TY - JOUR T1 - Comparison of extraction procedures for assessment of matrix effect for selective and reliable determination of atazanavir in human plasma by LC-ESI-MS/MS. AU - Yadav,Manish, AU - Trivedi,Vikas, AU - Upadhyay,Vivek, AU - Shah,Gaurang, AU - Baxi,Girin A, AU - Goswami,Sailendra, AU - Shrivastav,Pranav S, Y1 - 2012/01/09/ PY - 2011/09/08/received PY - 2011/12/27/revised PY - 2011/12/28/accepted PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/5/5/medline SP - 138 EP - 49 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 885-886 N2 - A comparative study with three conventional extraction techniques namely protein precipitation (PP), liquid-liquid extraction (LLE) and solid phase extraction (SPE) has been demonstrated to assess the magnitude of matrix interference by post-column analyte infusion and post extraction analyte spiking for the determination of atazanavir from human plasma. Severe ion suppression observed in PP and to a lesser extent in LLE was circumvented by SPE on LiChrosep Sequence extraction cartridge. Based on these observations a selective, rugged and high throughput SPE-LC-MS/MS method has been developed for reliable determination of atazanavir in human plasma. The chromatographic separation was achieved on a Hypersil Gold C18 (50mm×4.6mm, 5μm) analytical column using 5mM ammonium formate in water:methanol (10:90, v/v) as the mobile phase under isocratic conditions. The method was validated over a wide dynamic concentration range of 10-6000ng/mL. The mean relative recovery and absolute matrix effect across quality controls were 84.9 and 93.2%, respectively. The precision value for relative matrix effect between eight different lots of plasma, expressed as %CV of the slopes of the calibration lines was 2.41. The stability of atazanavir under different storage conditions varied from -8.4 to 5.4%. The method was successfully applied to a bioequivalence study of 300mg atazanavir capsule formulation in 24 healthy Indian males under fasting condition. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/22265353/Comparison_of_extraction_procedures_for_assessment_of_matrix_effect_for_selective_and_reliable_determination_of_atazanavir_in_human_plasma_by_LC_ESI_MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(12)00018-9 DB - PRIME DP - Unbound Medicine ER -