxicity_against_Artemia_salina_leach_ L2 - https://doi.org/10.1002/cbdv.201100194 DB - PRIME DP - Unbound Medicine ER -