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Green-to-red photoconvertible mEosFP-aided live imaging in plants.
Methods Enzymol. 2012; 504:163-81.ME

Abstract

Numerous subcellular-targeted probes have been created using a monomeric green-to-red photoconvertible Eos fluorescent protein for understanding the growth and development of plants. These probes can be used to create color-based differentiation between similar cells, differentially label organelle subpopulations, and track subcellular structures and their interactions. Both green and red fluorescent forms of mEosFP are stable and compatible with single colored FPs. Differential highlighting using mEosFP probes greatly increases spatiotemporal precision during live imaging.

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  • Publisher Full Text

    • Authors+Show Affiliations

    Mathur J
    Department of Molecular and Cellular Biology, Laboratory of Plant Development and Interactions, University of Guelph, Guelph, Canada.
    Griffiths S
    No affiliation info available
    Barton K
    No affiliation info available
    Schattat MH
    No affiliation info available

    MeSH

    ArabidopsisCell TrackingFluorescence Recovery After PhotobleachingGreen Fluorescent ProteinsImage Processing, Computer-AssistedLuminescent ProteinsPlant CellsPlants, Genetically Modified

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22264534

    Citation

    Mathur, Jaideep, et al. "Green-to-red Photoconvertible mEosFP-aided Live Imaging in Plants." Methods in Enzymology, vol. 504, 2012, pp. 163-81.
    Mathur J, Griffiths S, Barton K, et al. Green-to-red photoconvertible mEosFP-aided live imaging in plants. Meth Enzymol. 2012;504:163-81.
    Mathur, J., Griffiths, S., Barton, K., & Schattat, M. H. (2012). Green-to-red photoconvertible mEosFP-aided live imaging in plants. Methods in Enzymology, 504, 163-81. https://doi.org/10.1016/B978-0-12-391857-4.00008-2
    Mathur J, et al. Green-to-red Photoconvertible mEosFP-aided Live Imaging in Plants. Meth Enzymol. 2012;504:163-81. PubMed PMID: 22264534.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Green-to-red photoconvertible mEosFP-aided live imaging in plants. AU - Mathur,Jaideep, AU - Griffiths,Sarah, AU - Barton,Kiah, AU - Schattat,Martin H, PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/6/20/medline SP - 163 EP - 81 JF - Methods in enzymology JO - Meth. Enzymol. VL - 504 N2 - Numerous subcellular-targeted probes have been created using a monomeric green-to-red photoconvertible Eos fluorescent protein for understanding the growth and development of plants. These probes can be used to create color-based differentiation between similar cells, differentially label organelle subpopulations, and track subcellular structures and their interactions. Both green and red fluorescent forms of mEosFP are stable and compatible with single colored FPs. Differential highlighting using mEosFP probes greatly increases spatiotemporal precision during live imaging. SN - 1557-7988 UR - https://www.unboundmedicine.com/medline/citation/22264534/Green_to_red_photoconvertible_mEosFP_aided_live_imaging_in_plants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/B978-0-12-391857-4.00008-2 DB - PRIME DP - Unbound Medicine ER -