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Neuronal nitric oxide synthase, nNOS, regulates renal hemodynamics in the postnatal developing piglet.
Pediatr Res. 2012 Feb; 71(2):144-9.PR

Abstract

INTRODUCTION

Nitric oxide (NO) vasodilation critically modulates renal hemodynamics in the neonate compared with the adult. Based on the postnatal expression pattern of renal neuronal nitric oxide synthase (nNOS), the hypothesis was that nNOS is the major NOS isoform regulating renal hemodynamics in the immature, but not mature, kidney.

RESULTS

NOS inhibitors did not alter mean arterial pressure (MAP) in either group. Intrarenal S-methyl-L-thiocitrulline (L-SMTC) in newborns significantly reduced renal blood flow (RBF) 38 ± 4%, glomerular filtration rate (GFR) 42 ± 6%, and increased renal vascular resistance (RVR) 37 ± 7%, whereas intrarenal L-nitro-arginine methyl ester (L-NAME) affected RBF, GFR, and RVR equivalent to L-SMTC treatment. When L-NAME was administered after L-SMTC treatment, newborn renal hemodynamic changes were not further altered from what was observed when L-SMTC was administered alone. In contrast, in the adult, only intrarenal L-NAME, and not L-SMTC, affected renal hemodynamic responses.

DISCUSSION

In conclusion, these studies demonstrate that nNOS is an important regulator of renal hemodynamics in the newborn kidney, but not in the adult.

METHODS

Experiments compared renal hemodynamic responses with intrarenal infusion of L-NAME, an inhibitor of all NOS isoforms, with the selective nNOS inhibitor L-SMTC in the newborn piglet and the adult pig.

Authors+Show Affiliations

Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

22258124

Citation

Rodebaugh, Justin, et al. "Neuronal Nitric Oxide Synthase, nNOS, Regulates Renal Hemodynamics in the Postnatal Developing Piglet." Pediatric Research, vol. 71, no. 2, 2012, pp. 144-9.
Rodebaugh J, Sekulic M, Davies W, et al. Neuronal nitric oxide synthase, nNOS, regulates renal hemodynamics in the postnatal developing piglet. Pediatr Res. 2012;71(2):144-9.
Rodebaugh, J., Sekulic, M., Davies, W., Montgomery, S., Khraibi, A., Solhaug, M. J., & Ratliff, B. B. (2012). Neuronal nitric oxide synthase, nNOS, regulates renal hemodynamics in the postnatal developing piglet. Pediatric Research, 71(2), 144-9. https://doi.org/10.1038/pr.2011.23
Rodebaugh J, et al. Neuronal Nitric Oxide Synthase, nNOS, Regulates Renal Hemodynamics in the Postnatal Developing Piglet. Pediatr Res. 2012;71(2):144-9. PubMed PMID: 22258124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuronal nitric oxide synthase, nNOS, regulates renal hemodynamics in the postnatal developing piglet. AU - Rodebaugh,Justin, AU - Sekulic,Miroslav, AU - Davies,William, AU - Montgomery,Stephanie, AU - Khraibi,Ali, AU - Solhaug,Michael J, AU - Ratliff,Brian B, Y1 - 2011/12/21/ PY - 2012/1/20/entrez PY - 2012/1/20/pubmed PY - 2012/6/7/medline SP - 144 EP - 9 JF - Pediatric research JO - Pediatr Res VL - 71 IS - 2 N2 - INTRODUCTION: Nitric oxide (NO) vasodilation critically modulates renal hemodynamics in the neonate compared with the adult. Based on the postnatal expression pattern of renal neuronal nitric oxide synthase (nNOS), the hypothesis was that nNOS is the major NOS isoform regulating renal hemodynamics in the immature, but not mature, kidney. RESULTS: NOS inhibitors did not alter mean arterial pressure (MAP) in either group. Intrarenal S-methyl-L-thiocitrulline (L-SMTC) in newborns significantly reduced renal blood flow (RBF) 38 ± 4%, glomerular filtration rate (GFR) 42 ± 6%, and increased renal vascular resistance (RVR) 37 ± 7%, whereas intrarenal L-nitro-arginine methyl ester (L-NAME) affected RBF, GFR, and RVR equivalent to L-SMTC treatment. When L-NAME was administered after L-SMTC treatment, newborn renal hemodynamic changes were not further altered from what was observed when L-SMTC was administered alone. In contrast, in the adult, only intrarenal L-NAME, and not L-SMTC, affected renal hemodynamic responses. DISCUSSION: In conclusion, these studies demonstrate that nNOS is an important regulator of renal hemodynamics in the newborn kidney, but not in the adult. METHODS: Experiments compared renal hemodynamic responses with intrarenal infusion of L-NAME, an inhibitor of all NOS isoforms, with the selective nNOS inhibitor L-SMTC in the newborn piglet and the adult pig. SN - 1530-0447 UR - https://www.unboundmedicine.com/medline/citation/22258124/Neuronal_nitric_oxide_synthase_nNOS_regulates_renal_hemodynamics_in_the_postnatal_developing_piglet_ L2 - https://doi.org/10.1038/pr.2011.23 DB - PRIME DP - Unbound Medicine ER -