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Determination of nicotine exposure in dogs subjected to passive smoking using methanol extraction of hair followed by hydrophilic interaction chromatography in combination with Fourier transform mass spectrometry.
Talanta. 2012 Jan 15; 88:408-11.T

Abstract

There has been no previous assessment of the level of nicotine exposure in companion animals as a result of passive smoking. A method was developed for the determination of nicotine in dog hair where extraction was carried out by sonication in methanol. The levels of nicotine obtained by extraction with methanol were found to be comparable to the lengthier method involving digestion of the hair in 1M NaOH. The methanol extracts were injected directly onto a ZICHILIC column coupled to an Exactive high resolution Fourier Transform mass spectrometer. Endogenous nicotine was quantified against (2)H(4)-nicotine spiked into the extraction medium, linearity was found over a wide range with the calibration curve having a slope close to 1 indicating an equal response for nicotine and the deuterated internal standard, precision was determined to be ±1.9%. Nicotine was present in widely varying amounts in the hair of dogs belonging to smokers and was found to be absent from the hair of dogs belonging to non-smokers. In addition to nicotine, nicotine N-oxides, cotinine, nornicotine and nornicotine N-oxide could be detected in the hair of dogs belonging to smokers. The nicotine N-oxides were only observed in methanol extracts suggesting these compounds are not stable to the NaOH digestion process.

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Authors+Show Affiliations

Bawazeer S
Strathclyde Institute of Pharmacy and Biomedical Sciences, 161 Cathedral Street, Glasgow G4 0RE, United Kingdom.
Watson DG
No affiliation info available
Knottenbelt C
No affiliation info available

MeSH

AnimalsCalibrationChemical FractionationChromatography, LiquidCotinineDeuteriumDogsHairHydrophobic and Hydrophilic InteractionsMethanolNicotineOxidation-ReductionSodium HydroxideSolventsSonicationSpectroscopy, Fourier Transform InfraredTobacco Smoke Pollution

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22265518

Citation

Bawazeer, Saud, et al. "Determination of Nicotine Exposure in Dogs Subjected to Passive Smoking Using Methanol Extraction of Hair Followed By Hydrophilic Interaction Chromatography in Combination With Fourier Transform Mass Spectrometry." Talanta, vol. 88, 2012, pp. 408-11.
Bawazeer S, Watson DG, Knottenbelt C. Determination of nicotine exposure in dogs subjected to passive smoking using methanol extraction of hair followed by hydrophilic interaction chromatography in combination with Fourier transform mass spectrometry. Talanta. 2012;88:408-11.
Bawazeer, S., Watson, D. G., & Knottenbelt, C. (2012). Determination of nicotine exposure in dogs subjected to passive smoking using methanol extraction of hair followed by hydrophilic interaction chromatography in combination with Fourier transform mass spectrometry. Talanta, 88, 408-11. https://doi.org/10.1016/j.talanta.2011.11.008
Bawazeer S, Watson DG, Knottenbelt C. Determination of Nicotine Exposure in Dogs Subjected to Passive Smoking Using Methanol Extraction of Hair Followed By Hydrophilic Interaction Chromatography in Combination With Fourier Transform Mass Spectrometry. Talanta. 2012 Jan 15;88:408-11. PubMed PMID: 22265518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of nicotine exposure in dogs subjected to passive smoking using methanol extraction of hair followed by hydrophilic interaction chromatography in combination with Fourier transform mass spectrometry. AU - Bawazeer,Saud, AU - Watson,David G, AU - Knottenbelt,Clare, Y1 - 2011/11/11/ PY - 2011/08/05/received PY - 2011/10/15/revised PY - 2011/11/01/accepted PY - 2012/1/24/entrez PY - 2012/1/24/pubmed PY - 2012/6/5/medline SP - 408 EP - 11 JF - Talanta JO - Talanta VL - 88 N2 - There has been no previous assessment of the level of nicotine exposure in companion animals as a result of passive smoking. A method was developed for the determination of nicotine in dog hair where extraction was carried out by sonication in methanol. The levels of nicotine obtained by extraction with methanol were found to be comparable to the lengthier method involving digestion of the hair in 1M NaOH. The methanol extracts were injected directly onto a ZICHILIC column coupled to an Exactive high resolution Fourier Transform mass spectrometer. Endogenous nicotine was quantified against (2)H(4)-nicotine spiked into the extraction medium, linearity was found over a wide range with the calibration curve having a slope close to 1 indicating an equal response for nicotine and the deuterated internal standard, precision was determined to be ±1.9%. Nicotine was present in widely varying amounts in the hair of dogs belonging to smokers and was found to be absent from the hair of dogs belonging to non-smokers. In addition to nicotine, nicotine N-oxides, cotinine, nornicotine and nornicotine N-oxide could be detected in the hair of dogs belonging to smokers. The nicotine N-oxides were only observed in methanol extracts suggesting these compounds are not stable to the NaOH digestion process. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/22265518/Determination_of_nicotine_exposure_in_dogs_subjected_to_passive_smoking_using_methanol_extraction_of_hair_followed_by_hydrophilic_interaction_chromatography_in_combination_with_Fourier_transform_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(11)00966-0 DB - PRIME DP - Unbound Medicine ER -