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Live attenuated influenza virus (LAIV) induces different mucosal T cell function in nonsmokers and smokers.

Abstract

Smokers are more susceptible to respiratory infections, including influenza. To explore the effect of smoking on influenza-induced responses within the nasal mucosa, we have developed a protocol using inoculation with live attenuated influenza virus (LAIV) vaccine followed by sampling of the nasal mucosa. Mucosal cell populations were harvested through superficial biopsy of the nasal inferior turbinate pre and post LAIV inoculation and analyzed using flow cytometry. The majority of nasal biopsy CD45+ immune cells at baseline were CD3+ T lymphocytes. Following LAIV, these lymphocytes increased in nonsmokers but not in smokers. A subset of individuals was negative for helper T cell marker CD4 and cytotoxic T cell marker CD8 but positive for the γδ T cell receptor (TCR). Increases in γδ TCR+ cells were greater in nonsmokers, than in smokers. Thus, LAIV-induced changes in CD3 T as well as γδ T lymphocyte percentages are suppressed in smokers compared to nonsmokers.

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  • Authors+Show Affiliations

    ,

    Curriculum in Toxicology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7127, USA.

    , , ,

    Source

    Clinical immunology (Orlando, Fla.) 142:3 2012 Mar pg 232-6

    MeSH

    Adolescent
    Adult
    Biopsy
    Cell Survival
    Female
    Humans
    Immunity, Mucosal
    Influenza Vaccines
    Male
    Prospective Studies
    Smoking
    T-Lymphocytes
    Vaccines, Attenuated
    Young Adult

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22264637

    Citation

    Horvath, Katherine M., et al. "Live Attenuated Influenza Virus (LAIV) Induces Different Mucosal T Cell Function in Nonsmokers and Smokers." Clinical Immunology (Orlando, Fla.), vol. 142, no. 3, 2012, pp. 232-6.
    Horvath KM, Brighton LE, Herbst M, et al. Live attenuated influenza virus (LAIV) induces different mucosal T cell function in nonsmokers and smokers. Clin Immunol. 2012;142(3):232-6.
    Horvath, K. M., Brighton, L. E., Herbst, M., Noah, T. L., & Jaspers, I. (2012). Live attenuated influenza virus (LAIV) induces different mucosal T cell function in nonsmokers and smokers. Clinical Immunology (Orlando, Fla.), 142(3), pp. 232-6. doi:10.1016/j.clim.2011.12.013.
    Horvath KM, et al. Live Attenuated Influenza Virus (LAIV) Induces Different Mucosal T Cell Function in Nonsmokers and Smokers. Clin Immunol. 2012;142(3):232-6. PubMed PMID: 22264637.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Live attenuated influenza virus (LAIV) induces different mucosal T cell function in nonsmokers and smokers. AU - Horvath,Katherine M, AU - Brighton,Luisa E, AU - Herbst,Margaret, AU - Noah,Terry L, AU - Jaspers,Ilona, Y1 - 2012/01/06/ PY - 2011/11/08/received PY - 2011/12/09/revised PY - 2011/12/24/accepted PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/4/13/medline SP - 232 EP - 6 JF - Clinical immunology (Orlando, Fla.) JO - Clin. Immunol. VL - 142 IS - 3 N2 - Smokers are more susceptible to respiratory infections, including influenza. To explore the effect of smoking on influenza-induced responses within the nasal mucosa, we have developed a protocol using inoculation with live attenuated influenza virus (LAIV) vaccine followed by sampling of the nasal mucosa. Mucosal cell populations were harvested through superficial biopsy of the nasal inferior turbinate pre and post LAIV inoculation and analyzed using flow cytometry. The majority of nasal biopsy CD45+ immune cells at baseline were CD3+ T lymphocytes. Following LAIV, these lymphocytes increased in nonsmokers but not in smokers. A subset of individuals was negative for helper T cell marker CD4 and cytotoxic T cell marker CD8 but positive for the γδ T cell receptor (TCR). Increases in γδ TCR+ cells were greater in nonsmokers, than in smokers. Thus, LAIV-induced changes in CD3 T as well as γδ T lymphocyte percentages are suppressed in smokers compared to nonsmokers. SN - 1521-7035 UR - https://www.unboundmedicine.com/medline/citation/22264637/Live_attenuated_influenza_virus__LAIV__induces_different_mucosal_T_cell_function_in_nonsmokers_and_smokers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(11)00368-8 DB - PRIME DP - Unbound Medicine ER -