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Aggregation effects of gold nanoparticles for single-base mismatch detection in influenza A (H1N1) DNA sequences using fluorescence and Raman measurements.
Colloids Surf B Biointerfaces. 2012 May 01; 93:148-53.CS

Abstract

Aggregation effects of gold nanoparticles (AuNPs) were examined for the discrimination of single point mutations through the hybridization of oligonucleotides (25-50 nM) modified with a fluorescent Texas red dye. The sequences of oligonucleotides were designed to detect the H1N1 virus gene. Single-base mismatch detection due to different adsorption propensities of oligonucleotides could be achieved using fluorescence quenching and surface-enhanced Raman scattering (SERS) properties of the dye. We observed that the addition of perfectly matched double stranded DNA (pmdsDNA), modified with the Texas red dye in the suspension of citrate-reduced AuNPs could increase fluorescence recovery intensities more substantially than either single-base mismatched double stranded DNA (sbmdsDNA) or single stranded DNA (ssDNA). We also tested DNA hybridization under both aggregation and near non-aggregation conditions for fluorescence measurements. A spectral difference in fluorescence intensity between pmdsDNA and sbmdsDNA appeared to be more discriminating under near non-aggregation than aggregation conditions. On the other hand, the SERS intensities of pmdsDNA and sbmdsDNA decreased more significantly than that of ssDNA under aggregation conditions, whereas we could not observe any SERS intensities under non-aggregation conditions.

Authors+Show Affiliations

Department of Chemistry, Soongsil University, Seoul 156-743, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22261178

Citation

Ganbold, Erdene-Ochir, et al. "Aggregation Effects of Gold Nanoparticles for Single-base Mismatch Detection in Influenza a (H1N1) DNA Sequences Using Fluorescence and Raman Measurements." Colloids and Surfaces. B, Biointerfaces, vol. 93, 2012, pp. 148-53.
Ganbold EO, Kang T, Lee K, et al. Aggregation effects of gold nanoparticles for single-base mismatch detection in influenza A (H1N1) DNA sequences using fluorescence and Raman measurements. Colloids Surf B Biointerfaces. 2012;93:148-53.
Ganbold, E. O., Kang, T., Lee, K., Lee, S. Y., & Joo, S. W. (2012). Aggregation effects of gold nanoparticles for single-base mismatch detection in influenza A (H1N1) DNA sequences using fluorescence and Raman measurements. Colloids and Surfaces. B, Biointerfaces, 93, 148-53. https://doi.org/10.1016/j.colsurfb.2011.12.026
Ganbold EO, et al. Aggregation Effects of Gold Nanoparticles for Single-base Mismatch Detection in Influenza a (H1N1) DNA Sequences Using Fluorescence and Raman Measurements. Colloids Surf B Biointerfaces. 2012 May 1;93:148-53. PubMed PMID: 22261178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aggregation effects of gold nanoparticles for single-base mismatch detection in influenza A (H1N1) DNA sequences using fluorescence and Raman measurements. AU - Ganbold,Erdene-Ochir, AU - Kang,Taegyeong, AU - Lee,Kangtaek, AU - Lee,So Yeong, AU - Joo,Sang-Woo, Y1 - 2011/12/31/ PY - 2011/04/10/received PY - 2011/12/09/revised PY - 2011/12/23/accepted PY - 2012/1/21/entrez PY - 2012/1/21/pubmed PY - 2012/6/26/medline SP - 148 EP - 53 JF - Colloids and surfaces. B, Biointerfaces JO - Colloids Surf B Biointerfaces VL - 93 N2 - Aggregation effects of gold nanoparticles (AuNPs) were examined for the discrimination of single point mutations through the hybridization of oligonucleotides (25-50 nM) modified with a fluorescent Texas red dye. The sequences of oligonucleotides were designed to detect the H1N1 virus gene. Single-base mismatch detection due to different adsorption propensities of oligonucleotides could be achieved using fluorescence quenching and surface-enhanced Raman scattering (SERS) properties of the dye. We observed that the addition of perfectly matched double stranded DNA (pmdsDNA), modified with the Texas red dye in the suspension of citrate-reduced AuNPs could increase fluorescence recovery intensities more substantially than either single-base mismatched double stranded DNA (sbmdsDNA) or single stranded DNA (ssDNA). We also tested DNA hybridization under both aggregation and near non-aggregation conditions for fluorescence measurements. A spectral difference in fluorescence intensity between pmdsDNA and sbmdsDNA appeared to be more discriminating under near non-aggregation than aggregation conditions. On the other hand, the SERS intensities of pmdsDNA and sbmdsDNA decreased more significantly than that of ssDNA under aggregation conditions, whereas we could not observe any SERS intensities under non-aggregation conditions. SN - 1873-4367 UR - https://www.unboundmedicine.com/medline/citation/22261178/Aggregation_effects_of_gold_nanoparticles_for_single_base_mismatch_detection_in_influenza_A__H1N1__DNA_sequences_using_fluorescence_and_Raman_measurements_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0927-7765(11)00766-1 DB - PRIME DP - Unbound Medicine ER -