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Sub-lethal levels of carvacrol reduce Salmonella Typhimurium motility and invasion of porcine epithelial cells.
Vet Microbiol. 2012 May 25; 157(1-2):200-7.VM

Abstract

The European ban on the use of antibiotic growth promotors has increased the search for new alternatives to prevent pig intestinal microbial diseases and to stimulate growth. The addition of essential oils or components thereof, such as carvacrol, to pig feed is a promising alternative. In this report we determined the effect of sub-lethal concentrations of carvacrol on Salmonella Typhimurium. At concentrations where growth of Salmonella was not inhibited, carvacrol completely inhibited motility of the bacterium. This loss of motility was not due to the loss of the flagellum or to ATP shortage upon carvacrol treatment. Adhesion of Salmonella to IPEC-J2, porcine intestinal epithelial cells, was not affected by carvacrol but invasion was significantly reduced. In addition, the epithelial gene expression of porcine β-defensin 2, an innate immune response to Salmonella infection, was reduced when Salmonella was exposed to carvacrol. This indicates that invasion but not adhesion of Salmonella triggers the porcine β-defensin 2 expression of porcine epithelial cells.

Authors+Show Affiliations

Department of Infectious Diseases and Immunology, Division Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22264386

Citation

Inamuco, Jeanine, et al. "Sub-lethal Levels of Carvacrol Reduce Salmonella Typhimurium Motility and Invasion of Porcine Epithelial Cells." Veterinary Microbiology, vol. 157, no. 1-2, 2012, pp. 200-7.
Inamuco J, Veenendaal AK, Burt SA, et al. Sub-lethal levels of carvacrol reduce Salmonella Typhimurium motility and invasion of porcine epithelial cells. Vet Microbiol. 2012;157(1-2):200-7.
Inamuco, J., Veenendaal, A. K., Burt, S. A., Post, J. A., Tjeerdsma-van Bokhoven, J. L., Haagsman, H. P., & Veldhuizen, E. J. (2012). Sub-lethal levels of carvacrol reduce Salmonella Typhimurium motility and invasion of porcine epithelial cells. Veterinary Microbiology, 157(1-2), 200-7. https://doi.org/10.1016/j.vetmic.2011.12.021
Inamuco J, et al. Sub-lethal Levels of Carvacrol Reduce Salmonella Typhimurium Motility and Invasion of Porcine Epithelial Cells. Vet Microbiol. 2012 May 25;157(1-2):200-7. PubMed PMID: 22264386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sub-lethal levels of carvacrol reduce Salmonella Typhimurium motility and invasion of porcine epithelial cells. AU - Inamuco,Jeanine, AU - Veenendaal,Andreas K J, AU - Burt,Sara A, AU - Post,Jan A, AU - Tjeerdsma-van Bokhoven,Johanna L M, AU - Haagsman,Henk P, AU - Veldhuizen,Edwin J A, Y1 - 2011/12/24/ PY - 2011/01/26/received PY - 2011/12/14/revised PY - 2011/12/19/accepted PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/11/6/medline SP - 200 EP - 7 JF - Veterinary microbiology JO - Vet Microbiol VL - 157 IS - 1-2 N2 - The European ban on the use of antibiotic growth promotors has increased the search for new alternatives to prevent pig intestinal microbial diseases and to stimulate growth. The addition of essential oils or components thereof, such as carvacrol, to pig feed is a promising alternative. In this report we determined the effect of sub-lethal concentrations of carvacrol on Salmonella Typhimurium. At concentrations where growth of Salmonella was not inhibited, carvacrol completely inhibited motility of the bacterium. This loss of motility was not due to the loss of the flagellum or to ATP shortage upon carvacrol treatment. Adhesion of Salmonella to IPEC-J2, porcine intestinal epithelial cells, was not affected by carvacrol but invasion was significantly reduced. In addition, the epithelial gene expression of porcine β-defensin 2, an innate immune response to Salmonella infection, was reduced when Salmonella was exposed to carvacrol. This indicates that invasion but not adhesion of Salmonella triggers the porcine β-defensin 2 expression of porcine epithelial cells. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/22264386/Sub_lethal_levels_of_carvacrol_reduce_Salmonella_Typhimurium_motility_and_invasion_of_porcine_epithelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(11)00661-4 DB - PRIME DP - Unbound Medicine ER -