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Antioxidant, antimicrobial, and anticancer activity of 3 Umbilicaria species.
J Food Sci. 2012 Jan; 77(1):T20-5.JF

Abstract

The aim of this study is to investigate in vitro antioxidant, antimicrobial, and anticancer activity of the acetone extracts of the lichens Umbilicaria crustulosa, U. cylindrica, and U. polyphylla. Antioxidant activity was evaluated by 5 separate methods: free radical scavenging, superoxide anion radical scavenging, reducing power, determination of total phenolic compounds, and determination of total flavonoid content. Of the lichens tested, U. polyphylla had largest free radical scavenging activity (72.79% inhibition at a concentration of 1 mg/mL), which was similar as standard antioxidants in the same concentration. Moreover, the tested extracts had effective reducing power and superoxide anion radical scavenging. Total content of phenol and flavonoid in extracts was determined as pyrocatechol equivalent, and as rutin equivalent, respectively. The strong relationships between total phenolic and flavonoid contents and the antioxidant effect of tested extracts were observed. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method. The most active was extract of U. polyphylla with minimum inhibitory concentration values ranging from 1.56 to 12.5 mg/mL. Anticancer activity was tested against FemX (human melanoma) and LS174 (human colon carcinoma) cell lines using MTT method. All extracts were found to be strong anticancer activity toward both cell lines with IC₅₀ values ranging from 28.45 to 97.82 μg/mL. The present study shows that tested lichen extracts demonstrated a strong antioxidant, antimicrobial, and anticancer effects. That suggests that lichens may be used as possible natural antioxidant, antimicrobial, and anticancer agents.

Authors+Show Affiliations

Department of Biology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia. marijanakosanic@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22260138

Citation

Kosanić, Marijana, et al. "Antioxidant, Antimicrobial, and Anticancer Activity of 3 Umbilicaria Species." Journal of Food Science, vol. 77, no. 1, 2012, pp. T20-5.
Kosanić M, Ranković B, Stanojković T. Antioxidant, antimicrobial, and anticancer activity of 3 Umbilicaria species. J Food Sci. 2012;77(1):T20-5.
Kosanić, M., Ranković, B., & Stanojković, T. (2012). Antioxidant, antimicrobial, and anticancer activity of 3 Umbilicaria species. Journal of Food Science, 77(1), T20-5. https://doi.org/10.1111/j.1750-3841.2011.02459.x
Kosanić M, Ranković B, Stanojković T. Antioxidant, Antimicrobial, and Anticancer Activity of 3 Umbilicaria Species. J Food Sci. 2012;77(1):T20-5. PubMed PMID: 22260138.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, antimicrobial, and anticancer activity of 3 Umbilicaria species. AU - Kosanić,Marijana, AU - Ranković,Branislav, AU - Stanojković,Tatjana, Y1 - 2011/11/10/ PY - 2012/1/21/entrez PY - 2012/1/21/pubmed PY - 2012/6/13/medline SP - T20 EP - 5 JF - Journal of food science JO - J Food Sci VL - 77 IS - 1 N2 - The aim of this study is to investigate in vitro antioxidant, antimicrobial, and anticancer activity of the acetone extracts of the lichens Umbilicaria crustulosa, U. cylindrica, and U. polyphylla. Antioxidant activity was evaluated by 5 separate methods: free radical scavenging, superoxide anion radical scavenging, reducing power, determination of total phenolic compounds, and determination of total flavonoid content. Of the lichens tested, U. polyphylla had largest free radical scavenging activity (72.79% inhibition at a concentration of 1 mg/mL), which was similar as standard antioxidants in the same concentration. Moreover, the tested extracts had effective reducing power and superoxide anion radical scavenging. Total content of phenol and flavonoid in extracts was determined as pyrocatechol equivalent, and as rutin equivalent, respectively. The strong relationships between total phenolic and flavonoid contents and the antioxidant effect of tested extracts were observed. The antimicrobial activity was estimated by determination of the minimal inhibitory concentration by the broth microdilution method. The most active was extract of U. polyphylla with minimum inhibitory concentration values ranging from 1.56 to 12.5 mg/mL. Anticancer activity was tested against FemX (human melanoma) and LS174 (human colon carcinoma) cell lines using MTT method. All extracts were found to be strong anticancer activity toward both cell lines with IC₅₀ values ranging from 28.45 to 97.82 μg/mL. The present study shows that tested lichen extracts demonstrated a strong antioxidant, antimicrobial, and anticancer effects. That suggests that lichens may be used as possible natural antioxidant, antimicrobial, and anticancer agents. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/22260138/Antioxidant_antimicrobial_and_anticancer_activity_of_3_Umbilicaria_species_ L2 - https://doi.org/10.1111/j.1750-3841.2011.02459.x DB - PRIME DP - Unbound Medicine ER -