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Determination of rutin and quercetin in Chinese herbal medicine by ionic liquid-based pressurized liquid extraction-liquid chromatography-chemiluminescence detection.
Talanta. 2012 Jan 15; 88:222-9.T

Abstract

A novel ionic liquid-based pressurized liquid extraction (IL-PLE) procedure coupled with high performance liquid chromatography (HPLC) tandem chemiluminescence (CL) detection capable of quantifying trace amounts of rutin and quercetin in four Chinese medicine plants including Flos sophorae Immaturus, Crateagus pinnatifida Bunge, Hypericum japonicum Thunb and Folium Mori was described in this paper. To avoid environmental pollution and toxicity to the operators, ionic liquids (ILs), 1-alkyl-3-methylimidazolium chloride ([C(n)mim][Cl]) aqueous solutions were used in the PLE procedure as extractants replacing traditional organic solvents. In addition, chemiluminescence detection was utilized for its minimal interference from endogenous components of complex matrix. Parameters affecting extraction and analysis were carefully optimized. Compared with the conventional ultrasonic-assisted extraction (UAE) and heat-reflux extraction (HRE), the optimized method achieved the highest extraction efficiency in the shortest extraction time with the least solvent consumption. The applicability of the proposed method to real sample was confirmed. Under the optimized conditions, good reproducibility of extraction performance was obtained and good linearity was observed with correlation coefficients (r) between 0.9997 and 0.9999. The detection limits of rutin and quercetin (LOD, S/N=3) were 1.1×10(-2)mg/L and 3.8×10(-3)mg/L, respectively. The average recoveries of rutin and quercetin for real samples were 93.7-105% with relative standard deviation (RSD) lower than 5.7%. To the best of our knowledge, this paper is the first contribution to utilize a combination of IL-PLE with chemiluminescence detection. And the experimental results indicated that the proposed method shows a promising prospect in extraction and determination of rutin and quercetin in medicinal plants.

Authors+Show Affiliations

Department of Chemistry, Xixi Campus, Zhejiang University, Hangzhou 310028, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22265491

Citation

Wu, Hongwei, et al. "Determination of Rutin and Quercetin in Chinese Herbal Medicine By Ionic Liquid-based Pressurized Liquid Extraction-liquid Chromatography-chemiluminescence Detection." Talanta, vol. 88, 2012, pp. 222-9.
Wu H, Chen M, Fan Y, et al. Determination of rutin and quercetin in Chinese herbal medicine by ionic liquid-based pressurized liquid extraction-liquid chromatography-chemiluminescence detection. Talanta. 2012;88:222-9.
Wu, H., Chen, M., Fan, Y., Elsebaei, F., & Zhu, Y. (2012). Determination of rutin and quercetin in Chinese herbal medicine by ionic liquid-based pressurized liquid extraction-liquid chromatography-chemiluminescence detection. Talanta, 88, 222-9. https://doi.org/10.1016/j.talanta.2011.10.036
Wu H, et al. Determination of Rutin and Quercetin in Chinese Herbal Medicine By Ionic Liquid-based Pressurized Liquid Extraction-liquid Chromatography-chemiluminescence Detection. Talanta. 2012 Jan 15;88:222-9. PubMed PMID: 22265491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of rutin and quercetin in Chinese herbal medicine by ionic liquid-based pressurized liquid extraction-liquid chromatography-chemiluminescence detection. AU - Wu,Hongwei, AU - Chen,Meilan, AU - Fan,Yunchang, AU - Elsebaei,Fawzi, AU - Zhu,Yan, Y1 - 2011/10/31/ PY - 2011/06/09/received PY - 2011/10/05/revised PY - 2011/10/16/accepted PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/6/5/medline SP - 222 EP - 9 JF - Talanta JO - Talanta VL - 88 N2 - A novel ionic liquid-based pressurized liquid extraction (IL-PLE) procedure coupled with high performance liquid chromatography (HPLC) tandem chemiluminescence (CL) detection capable of quantifying trace amounts of rutin and quercetin in four Chinese medicine plants including Flos sophorae Immaturus, Crateagus pinnatifida Bunge, Hypericum japonicum Thunb and Folium Mori was described in this paper. To avoid environmental pollution and toxicity to the operators, ionic liquids (ILs), 1-alkyl-3-methylimidazolium chloride ([C(n)mim][Cl]) aqueous solutions were used in the PLE procedure as extractants replacing traditional organic solvents. In addition, chemiluminescence detection was utilized for its minimal interference from endogenous components of complex matrix. Parameters affecting extraction and analysis were carefully optimized. Compared with the conventional ultrasonic-assisted extraction (UAE) and heat-reflux extraction (HRE), the optimized method achieved the highest extraction efficiency in the shortest extraction time with the least solvent consumption. The applicability of the proposed method to real sample was confirmed. Under the optimized conditions, good reproducibility of extraction performance was obtained and good linearity was observed with correlation coefficients (r) between 0.9997 and 0.9999. The detection limits of rutin and quercetin (LOD, S/N=3) were 1.1×10(-2)mg/L and 3.8×10(-3)mg/L, respectively. The average recoveries of rutin and quercetin for real samples were 93.7-105% with relative standard deviation (RSD) lower than 5.7%. To the best of our knowledge, this paper is the first contribution to utilize a combination of IL-PLE with chemiluminescence detection. And the experimental results indicated that the proposed method shows a promising prospect in extraction and determination of rutin and quercetin in medicinal plants. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/22265491/Determination_of_rutin_and_quercetin_in_Chinese_herbal_medicine_by_ionic_liquid_based_pressurized_liquid_extraction_liquid_chromatography_chemiluminescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(11)00938-6 DB - PRIME DP - Unbound Medicine ER -