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Green-to-red photoconvertible mEosFP-aided live imaging in plants.
Methods Enzymol. 2012; 504:163-81.ME

Abstract

Numerous subcellular-targeted probes have been created using a monomeric green-to-red photoconvertible Eos fluorescent protein for understanding the growth and development of plants. These probes can be used to create color-based differentiation between similar cells, differentially label organelle subpopulations, and track subcellular structures and their interactions. Both green and red fluorescent forms of mEosFP are stable and compatible with single colored FPs. Differential highlighting using mEosFP probes greatly increases spatiotemporal precision during live imaging.

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Authors+Show Affiliations

Mathur J
Department of Molecular and Cellular Biology, Laboratory of Plant Development and Interactions, University of Guelph, Guelph, Canada.
Griffiths S
No affiliation info available
Barton K
No affiliation info available
Schattat MH
No affiliation info available

MeSH

ArabidopsisCell TrackingFluorescence Recovery After PhotobleachingGreen Fluorescent ProteinsImage Processing, Computer-AssistedLuminescent ProteinsPlant CellsPlants, Genetically Modified

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22264534

Citation

Mathur, Jaideep, et al. "Green-to-red Photoconvertible mEosFP-aided Live Imaging in Plants." Methods in Enzymology, vol. 504, 2012, pp. 163-81.
Mathur J, Griffiths S, Barton K, et al. Green-to-red photoconvertible mEosFP-aided live imaging in plants. Methods Enzymol. 2012;504:163-81.
Mathur, J., Griffiths, S., Barton, K., & Schattat, M. H. (2012). Green-to-red photoconvertible mEosFP-aided live imaging in plants. Methods in Enzymology, 504, 163-81. https://doi.org/10.1016/B978-0-12-391857-4.00008-2
Mathur J, et al. Green-to-red Photoconvertible mEosFP-aided Live Imaging in Plants. Methods Enzymol. 2012;504:163-81. PubMed PMID: 22264534.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green-to-red photoconvertible mEosFP-aided live imaging in plants. AU - Mathur,Jaideep, AU - Griffiths,Sarah, AU - Barton,Kiah, AU - Schattat,Martin H, PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/6/20/medline SP - 163 EP - 81 JF - Methods in enzymology JO - Methods Enzymol VL - 504 N2 - Numerous subcellular-targeted probes have been created using a monomeric green-to-red photoconvertible Eos fluorescent protein for understanding the growth and development of plants. These probes can be used to create color-based differentiation between similar cells, differentially label organelle subpopulations, and track subcellular structures and their interactions. Both green and red fluorescent forms of mEosFP are stable and compatible with single colored FPs. Differential highlighting using mEosFP probes greatly increases spatiotemporal precision during live imaging. SN - 1557-7988 UR - https://www.unboundmedicine.com/medline/citation/22264534/Green_to_red_photoconvertible_mEosFP_aided_live_imaging_in_plants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/B978-0-12-391857-4.00008-2 DB - PRIME DP - Unbound Medicine ER -